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Analysis of garbage collection network of Colombo district using centrality measures 用中心性方法分析科伦坡地区垃圾收集网络
Q3 Multidisciplinary Pub Date : 2023-10-12 DOI: 10.4038/jsc.v14i1.62
K. A. T. Dewanthi, K. K. K. R. Perera
Waste management is a common problem faced by all developing countries. Colombo city faces the biggest garbage problem than other cities in Sri Lanka. Even through many studies were carried out for waste management problem using different approaches, there were very few research findings were available using graph theoretical approach. In this research, applications of graph theory in garbage collection procedure are depicted. The study mainly focuses on analyzing the garbage collection procedure of Colombo municipal council area in western province through constructing garbage collection network and using centrality measures. Centrality measures are used to compute the importance of any node in a network. Colombo municipal area divides into 6 main administrative districts, and each of these is divided into municipal wards with several junctions and streets. Garbage collection network was initially constructed by assigning a node by a location in google map, and an edge by a street or a path between two locations. Constructed network is an undirected unweighted graph and betweenness, closeness, degree, and eigenvector centrality measures are used to find central locations of the network. By identifying central locations, some machines or recycling trucks can be placed in that central places to deposit the waste. Next, a weighted graph was constructed by taking the weights of an edge as a fraction of weight of collected garbage between two locations. Collected garbage weights and betweenness and degree centrality values for weighted graph are used to identify the shortest paths between central nodes in each municipal ward. Garbage collection trucks can be followed this shortest path in order to reduce their fuel cost and collection time.
废物管理是所有发展中国家面临的共同问题。科伦坡市是斯里兰卡垃圾问题最严重的城市。尽管使用不同的方法对废物管理问题进行了许多研究,但使用图论方法的研究结果很少。本文描述了图论在垃圾回收过程中的应用。本研究主要通过构建垃圾收集网络并采用中心性措施对西部省份科伦坡市政局地区的垃圾收集过程进行分析。中心性度量用于计算网络中任何节点的重要性。科伦坡市区分为6个主要行政区域,每个行政区域又分为市级行政区,并设有若干路口和街道。垃圾收集网络最初是由google地图上的一个位置分配节点,两个位置之间的街道或路径分配边缘来构建的。构建的网络是一个无向无权图,使用中间度、接近度、度和特征向量中心性度量来找到网络的中心位置。通过确定中心位置,一些机器或回收卡车可以放置在中心位置来存放废物。然后,将一条边的权重作为两个位置之间收集的垃圾权重的分数,构造一个加权图。利用收集到的垃圾权值和加权图的中间度和度中心性值来确定各市辖区中心节点之间的最短路径。垃圾收集车可以遵循这条最短路径,以减少燃料成本和收集时间。
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引用次数: 0
Development of novel topical cosmeceutical formulations with antimicrobial activity against acne-causing microorganisms from <em>Coriandrum sativum</em> L. 具有抗菌活性的新型外用药妆制剂对芫荽中引起痤疮的微生物的开发l
Q3 Multidisciplinary Pub Date : 2023-10-12 DOI: 10.4038/jsc.v14i1.60
Nirmani Wishwakala Nawarathne, Kanchana Wijesekera, Gaya Bandara Wijayaratne, Mayuri Napagoda
Spices possess a diverse array of natural phytochemicals with antibacterial, anti-inflammatory, and antioxidant effects. Hence spices could be employed to treat chronic dermatologic conditions like acne vulgaris which involves infection of Propionibacterium acnes and Staphylococcus aureus, and inflammation. Particularly with the emergence of antibiotic resistance, there is an utmost necessity for the development of novel therapeutic agents for the management of acne. Thus, this study was focused on the development of novel topical gel formulations from the seeds of Coriandrum sativum L. (coriander) and to evaluate the antibacterial potential against some acne-causing bacterial species. Initially, the antibacterial effects of the n-hexane, ethyl acetate, and methanol extracts were screened against S. aureus and P. acnes by agar well diffusion assay. Thereafter, ethyl acetate extract of C. sativum was incorporated at predetermined three different concentrations into a novel topical gel base. Agar well diffusion assay and the broth microdilution method were used to evaluate the antibacterial activity of the resulting formulations. Interestingly, all three formulations inhibited the growth of P. acnes and S. aureus, with the highest activity in the formulation comprised of 15% w/w of the seed extract. Furthermore, the antibacterial activity and physical parameters like pH, color, and consistency of these formulations were retained during the storage period of 30 days, demonstrating their suitability as effective therapeutic alternatives in the management of acne vulgaris.
