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Graphical biplot assessment of pre-sowing procedures on the seeds of Dracocephalum moldavica L. employing diverse nanoparticles 采用不同纳米颗粒对 Dracocephalum moldavica L.种子播种前程序进行图形双图评估
Pub Date : 2024-05-04 DOI: 10.6001/biologija.2024.70.1.1
N. Sabaghnia, M. Janmohammadi, S. Yousefzadeh
Dracocephalum moldavica L., the Moldavian dragonhead, has found its place in folk medicine and has been used to address kidney complaints. Pre-hydration methodologies were administered to dragonhead seeds utilising nano-silicon dioxide at three concentrations: Si1 0 mM as control, Si2 and Si3, 1 and 2 mM, respectively. Concurrently, treatments with nanoparticles of Fe in oxide form encompassed: Fe1 0 mM as control, Fe2 and Fe3, 1 and 2 mM, respectively. A range of parameters, including the germination percentage, weight (fresh and dry) and length of roots and shoots, seed residue dry weight, and root and shoot dry weights were meticulously gauged. The utilisation of the treatment-by-trait biplot facilitated the visualisation of interrelationships of traits and treatments, with the initial two principal components elucidating 80% of the observed variation. The majority of the traits are located in a specific sector of graph with Si2-Fe3 as the optimal pre-hydration treatment. The ideal treatment for eliciting elevated seed germination properties was detected as Si2-Fe3 (1 mM nano-silicon dioxide and 2 mM of Fe oxide nanoparticles) through the ideal entry biplot. Our findings strongly suggest that priming with silicon and Fe in form of nanoparticles holds the potential to expedite both germination and seedling growth in D. moldavica L. In summary, the text emphasises the potential benefits of nanoparticle technology in agriculture, underscores the importance of precise experimental optimisation, highlights the objectivity of the biplot, and suggests promising strategies for seed priming in the cultivation of the Moldavian dragonhead.
摩尔达维亚龙头(Dracocephalum moldavica L.)在民间医药中占有一席之地,被用于治疗肾脏疾病。利用三种浓度的纳米二氧化硅对龙脑种子进行了水前处理:Si1 浓度为 0 mM,作为对照;Si2 和 Si3 浓度分别为 1 mM 和 2 mM。同时,用氧化物形式的纳米铁粒子进行处理:Fe1 0 mM 作为对照,Fe2 和 Fe3 分别为 1 mM 和 2 mM。对发芽率、根和芽的重量(鲜重和干重)和长度、种子残留干重、根和芽干重等一系列参数进行了细致的测量。利用处理-性状双图谱有助于直观地了解性状和处理之间的相互关系,最初的两个主成分阐明了 80% 的观测变异。大多数性状都位于图中的特定区域,Si2-Fe3 是最佳的水前处理。通过理想的入口双平面图,我们发现 Si2-Fe3(1 毫摩尔纳米二氧化硅和 2 毫摩尔纳米氧化铁)是提高种子萌发特性的理想处理方法。总之,本文强调了纳米颗粒技术在农业中的潜在益处,强调了精确优化实验的重要性,突出了双平面图的客观性,并提出了在摩尔达维亚龙头菜栽培中进行种子诱导的可行策略。
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引用次数: 0
Utilisation of biotic and abiotic components in the ecological assessment of the Ossiomo River, Edo State, Nigeria 在尼日利亚埃多州奥西奥莫河生态评估中利用生物和非生物成分
Pub Date : 2024-05-04 DOI: 10.6001/biologija.2024.70.1.2
E. Biose, N. Egun, David Dauda Yusuf
Fish (Gymnarchus niloticus) and sediment samples were used as bioindicators of heavy metal contamination in the ecological assessment of the Ossiomo River. These samples were collected monthly (August 2021 to January 2022) and analysed for heavy metal content using standard methods. Results from this study showed that both the sediment and fish were contaminated with heavy metals. Heavy metal concentration profile of Fe > Zn > Mn > Cu > Pb > Cr > Cd was observed in the sediment. Bioaccumulation factor (BAF) for heavy metals in G. niloticus were > 1, with an order of Cd > Zn > Mn > Cu > Cr > Fe > Pb (gills); Cu > Cd > Zn > Cr > Mn > Fe > Pb (liver), and Mn > Zn > Cu > Cr > Fe > Cd > Pb (muscles). The pollution load index (PLI) values across the three study stations indicate that there is heavy metal pollution of the sediment (PLI > 1). Potential ecological risk index (PERI) assessment showed that stations 1 and 3 exhibited considerable ecological risk (300 < RI ≤ 600), while station 2 indicated a very high ecological risk (RI > 600). There is a need for periodic water quality assessment and monitoring of human and industrialactivities within the Ossiomo River catchment area in order to forestall further deposition of more pollutants into the river and protect public health.
