从云南山羊分离的一种强毒Akabane病毒的遗传和致病特性

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Onderstepoort Journal of Veterinary Research Pub Date : 2022-03-01 DOI:10.2478/jvetres-2022-0007
Huanan Gao, Jinping Wang, Z. Yang, Jiarui Xie, Yuwen He, Q. Hong, A. Xin
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引用次数: 2

摘要

Akabane病毒(AKAV)在中国多种宿主物种中均有发现,但在山羊中发现的记录有限。然而,更多的注意力需要放在了解该物种中病毒的多样性上。本研究旨在探讨云南地区AKAV的基因型特征、变异趋势及其与毒力的关系。材料与方法在2019年云南省山羊疾病常规监测中采集山羊血样。用BHK-21细胞分离AKAV CX-01株。为了了解病毒的致病性,将病毒腹腔和脑内接种于哺乳小鼠,并对组织样本进行组织病理学和免疫组织化学分析。结果Akabane病毒CX-01株可引起脑炎和中枢神经系统损伤,可致死亡。基于小片段ORF序列的系统发育分析表明,所使用的AKAV分离物与中国蠓类GD18134/2018和牛NM BS/1株亲缘关系最密切,而基于中片段的系统发育分析表明,CX-01与中国GLXCH01株亲缘关系最密切。结论CX-01分离株与AKAV Ia基因群有关,可能来源于不同菌株的重组。
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Genetic and Pathogenic Characterisation of a Virulent Akabane Virus Isolated from Goats in Yunnan, China
Abstract Introduction Akabane virus (AKAV) has been detected in a variety of host species in China, but there are only limited records of its occurrence in goats. However, more attention needs to be paid to understanding the diversity of viruses in this species. The aim of the study was to explore the genotype characteristics and variation trend of AKAV and their relationship with virulence in Yunnan, China. Material and Methods Blood samples were collected from goats during routine surveillance of goat diseases in Yunnan province in 2019. The AKAV CX-01 strain was isolated using BHK-21 cells. To understand pathogenicity, the virus was intraperitoneally (IP) and intracerebrally (IC) inoculated into suckling mice and tissue samples were subsequently analysed histopathologically and immunohistochemically. Results Akabane virus CX-01 strain induced encephalitis and impairment of the central nervous system with fatal consequences. Phylogenetic analysis based on the ORF sequences of the small segments indicated that the AKAV isolate used was most closely related to the GD18134/2018 Chinese midge and bovine NM BS/1strains, while phylogenetic analysis based on the medium segments showed a close relationship between CX-01 and the Chinese GLXCH01 strain. Conclusion The CX-01 isolate was related to AKAV genogroup Ia and probably originated from a recombination of different strains.
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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
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