香料含有多种具有抗菌、抗炎和抗氧化作用的天然植物化学物质。因此,香料可以用来治疗慢性皮肤病,如寻常痤疮,包括痤疮丙酸杆菌和金黄色葡萄球菌的感染,以及炎症。特别是随着抗生素耐药性的出现,开发新的治疗痤疮的药物是非常必要的。因此,本研究的重点是从芫荽种子中开发新的外用凝胶制剂,并评估其对一些引起痤疮的细菌的抗菌潜力。首先,通过琼脂孔扩散法筛选正己烷、乙酸乙酯和甲醇提取物对金黄色葡萄球菌和痤疮链球菌的抑菌效果。然后,将苜蓿的乙酸乙酯提取物以预定的三种不同浓度掺入一种新型外用凝胶基中。采用琼脂孔扩散法和肉汤微量稀释法对所得配方进行抑菌活性评价。有趣的是,所有三种配方都能抑制痤疮假单胞菌和金黄色葡萄球菌的生长,其中种子提取物含量为15% w/w的配方活性最高。此外,在30天的储存期内,这些配方的抗菌活性和pH、颜色、一致性等物理参数保持不变,表明它们是治疗寻常痤疮的有效选择。
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引用次数: 0
Design, construction, and performance evaluation of a solar tunnel dryer with an auxiliary flat-plate solar air heater for bitter gourd drying (<em>Momordica charantia</em>) 带辅助平板太阳能空气加热器的苦瓜干燥太阳能隧道式干燥机(&lt;em&gt;苦瓜&lt;/em&gt;)
Q3 Multidisciplinary Pub Date : 2023-10-12 DOI: 10.4038/jsc.v14i1.61
P. Peratheepan, C. Nishan, F. C. Ragel
A solar tunnel dryer and an auxiliary solar air heating unit compatible with the tunnel dryer were designed and constructed. The solar tunnel dryer has a collector chamber and a drying chamber, which are separated by a board with holes in top for air circulation. The drying experiments were conducted for sliced bitter gourd (Momordica charantia) samples in the solar drying system with and without using the auxiliary air heating unit for eight hours in the premises of Eastern University. In order to compare and analyze the performance of the solar drying system, controlled drying experiments were also carried out in open sun on equal mass samples of bitter gourd kept adjacent to the solar drying system. The temperature profiles of the inlet, interface, and outlet of the solar tunnel dryer were compared with ambient temperature, and the wet basis moisture content obtained during the system drying were compared with the open sun drying. The performance of the dryer system has been investigated in terms of temperature profile in the drying area; moisture contents, moisture ratio and effective moisture diffusivity of bitter gourd samples with time of the day. Analysis on bitter gourd samples reveal that the system with the auxiliary air heating unit exhibits enhanced performance compared to the dryer without the auxiliary unit. The moisture ratio decreases exponentially as predicted by logarithmic model. The effective moisture diffusivity values of bitter gourd drying were determined based on Fick’s Second Diffusion model.