在奥西奥莫河生态评估中,鱼类(黑线鳕)和沉积物样本被用作重金属污染的生物指标。这些样本每月收集一次(2021 年 8 月至 2022 年 1 月),并采用标准方法对重金属含量进行分析。研究结果表明,沉积物和鱼类都受到了重金属污染。沉积物中的重金属浓度分布为:铁 > 锌 > 锰 > 铜 > 铅 > 铬 > 镉。黑线鳕体内重金属的生物累积系数(BAF)大于 1,顺序为镉>锌>锰>铜>铬>铁>铅(鳃);铜>镉>锌>铬>锰>铁>铅(肝脏);锰>锌>铜>铬>铁>镉>铅(肌肉)。三个研究站的污染负荷指数(PLI)值表明,沉积物存在重金属污染(PLI > 1)。潜在生态风险指数(PERI)评估显示,1 号站和 3 号站的生态风险相当高(300 < RI ≤ 600),而 2 号站的生态风险非常高(RI > 600)。有必要对奥西奥莫河集水区内的人类和工业活动进行定期水质评估和监测,以防止更多污染物进一步沉积到河中,保护公众健康。
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引用次数: 0
Identification of antibodies against Toxocara canis among the members of community X 确定 X 社区成员的犬弓形虫抗体
Pub Date : 2024-05-04 DOI: 10.6001/biologija.2024.70.1.3
Giedrė Jarienė, Loreta Dunderienė, Rugilė Antanavičiūtė, A. Aleksandravičienė
The study aimed to determine the prevalence of toxocariasis among the members of community X with and without dogs and its association with lifestyle factors. The sample size was 88 individuals: 44 who owned dogs and 44 who did not. The ages of the subjects ranged from 18 to 72 years. The prevalence of toxocariasis in community X was 12.5%, with 11 of the 88 individuals having IgG antibodies against Toxocara canis. Factors such as outdoor and indoor dog ownership were statistically significantly associated with the prevalence of toxocariasis (p < 0.05). The prevalence of toxocariasis was observed in different age groups. The youngest person to have antibodies against toxocariasis was 20 years old, while the oldest was 68 years old. The highest percentage of infected people was observed in the age group of 62–72 years.
