苗前盆盘系统灵芝病易感/耐受性早期筛查研究(一)

The Planter Pub Date : 2023-05-15 DOI:10.56333/tp.2023.018
RR Lim, S. Siew, G. Hagilaa, SK Ng, TD Chew, J. Mathews
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摘要

本文提出了一种苗前盆栽系统灵芝病易感/耐受性早期筛查方法。在实验中对筛选方法的可控参数进行了测试,以便在扩大测试规模之前优化收集的数据并标准化方法。疾病的评估以外部症状为基础,包括在试验结束时进行破坏性取样以评估内部症状。从最初的实验来看,橡胶木块(RWB)接种量对锅盘尺寸的影响不显著,并选择估计的培养基接种量为4.5 x 3 x 3 cm。随后,在第二次试验中,通过将抗性和耐受性区分为明显独立的性状,筛选方法能够区分高易感和低易感后代。总的来说,盆盘筛选方法似乎足以让育种者和研究人员描述种植材料对灵芝感染的反应。关键词:灵芝病;根茎腐病;早期筛选
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Study on Early Screening of Ganoderma Disease Susceptibility/Tolerance in Pre-nursery Pot-tray System - Part 1
This paper presents a methodology for early screening of Ganoderma disease susceptibility/tolerance in pre-nursery pot-tray system. The controllable parameters for the screening method were tested in the experiment to optimise the data collected and standardise the methodology prior to broadening the scale of testing. Evaluation of the disease was based on external symptoms and included destructive sampling to assess internal symptoms at the end of the trial. From the initial experiment, the effect of the rubberwood block (RWB) inoculum size was considered to be not significant in relation to the pot-tray size and the estimated medium inoculum size of 4.5 x 3 x 3 cm was selected. Subsequently, during the second experiment, through differentiation of resistance and tolerance as distinctly separate traits, the screening method was able to distinguish highly susceptible and less susceptible progenies. Overall, the pot-tray screening method seems adequate in allowing breeders and researchers to characterise the planting material’s response towards Ganoderma infection. Keywords: Ganoderma disease, basal stem rot, early screening, pre-nursery, pot-tray
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