设计并建造了太阳能隧道式干燥机及其配套的太阳能空气加热装置。太阳能隧道式干燥机具有集热室和干燥室,其由顶部有孔的板隔开,用于空气循环。在东方大学室内,对苦瓜切片样品在有和不使用辅助空气加热装置的太阳能干燥系统中进行了8小时的干燥实验。为了对太阳能干燥系统的性能进行比较和分析,还对放置在太阳能干燥系统旁边的等质量苦瓜样品进行了露天对照干燥试验。对太阳能隧道式干燥机的入口、界面和出口温度分布与环境温度进行了比较,并对系统干燥过程中获得的湿基水分含量与开放式太阳干燥进行了比较。干燥系统的性能在干燥区域的温度分布方面进行了研究;苦瓜样品含水率、含水率和有效水分扩散率随时间的变化。对苦瓜样品的分析表明,与没有辅助装置的干燥机相比,有辅助空气加热装置的系统表现出更高的性能。根据对数模型预测,含水率呈指数递减。利用菲克第二扩散模型确定了苦瓜干燥过程中水分的有效扩散系数。
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引用次数: 0
Bacterial and Fungal Community Profiling of Karst Ecosystem in Basey, Samar, Philippines Using Shotgun Metagenomic Approach 利用射枪元基因组学方法分析菲律宾萨马尔省 Basey 喀斯特生态系统的细菌和真菌群落特征
Q3 Multidisciplinary Pub Date : 2023-07-26 DOI: 10.56899/152.05.17
John Daniel Ong, Edmerson Geronimo, E. F. Bosito, Kristiann Gabriele Villarama, Zachary Lara, Florabelle Querubin, M. Balolong
The Philippines has an abundance of karst or forest over limestone landscapes, but only a small percentage of them have been studied and protected. Although the flora and fauna of karst forests are diverse and unique, little is known about the microflora that inhabits this ecosystem. The taxonomic and functional composition of bacteria and fungi in soil samples from three locations within three karst forest zones in Basey, Samar, Philippines were analyzed using Illumina shotgun metagenome sequencing. Proteobacteria and Actinobacteria bacterial sequences were most prevalent in the karst soil, followed by those of the Firmicutes, Acidobacteria, Chloroflexi, Planctomycetes, Cyanobacteria, Verrumicrobia, Bacteriodetes, and Deinococcus-Thermus. The most abundant fungal sequences belonged to Ascomycota, followed by Basidiomycota. An average of 33 million predicted protein features was detected across all sites. Enzyme pathways for nitrogen and sulfur metabolism, and several carbon fixation pathways, appeared nearly complete. To our knowledge, this is the first report to provide baseline information on the microbial community and their possible roles in karst forest ecosystem health in the Philippines, which may lead to identifying new microbes with specialized metabolism and promoting biodiversity conservation of karst forests in the Philippines. Furthermore, correlation analysis with plant diversity will reveal plant-microbe interaction leading to the understanding of the adaptation, abundance, survival, and diversity of microorganisms and plants.
菲律宾拥有丰富的岩溶或石灰岩森林景观,但其中只有一小部分得到了研究和保护。虽然喀斯特森林的动植物种类繁多且独一无二,但人们对栖息在这种生态系统中的微生物却知之甚少。本研究利用 Illumina 猎枪元基因组测序技术分析了菲律宾萨马尔省 Basey 三个喀斯特森林区三个地点的土壤样本中细菌和真菌的分类和功能组成。岩溶土壤中最常见的细菌序列是蛋白细菌和放线菌,其次是固着菌、酸性细菌、绿霉菌、扁孢霉菌、蓝藻菌、蛭形菌、细菌和脱球菌。最多的真菌序列属于子囊菌目,其次是担子菌目。所有地点平均检测到 3,300 万个预测的蛋白质特征。氮和硫代谢的酶通路以及几种碳固定通路已基本完成。据我们所知,这是第一份提供菲律宾岩溶森林微生物群落基线信息及其在岩溶森林生态系统健康中可能发挥的作用的报告,可能有助于发现具有特殊代谢的新微生物,促进菲律宾岩溶森林的生物多样性保护。此外,与植物多样性的相关分析将揭示植物与微生物之间的相互作用,从而了解微生物和植物的适应性、丰度、生存和多样性。
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引用次数: 0
Simulated Docking of alpha-Conotoxin Interactions with Nicotinic Acetylcholine Receptors alpha-Conotoxin 与烟碱乙酰胆碱受体相互作用的模拟对接
Q3 Multidisciplinary Pub Date : 2023-07-24 DOI: 10.56899/152.05.15
Neil Andrew Bascos, Ron Michael Acda, Clarence Lei Bautista, Elsie Jimenez