该研究旨在确定 X 社区有狗和无狗成员中的弓形虫感染率及其与生活方式因素的关系。样本量为 88 人:其中 44 人养狗,44 人不养狗。研究对象的年龄从 18 岁到 72 岁不等。X 社区的弓形虫感染率为 12.5%,88 人中有 11 人有犬弓形虫 IgG 抗体。在统计学上,户外和室内养狗等因素与弓形虫感染率有显著相关性(p < 0.05)。不同年龄段的人都感染了弓形虫病。最年轻的弓形虫抗体携带者为 20 岁,最年长的为 68 岁。62-72 岁年龄组的感染率最高。
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引用次数: 0
Provisional atlas of breeding birds of Walvis Bay in the coastal Namib Desert 纳米布沙漠沿岸沃尔维斯湾繁殖鸟类临时地图集
Pub Date : 2023-12-01 DOI: 10.6001/biologija.2023.69.4.1
Grzegorz Kopij
In austral summer 2016/17, a provisional bird atlas project was conducted in the town of Walvis Bay (~260 ha), Namibia. In total, 18 breeding bird species were recorded. Four eudominant species (Cape Sparrow, Rock Dove, Laughing Dove, and Common Waxbill) comprised together 65.2% of all breeding birds. Three dominant species – House Sparrow, Southern Masked Weaver, and Speckled Pigeon – comprised additional 23.0%. The community dominance index (DI) was = 0.40, Shannon’s Diversity Index H’ = 2.21, Simpson’s Diversity Index D = 0.87, and Pielou’s Evenness Index J’ = 0.76. By far the most numerous feeding guild were granivores (88.0% of all birds recorded) followed by insectivores (6.0%) and frugivores (5.3%). Tree and/or shrub nesting birds comprised 59.5%, while birds nesting in/on buildings the remaining 40.5%. There were neither ground-nesting nor holenesting birds. Structure-wise, the avian community in the town resembles avian communities in other parts of the world (strong dominance of granivores, such as sparrows and doves). In austral summer 2018/19, a total of 25 marine species were recorded in the lagoon, of which nine (36%) were Palearctic migrant and 16 (64%) African residents. In terms of the number of individuals, Palearctic migrants constituted 17% and African residents 83%. More than half of all marine birds were Greater Flamingos, while the most numerous Palearctic species was the Curlew Sandpiper.
2016/17年南部夏季,在纳米比亚沃尔维斯湾镇(约260公顷)进行了一个临时鸟类地图集项目。共录得18种繁殖鸟类。四种优势种(角雀、岩鸽、笑鸽和普通蜡喙)占所有繁殖鸟类的65.2%。三个优势物种——家雀、南方蒙面织布者和斑点鸽——占23.0%。群落优势度指数DI = 0.40, Shannon多样性指数H′= 2.21,Simpson多样性指数D = 0.87, Pielou均匀度指数J′= 0.76。到目前为止,最多的取食类是花岗动物(占所有记录鸟类的88.0%),其次是食虫动物(6.0%)和食果动物(5.3%)。树木和/或灌木筑巢的鸟类占59.5%,而在建筑物上筑巢的鸟类占40.5%。既没有地面筑巢的鸟,也没有筑巢的鸟。从结构上看,该镇的鸟类群落与世界其他地区的鸟类群落相似(花岗岩动物,如麻雀和鸽子的强烈优势)。在2018/19年的南部夏季,泻湖共记录了25种海洋物种,其中9种(36%)是古北移徙动物,16种(64%)是非洲居民。就个人数量而言,古北移民占17%,非洲居民占83%。超过一半的海鸟是大火烈鸟,而数量最多的古北极物种是鸻矶鹬。
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引用次数: 0
Most common vector-borne diseases in dogs in Europe: a review 欧洲最常见的狗病媒传染病:综述
Pub Date : 2023-12-01 DOI: 10.6001/biologija.2023.69.4.3
Karolina Jankauskaitė, G. Zamokas, B. Karvelienė
The wide variety of infectious and parasitic disorders known as vector-borne diseases are spread by blood-feeding parasites such as ticks, fleas, lice, and mosquitoes. Anaplasmosis, ehrlichiosis, babesiosis, and borreliosis are important for both animal welfare and their potential to spread to humans. In addition, several social and environmental factors, such as shifts in the planet temperature and ecosystems, an increase in animal and human mobility, and chemoresistance to insecticides and acaricides are constantly changing the epidemiological scenario of vector-borne diseases. When these arthropods feed on an infected animal, they pick up the disease-causing pathogens. When, subsequently, they feed on a healthy animal, they can transmit the pathogen to that animal through their saliva. Early detection and treatment of these diseases is critical to ensure the best possible outcomes for affected dogs. The aim of the article was to discuss the most common vector-borne diseases in dogs in Europe, looking at the prevalence of the diseases and the health risks for dogs. We attempted to summarise the latest literature on various aspects of the disease pathophysiology, epidemiology, advantages and disadvantages of diagnostic techniques, available treatments, and methods for prevention in dogs.
