研究了冷冻和干燥两种方法对山茱萸中酚类、黄酮类、花青素、抗坏血酸总含量及抗氧化活性的影响

Marzieh Piryaei, M. B. Asl, M. M. Abolghasemi
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摘要

对冷冻和干燥的山茱萸极性亚段和非极性亚段的总黄酮、花青素、抗氧化能力、总酚和抗坏血酸含量进行了评价。采用DPPH法、β-胡萝卜素/亚油酸法和还原力法测定样品的抗氧化活性。在DPPH体系中,冷冻水果中甲醇提取物的极性亚组分的自由基清除活性最高(IC50 = 25.15±6.5µg mL-1)。对浸渍法和超声波法两种提取方法进行了比较,结果表明浸渍法的提取效果优于超声波法。这些优势体现在提取黄酮类化合物、花青素、抗氧化能力、酚类和抗坏血酸的总含量等方面。
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The effect of freezing and drying Cornus mas L. from phenols, flavonoids, anthocyanins, and total content of ascorbic acid and antioxidant activity in two methods
Flavonoids, anthocyanins, antioxidant capability, total content of phenolic and ascorbic acid of Cornus mas of frozen and dried for polar and non-polar subfraction were evaluated in this work. Antioxidant activities of the samples were determined by three testing systems, namely, DPPH, β-carotene/linoleic acid, and reducing power assay. In the DPPH system, the highest radical scavenging activity was seen in the polar subfraction of the methanol extract in frozen fruits (IC50 = 25.15 ± 6.5 µg mL–1). Comparison of two methods of extraction, maceration and the ultrasonic method, showed that the results of maceration are better than those of the ultrasonic method. These advantages are visible in extracting the flavonoids, anthocyanins, antioxidant capability, and the total content of phenolic and ascorbic acid.
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