The nicotinic acetylcholine receptors (nAChRs) are linked to several biological processes and neurological conditions. The α-conotoxins represent an essential resource for ligands with which to explore structure-function correlations for a variety of nAChR subtypes. With the multitude of nAChR subtypes occurring in vivo, it is imperative to search for subtype-selective ligands to effectively utilize their modulatory functions for their target receptors. In this work,docking simulations using PatchDock showed that either the replacement of Ala10 with the more hydrophobic Leu in PnIA to PnIA[A10L] or the presence of a sulfated tyrosine in the structural scaffold of PnIA[Y15Y] or PnIB[Y15Y] (Y stands for sulfated tyrosine) is sufficient to alter the interactions of α-conotoxins PnIA and PnIB for their target nAChR receptor subtypes – α7and α3β2. The employed protocol can be utilized to screen for ligand docking and amino acid residue interactions of α-conotoxins to certain nAChR subtypes. This was tested on α-conotoxinLoIA, which was predicted to preferentially bind to the α3β2 nAChR over α7 nAChR. The present work is supportive of the idea that the conserved α-conotoxin structural scaffold can be further investigated to guide the synthesis of novel nAChR ligands with greater specificity.The results of this study will be useful in the rational design of selective nAChR antagonists for potential peptide therapeutics.
烟碱乙酰胆碱受体(nAChR)与多种生物过程和神经系统疾病有关。α-芋螺毒素是探索各种 nAChR 亚型结构-功能相关性的重要配体资源。由于体内存在多种 nAChR 亚型,因此必须寻找亚型选择性配体,以有效利用它们对靶受体的调节功能。在这项工作中PnIA[A10L]或 PnIA[Y15Y] 或 PnIB[Y15Y](Y 代表硫酸化酪氨酸)结构支架中硫酸化酪氨酸的存在足以改变α-考托毒素 PnIA 和 PnIB 与其目标 nAChR 受体亚型--α7 和 α3β2--的相互作用。所采用的方案可用于筛选α-共毒素与某些 nAChR 亚型的配体对接和氨基酸残基相互作用。我们对 α-conotoxinLoIA 进行了测试,根据预测,α-conotoxinLoIA 与 α3β2 nAChR 的结合优于与 α7 nAChR 的结合。本研究的结果将有助于为潜在的多肽疗法合理设计选择性 nAChR 拮抗剂。
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引用次数: 0
Community-Academic Partnership in Action: Results of a Pilot Diabetes Intervention Program in Rural Southern Philippines 行动中的社区-学术合作:菲律宾南部农村地区糖尿病干预试点计划的成果
Q3 Multidisciplinary Pub Date : 2023-07-24 DOI: 10.56899/152.05.16
Cristela Mae C. Candelario, JC Louise Bandala-Dingal, Melvin Misoles, Josephine Cuajotor
The increasing prevalence of diabetes in the Philippines is a growing public health concern,especially among prediabetic individuals lacking knowledge and awareness about the disease.This study evaluated the effectiveness of a 6-mo community-based health program in a rural area of southern Philippines. The pilot intervention comprised comprehensive learning modules, lectures, discussions, exercises, personalized nutrition counseling, and house-to-house monitoring visits conducted by healthcare professionals affiliated with a higher education institution (HEI), in collaboration with community leaders and health workers. Twenty-two (22) adults with hyperglycemia were enrolled in the study, and their knowledge, attitude, and practices were evaluated before and after the intervention. The majority of participants were aged over 50 (90.91%), were female (86.36%), were married (95.45%), had completed secondary education (50.00%), and were unemployed (59.09%). The results indicated a significant improvement in participants' knowledge (p < 0.001) and practices (p = 0.019). However, the study revealed that attitudes remained predominantly neutral, suggesting challenges in adapting to the condition (p = 0.266). This study underscores the significance of community-academic partnerships and the engagement of community health workers in fostering sustainable health promotion initiatives. Future research endeavors could explore the integration of diverse health promotion strategies to enhance positive attitudes toward diabetes management. The findings of this study can provide valuable insights for larger-scale multisectoral investigations, as well as inform the development of effective and tailored diabetes prevention and management programs in the Philippines and worldwide.