被称为病媒传播疾病的各种各样的传染病和寄生虫疾病是由吸血寄生虫如蜱、跳蚤、虱子和蚊子传播的。无形体病、埃利希体病、巴贝斯虫病和伯氏螺旋体病对动物福利和它们传播给人类的可能性都很重要。此外,一些社会和环境因素,如地球温度和生态系统的变化,动物和人类流动性的增加,以及对杀虫剂和杀螨剂的化学耐药性,正在不断改变病媒传播疾病的流行病学情况。当这些节肢动物以被感染的动物为食时,它们会携带致病的病原体。随后,当它们以健康的动物为食时,它们可以通过唾液将病原体传播给该动物。这些疾病的早期发现和治疗对于确保受感染犬的最佳可能结果至关重要。这篇文章的目的是讨论最常见的媒介传播疾病的狗在欧洲,看看疾病的流行和健康风险的狗。我们试图总结有关疾病病理生理学,流行病学,诊断技术的优缺点,可用的治疗方法和预防方法的各个方面的最新文献。
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引用次数: 0
Detection of IgG antibodies against Borrelia burgdorferi in adults 检测成人的鲍氏包虫病 IgG 抗体
Pub Date : 2023-12-01 DOI: 10.6001/biologija.2023.69.4.2
Indrė Lipatova, Ugnė Medikaitė, D. Ambrasienė
The aim of this study was to determine the prevalence of IgG antibodies against Borrelia burgdorferi in adults who were bitten by a tick at least once and did not have classical signs and symptoms of Lyme disease. A total of 88 people from different cities in Lithuania were tested for the presence of Lyme disease IgG. The results showed that the prevalence of infection among participants was 11.4%. Unit values ranged from 12.26 U to 44.78 U. A higher number of Lyme disease cases was observed in older people and those who actively spend their time outdoors. This study shows the importance of the use of serological tests in the diagnosis of Lyme disease for patients with non-specific symptoms.
本研究的目的是确定被蜱虫叮咬至少一次且没有典型莱姆病体征和症状的成人中抗伯氏疏螺旋体IgG抗体的流行程度。立陶宛对来自不同城市的88人进行了莱姆病IgG检测。结果显示,参与者的感染率为11.4%。单位值范围为12.26 ~ 44.78 U,老年人和经常在户外活动的人群莱姆病病例较多。这项研究显示了使用血清学测试在诊断莱姆病的非特异性症状患者的重要性。
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引用次数: 0
Identification of Demodex spp. mites on human eyelashes in Lithuania 立陶宛人睫毛上的德莫德克斯属螨虫鉴定
Pub Date : 2023-12-01 DOI: 10.6001/biologija.2023.69.4.4
A. Aleksandravičienė, Rasa Volskienė, Monika Muliuolytė, Daiva Šakienė, Žaneta Maželienė
The aim of the study was to identify the Demodex mites collected from the patients’ eyelashes from the X outpatient clinic in Lithuania and the bacteria they carry. A total of 62 mites were collected from 15 different patients who complained of redness and itchiness around the eyes, rubbing around the eye area. Morphological examination of mites was carried out with an optical microscope. The Mites’ DNA was isolated using a DNA isolation kit, bacterial amplification was performed using specific primers for amplification of the 16S RNA gene fragment. Demodex folliculiorum was identified in all the examined samples, Demodex brevis was detected in only one sample. No bacteria were detected in the analysed samples.
