高温对斑点胼胝体成虫的防治效果

Arfan Ahmed Gilal
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摘要

脉冲甲虫(Callosobruchus maculatus)是世界性的脉冲粮食储藏条件下的严重害虫。考虑到合成化学品对黄斑弧菌的负面影响,在实验室条件下评估了各种高温对黄斑弧菌的效果。经典®1080系列实验室烤箱(HT公司(Ilford) Ltd.)使用英国)来获得所需的温度为40、45、50、55和60ºC。将5对新鲜出芽的黄斑锥虫成虫与20粒豇豆种子一起置于培养皿中,在每个温度条件下分别暴露10、20、30、40和50分钟。24小时后观察成人死亡率数据。结果证实了高温对黄斑弧菌成虫的有效性,在60°C条件下,10分钟内目标成虫死亡率为100%,而在55°C和50°C条件下,目标成虫死亡率分别为40和50分钟。在不同的暴露时间下,对C. maculatus所需温度的LT50值范围在暴露50分钟的43.544°C到暴露10分钟的48.994°C之间,而LT50温度的总体最小和最大基准极限范围在42.845°C到49.655°C之间。高温处理对豇豆种子的发芽率也没有负面影响,不同温度下的种子发芽率与对照没有显著差异。
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EFFECTIVENESS OF HIGH TEMPERATURES AGAINST CALLOSOBRUCHUS MACULATUS (Coleoptera: Bruchidae) ADULTS
Pulse beetle, Callosobruchus maculatus is a worldwide severe pests of pulse grains in the storage conditions. Considering the negative consequences of synthetic chemicals used against C. maculatus, effectiveness of various high temperatures were evaluated against it under lab conditions. The Classic® 1080 series laboratory oven (HT Company (Ilford) Ltd. UK) was used to get the desired temperatures of 40, 45, 50, 55, and 60ºC. Five pairs of freshly emerged C. maculatus adults were placed with twenty cowpea seeds in a petri dish  were used for each temperature regime for five exposure times i.e., 10, 20, 30, 40, and 50 minutes. Data on adult mortality was observed after 24-hours. Results confirmed the effective of high temperatures against adult C. maculatus as 100% mortality of the targeted adults was recorded within 10 minutes at 60°C, whereas it took 40- and 50-minutes to kill 100% of the adults at 55 and 50°C, respectively. The LT50 values of required temperature against C. maculatus at various exposure timings ranged between 43.544°C at 50 minutes exposure to 48.994°C at 10 minutes exposure, whereas overall minimum and maximum fiducial limits for LT50 temperatures were ranged between 42.845 to 49.655°C. No negative impact of high temperatures was also recorded on the germination percentage of cowpea seeds as germination success recorded at various high temperatures was not significantly different from the control cowpea seeds.
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