在菲律宾,糖尿病的发病率越来越高,这是一个日益令人担忧的公共卫生问题,尤其是对糖尿病缺乏了解和认识的糖尿病患者。这项研究评估了在菲律宾南部农村地区开展的为期 6 个月的社区保健计划的效果。试点干预措施包括综合学习模块、讲座、讨论、练习、个性化营养咨询以及由高等教育机构(HEI)的医护人员与社区领导和医护人员合作进行的逐户监测访问。22名患有高血糖症的成年人参加了这项研究,并在干预前后对他们的知识、态度和做法进行了评估。大多数参与者的年龄在 50 岁以上(90.91%),女性(86.36%),已婚(95.45%),受过中等教育(50.00%),失业(59.09%)。研究结果表明,参与者在知识(p < 0.001)和实践(p = 0.019)方面均有明显改善。然而,研究表明,参与者的态度仍然以中性为主,这表明他们在适应这种状况方面面临挑战(p = 0.266)。这项研究强调了社区与学术界合作以及社区卫生工作者参与促进可持续健康促进活动的重要性。未来的研究工作可以探索整合多种健康促进策略,以增强人们对糖尿病管理的积极态度。本研究的结果可为更大规模的多部门调查提供宝贵的见解,并为菲律宾和全球制定有效的、量身定制的糖尿病预防和管理计划提供参考。
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引用次数: 0
Gomphonema Species (Bacillariophyceae) from Marikina River, Rizal (Luzon), Philippines 菲律宾吕宋岛马里基纳河的 Gomphonema 种(裸子植物
Q3 Multidisciplinary Pub Date : 2023-07-24 DOI: 10.56899/152.05.11
M. Martinez-Goss, Taisuke Ohtsuka, Harue Inoue, E. Arguelles, Tohru Ikeya, E. Peralta, Rey Donne Papa, Noboru Okuda
A floristic study of the diatoms (Bacillariophyceae) along the Marikina River in the eastern part of Metro Manila, Philippines, recorded at least 140 species belonging to 39 genera. Of these genera, one of the greatest number of species was observed in the genus Gomphonema (18species). These species were identified and described under the bright field light microscope.Some species were further observed under the scanning electron microscope. A habitat description of the occurrence of the Gomphonema species is given. Twelve (12) out of the 18species are new records for the country: G. brasiliense subsp. pacificum Moser, Lange-Bertalot & Metzeltin, G. chinense Liu & Kociolek, G. contraturris Lange-Bertalot & E. Reichardt, G. cuneolus E. Reichardt, G. exilissimum (Grunow) Lange-Bertalot & E.Reichardt, G. insigniforme E.Reichardt & Lange-Bertalot, G. javanicum Hustedt, G. kobayasii Kociolek & J.C.Kingston, G. pseudosphaerophorum H. Kobayasi, G. sundaense E. Reichardt, G. cf. qingyiensis L-X Zhang, P. Yu & Q-M You, and G. vibrio var. bohemicum (Reichelt & Fricke) R. Ross. Hence, this paper reports a total of 44 species of Gomphonema from the Philippines, including previous studies from 1937 to date.