该研究的目的是鉴定从立陶宛X门诊诊所的患者睫毛中收集的蠕形螨及其携带的细菌。从15名抱怨眼睛周围发红和发痒的不同患者中共收集了62只螨虫,摩擦眼睛周围区域。用光学显微镜对螨类进行形态学检查。采用DNA分离试剂盒分离螨体DNA,用特定引物进行细菌扩增,扩增16S RNA基因片段。所有样品均检出毛囊蠕形螨,仅1份样品检出短蠕形螨。在分析的样品中未检测到细菌。
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引用次数: 0
Anaplasma phagocytophilum in temperate and cold regions of Europe: a review on its prevalence in livestock 欧洲温带和寒冷地区嗜吞噬细胞无原体:其在牲畜中的流行情况综述
Pub Date : 2023-09-10 DOI: 10.6001/biologija.2023.69.3.4
Donata Mikalauskienė, Zoja Miknienė
In Europe, Anaplasma phagocytophilum infections in livestock have been reported in several countries, particularly in northern and central Europe, where the climate is temperate or cold. In these regions, the infection is most commonly observed in grazing animals, such as cattle and sheep, during the summer months. The prevalence of A. phagocytophilum infection in livestock can also vary within countries depending on the farming practices and management of the animals. Different studies report varying rates of infection in different countries and regions. In Europe, seroprevalence in livestock has been reported to range from 0% to 55%, with higher rates observed in regions with high tick densities. Molecular methods detect 0% to 85.71% (in animals with clinical symptoms) and 23.94% (using random selection) of A. phagocytophilum genetic material in farm animals. As the infections of Anaplasma spp. bacterium are often asymptomatic or clinical symptoms are not specific in some cases, we hypothesise that there are more anaplasmosis cases in Europe than expected. In this review we analysed scientific data excluding clinical cases, even though there are multiple cases described in different countries in the region of our review.
在欧洲,一些国家报告了牲畜中嗜吞噬细胞无原体感染,特别是在气候温和或寒冷的北欧和中欧。在这些地区,夏季感染最常见于放牧动物,如牛和羊。牲畜中嗜吞噬芽胞杆菌感染的流行程度也可能因各国的养殖方式和动物管理而异。不同的研究报告了不同国家和地区的感染率。在欧洲,据报告牲畜的血清流行率为0%至55%,在蜱虫密度高的地区观察到较高的流行率。分子方法在农场动物中检出0% ~ 85.71%(在有临床症状的动物中)和23.94%(随机选择)的嗜吞噬单胞菌遗传物质。由于无形体感染通常无症状或在某些情况下临床症状不特异性,我们假设欧洲的无形体感染病例比预期的要多。在这篇综述中,我们分析了排除临床病例的科学数据,尽管在我们的综述中,在不同的国家描述了多个病例。
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引用次数: 0
Ecotoxicological risk assessment of metalloid contamination in the sediment and benthic fauna of a tropical lotic freshwater ecosystem in Southern Nigeria 尼日利亚南部热带热带淡水生态系统沉积物和底栖动物中金属污染的生态毒理学风险评估
Pub Date : 2023-09-10 DOI: 10.6001/biologija.2023.69.3.2
Peter Osaretin Uwaifo, Nkonyeasua Kingsley Egun, Timothy Imuetinya Agho
The increasing exposure of freshwater bodies to pollutants and the bioaccumulation of heavy metals in body parts of aquatic organisms has raised concerns on the ecotoxicological and human health risk. This study evaluated the potential ecological and human health risk of heavy metal pollution in sediment and benthic fauna (Caridina africana) of the Osse River, Edo State, Nigeria. Using requisite equipment, samples of water sediment and C. africana were collected at designated stations from September 2015 to February 2017. Heavy metal concentrations in samples were determined using atomic absorption spectrophotometer. Result showed heavy metal contamination with concentration profiles of Fe > Zn > Mn > Cu > Ni > Pb > Cr > Cd > V in sediments and Fe > Zn > Mn > Cu > Ni > Cr > Pb > Cd > V in C. africana. The potential ecological risk index (PERI) values classified stations 1, 2 and 4 as of low ecological risk (PERI ≤ 150), while station 3 (PERI ≤ 300) was classified as of moderate ecological risk. Human health risk assessment for heavy metals in C. africana indicated significant non-carcinogenic health risk (HI > 1), and high carcinogenic risk to human health. The consumption of the contaminated C. africana, which is harvested in commercial quantities, portends health risk to the general public. There is need for urgent action in the abatement and regulation of identified anthropogenic activities responsible for the release of these heavy metals into the Osse River.