对菲律宾大马尼拉东部马里基纳河沿岸硅藻(Bacillariophyceae)的植物学研究记录了 39 个属的至少 140 个物种。在这些属中,Gomphonema 属(18 个物种)的物种数量最多。在明视野光学显微镜下对这些物种进行了鉴定和描述,并在扫描电子显微镜下对一些物种进行了进一步观察。报告还对 Gomphonema 物种的栖息地进行了描述。18 个物种中有 12 个是该国的新记录:G. brasiliense subsp. pacificum Moser, Lange-Bertalot & Metzeltin、G. chinense Liu & Kociolek、G. contraturris Lange-Bertalot & E. Reichardt、G. cuneolus E. Reichardt、G. exilissimum (Grunow) Lange-Bertalot & E.Reichardt、G. insigniforme E. Reichardt & Lange-Bertalot & E. Reichardt。Reichardt & Lange-Bertalot,G. javanicum Hustedt,G. kobayasii Kociolek & J.C.Kingston,G. pseudosphaerophorum H. Kobayasi,G. sundaense E. Reichardt,G. cf. qingyiensis L-X Zhang,P. Yu & Q-M You,以及 G. vibrio var. bohemicum (Reichelt & Fricke) R. Ross。因此,本文共报告了菲律宾的 44 种 Gomphonema(包括 1937 年至今的研究)。
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引用次数: 0
Influence of Planting Date and Planting Distance on Population Density of Onion Armyworm 种植日期和种植距离对洋葱卷叶蛾种群密度的影响
Q3 Multidisciplinary Pub Date : 2023-07-22 DOI: 10.56899/152.05.06
Christine Joy Manalili, Jasper Neil Mandac, Catherine Joy Garcia, Paula Blanca Aquino, Milagros Diaz, John Dave Aquino, Marilyn Patricio
Onion is an important crop in the province of Nueva Ecija, Philippines. Currently, its productionis being constrained by onion armyworm (OAW; Spodoptera exigua). The impact of plantingdates and distance on the infestation of OAW was studied across two productions. The aim isto evaluate the influence of different combinations of planting dates (November, December, andJanuary) and row spacing [5 cm x 10 cm, 8 cm x 10 cm, 10 cm x 10 cm, and farmer’s practiceor waray (6–10 cm x 6–10 cm)] on the damage and population density of OAW. The count ofobserved OAW larvae from the earliest month (November) and during the month of the usualproduction period (November–December) was negligible to minimal. However, a significantincrease in the OAW population during late planting (January). In terms of leaf damage causedby the OAW feeding, November, December, and January planting had an increasing percentageas the OAW population also increased. There was an increase in bulb diameter as the row spacingwidens (10 cm x 10 cm), as well as an increase in the number of marketable bulb onions whenit is narrow (5 cm x 10 cm). This study showed that planting dates and distances should be oneof the major considerations in onion production and insect pest management.
洋葱是菲律宾新怡诗夏省的一种重要作物。目前,洋葱的生产受到了洋葱甲虫(OAW;Spodoptera exigua)的制约。研究人员在两个生产区研究了种植日期和距离对洋葱尺蠖侵扰的影响。目的是评估不同的播种日期(11 月、12 月和 1 月)和行距组合(5 厘米 x 10 厘米、8 厘米 x 10 厘米、10 厘米 x 10 厘米,以及农民的做法或 waray(6-10 厘米 x 6-10 厘米))对 OAW 的危害和种群密度的影响。从最早的月份(11 月)到通常生产期的月份(11 月至 12 月),观察到的 OAW 幼虫数量可以忽略不计,甚至极少。然而,在种植后期(1 月),OAW 的数量明显增加。就 OAW 摄食造成的叶片损害而言,11 月、12 月和 1 月种植期间,随着 OAW 数量的增加,叶片损害的百分比也在增加。行距越宽(10 厘米 x 10 厘米),鳞茎直径越大,行距越窄(5 厘米 x 10 厘米),可上市鳞茎洋葱的数量也越多。这项研究表明,在洋葱生产和虫害管理中,种植日期和距离应是主要考虑因素之一。
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引用次数: 0
Morphological and Molecular Diversity of Traditional Upland Rice (Oryza sativa L.) Varieties for Drought Tolerance at Seedling Stage 传统陆稻(Oryza sativa L. )苗期抗旱品种的形态和分子多样性
Q3 Multidisciplinary Pub Date : 2023-07-22 DOI: 10.56899/152.05.09
Kim-Lee Domingo, R. Suralta, Verna Dalusong, Nenita Baldo, Reggie dela Cruz, Jorgen Abellera, Joy Jamago
Drought is considered the major threat and most damaging abiotic stress for rice production.However, some traditional upland rice varieties (TURVs) may have some forms of tolerance to various stresses compared to new elite varieties. Two experiments were conducted. Experiment to determine the morpho-physiological response, phenotypic diversity, and similarity of 13 TURVs and 2 check varieties to mild drought stress at the seedling stage (Experiment 1 (E1) and to determine the molecular diversity and genetic similarity of the genotypes using selected simple sequence repeat (SSR) markers ((Experiment 2 (E2). In E1, all TURVs were highly tolerant under mild drought stress for a physiological response. Standardized Shannon-Weaver Diversity Index(H’) showed zero to low phenotypic diversity for qualitative traits. However, the phenotypic diversity of quantitative traits ranged from moderate to high for well-watered conditions, low to high for drought conditions, and moderate to high for re-watered conditions. Cluster analysis through Ward’s minimum variance based on pooled phenotypic data grouped the genotypes into three major clusters at 7 Euclidean distances. For E2, polymorphic information content values from 24 polymorphic SSR markers ranged from slightly informative (RM256) to highly informative (RM206), with a mean of 0.61. Nei’s gene diversity mean was 0.62 and values varied from 0.26 (RM256) to 0.86 (RM206). Based on Jaccard’s similarity coefficient, rice genotypes were grouped into nine minor clusters. The results indicate that TURVs harbor moderate to high allelic diversity and there are potential tolerance genes for mild drought stress that can be utilized in breeding programs for drought-tolerant rice varieties.
干旱被认为是水稻生产的主要威胁和最具破坏性的非生物胁迫。然而,与新的优良品种相比,一些传统的高原水稻品种(TURVs)可能对各种胁迫具有某种形式的耐受性。我们进行了两项实验。实验 1(E1)旨在确定 13 个 TURVs 和 2 个对照品种在幼苗期对轻度干旱胁迫的形态生理反应、表型多样性和相似性;实验 2(E2)旨在利用选定的简单序列重复(SSR)标记确定基因型的分子多样性和遗传相似性。在 E1 试验中,所有 TURV 在轻度干旱胁迫下都具有很强的耐受性,以做出生理反应。标准化香农-韦弗多样性指数(H')显示,质量性状的表型多样性为零或较低。然而,在水分充足的条件下,数量性状的表型多样性从中等到高;在干旱条件下,数量性状的表型多样性从低到高;在水分充足的条件下,数量性状的表型多样性从中等到高。根据汇集的表型数据,通过沃德最小方差进行聚类分析,以 7 个欧氏距离将基因型分为三大类。对于 E2,24 个多态性 SSR 标记的多态性信息含量值从信息量小(RM256)到信息量大(RM206)不等,平均值为 0.61。内氏基因多样性平均值为 0.62,数值从 0.26(RM256)到 0.86(RM206)不等。根据 Jaccard 相似系数,水稻基因型被分为 9 个小聚类。结果表明,TURVs具有中等至较高的等位基因多样性,存在潜在的对轻度干旱胁迫的耐受基因,可用于耐旱水稻品种的育种计划。
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引用次数: 0
Tiny and Everywhere: Microplastic Density and Distribution in Sediments along the West Coast of Lombok Island, Indonesia 微小而无处不在:印度尼西亚龙目岛西海岸沉积物中的微塑料密度与分布
Q3 Multidisciplinary Pub Date : 2023-07-22 DOI: 10.56899/152.05.12
V. Fitrianti, Muhammad Irsyad Abiyusfi Ghafari