淡水水体越来越多地暴露于污染物以及重金属在水生生物身体部位的生物积累,引起了人们对生态毒理学和人类健康风险的关注。本研究评估了尼日利亚埃多州Osse河沉积物和底栖动物(Caridina africana)重金属污染的潜在生态和人类健康风险。2015年9月至2017年2月,利用必要的设备,在指定的站点采集了水沉积物和非洲卷虫样本。采用原子吸收分光光度计测定样品中重金属的浓度。结果表明:重金属污染表现为Fe >浓度分布图;锌比;Mn祝辞铜比;倪祝辞Pb祝辞Cr祝辞Cd比;V在沉积物和铁>锌比;Mn祝辞铜比;倪祝辞Cr祝辞Pb祝辞Cd比;C.非洲;潜在生态风险指数(PERI)将站点1、2和4划分为低生态风险(PERI≤150),而站点3 (PERI≤300)划分为中度生态风险。人类健康风险评估显示非洲古树中重金属存在显著的非致癌性健康风险(HI >1)、对人体健康有较高的致癌风险。食用受污染的商业数量的非洲香菇预示着公众的健康风险。有必要采取紧急行动,减少和管制已查明的导致这些重金属排放到奥塞河的人为活动。
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引用次数: 1
The effect of different fertilisers and planting patterns on the fatty acid profile of safflower oil 不同肥料和种植方式对红花油脂肪酸谱的影响
Pub Date : 2023-09-10 DOI: 10.6001/biologija.2023.69.3.1
Hasan Kouchakkhani, Mohsen Janmohammadi, Naser Sabaghnia
The soils of semi-arid regions have serious nutrient deficiencies, and it seems that the qualitative aspects of crops are strongly influenced by fertiliser management and planting patterns. The current experiment was carried out to evaluate the fatty acid composition of safflower oil under the application of different fertilisers (control, farmyard manure at 10 and 20 t ha–1, NPK (nitrogen, phosphorus, potassium), nano Fe+Zn) and four different planting patterns (planting in-furrow or on-ridge with inter-row distances of 40 cm or 60 cm) in the semi-arid region of north-western Iran (Maragheh). Results showed that all oil compositions significantly responded to fertiliser treatments. Utilisation of farmyard manure (FYM) increased seed oil content, and the highest oil content was recorded in planting on-ridge, which was 18% higher than the control condition. The highest percentage of seed protein was obtained by application of NPK and planting on-ridge with wider inter-row distance. Evaluation of saturated fatty acids (palmitic, stearic, myristic, lauric) showed that the application of high levels of FYM significantly increased these compositions. However, the effect of FYM20 application on main unsaturated fatty acids (linoleic acid) was much more evident, and it increased iodine value. Taken together, the results of this study indicated a positive and significant effect of the application of high levels of FYM in on-ridge planting with wider row spacing on the qualitative and nutritional aspects of safflower oil.
半干旱地区的土壤严重缺乏养分,作物的质量似乎受到肥料管理和种植方式的强烈影响。本试验旨在研究在伊朗西北部半干旱区(Maragheh)施用不同肥料(对照、10和20 t hm - 1农家肥、NPK(氮、磷、钾)、纳米铁+锌)和4种不同种植模式(沟内种植或垄上种植,行距为40 cm或60 cm)下红花油脂肪酸组成的变化。结果表明,各油分组分对施肥处理均有显著响应。施用农家肥提高了种子含油量,其中垄上种植的含油量最高,比对照高18%。施用氮磷钾和垄上种植行间距较宽,籽粒蛋白质含量最高。对饱和脂肪酸(棕榈酸、硬脂酸、肉豆蔻酸、月桂酸)的评估表明,高水平的FYM显著增加了这些成分。然而,FYM20对主要不饱和脂肪酸(亚油酸)的影响更为明显,并提高了碘值。综上所述,本研究结果表明,在宽行距的垄上种植中施用高水平FYM对红花油的质量和营养方面有积极而显著的影响。
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引用次数: 0
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Biologija
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