Microplastics have recently received high global attention, as their widespread presence can potentially cause physical and toxicological risks to organisms. The west coast of Lombok Island in Indonesia has a high potential for microplastic accumulation; even so, its presence in this area was not well studied. Therefore, this study aimed to describe the distribution and density of microplastics within the sediments on the west coast of Lombok. Microplastic sampling had been carried out in the northern, middle, and southern portions of the west coast of Lombok based on distinct geographical and vegetational characteristics. Sediment samples were taken within the HTL (High Tide Line) zone based on the excavation depths categories, i.e. A (0–2cm), B (2–4 cm), C (4–6 cm), D (6–8 cm), and D (8–10 cm). The sediment samples were filtered using an aluminum sieve with a mesh size of 4.75 mm, targeting microplastic pieces below that size. A total of 16,685 microplastic debris were found distributed horizontally and vertically throughout the station. There was a significant difference in the microplastic density distributed between stations [F(10,22) = 6.96, p < 0.001] and between sediment depths [F(10,44) = 9.07,p < 0.001]. The order of average microplastic concentration was southern > northern > middle stations group. The highest microplastic number recorded in SMP11 (southern station, open bay, high anthropogenic activity, with river and primary mangrove vegetation) was as many as 2,361 (density, D = 125,920.00 ± 204.33 items/m3), whereas SMP01 (northern station, open bay, low anthropogenic activity, and with river and secondary mangrove vegetation) had the lowest microplastic number of 678 (D = 36,160.00 ± 72.82 items/m3). In general, the majority of microplastics (45.76%) were distributed on the sediment surface (A–B), where there was a repeating pattern in the difference of microplastic density fluctuations of about 3% at every 4cm of sediment depth (B–C and D–E), which is thought to be driven by daily tidal fluctuation.There was no clear pattern that indicated whether the specific type of shoreline or the presence of rivers and mangroves had an influence on the level of microplastic accumulation. However,the southern stations group had a considerably higher average of microplastic accumulation.We suspect that the geographical position towards the main ocean current contributes greatly to the microplastic accumulation at the site.
微塑料最近受到了全球的高度关注,因为它们的广泛存在可能会对生物体造成物理和毒理学风险。印度尼西亚龙目岛西海岸极有可能积聚微塑料;尽管如此,对该地区微塑料存在情况的研究并不多。因此,本研究旨在描述龙目岛西海岸沉积物中微塑料的分布和密度。根据不同的地理和植被特征,在龙目岛西海岸的北部、中部和南部进行了微塑料取样。沉积物样本是在高潮线(HTL)区域内根据挖掘深度分类采集的,即 A(0-2 厘米)、B(2-4 厘米)、C(4-6 厘米)、D(6-8 厘米)和 D(8-10 厘米)。沉积物样本使用网眼尺寸为 4.75 毫米的铝筛进行过滤,以筛选网眼尺寸以下的微塑料碎片。共发现 16,685 块微塑料碎片,分布在整个监测站的水平和垂直方向。不同站位之间的微塑料密度分布存在明显差异[F(10,22) = 6.96,p < 0.001],不同沉积深度之间的微塑料密度分布也存在明显差异[F(10,44) = 9.07,p < 0.001]。微塑料平均浓度的顺序为南部 > 北部 > 中间站组。南部站位 11(南部站位,开阔海湾,人为活动频繁,有河流和原生红树林植被)记录到的微塑料数量最多,达 2,361 个(密度,D = 125,920.00 ± 204.33 个/立方米),而北部站位 01(北部站位,开阔海湾,人为活动频繁,有河流和次生红树林植被)记录到的微塑料数量最少,为 678 个(密度,D = 36,160.00 ± 72.82 个/立方米)。总体而言,大部分微塑料(45.76%)分布在沉积物表面(A-B),其中每 4 厘米沉积物深度(B-C 和 D-E)的微塑料密度波动差异约为 3%,这被认为是受每日潮汐波动的影响。不过,南部站点组的微塑料平均累积量要高得多。我们猜测,面向主要洋流的地理位置对该站点的微塑料累积量有很大影响。
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Philippine Journal of Science
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