通过涉及乳糖、苹果酸和柠檬酸的反应产生益生元

J. Csapó, D. Kiss, C. Albert
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引用次数: 0

摘要

益生元是一种不易消化的食物成分,它进入结肠,为双歧杆菌和乳酸菌提供营养。由于纤维和低聚糖是典型的益生元,我们在实验中通过乳糖和苹果酸以及柠檬酸的反应产生益生元,这些反应使用了适当浓度的这些物质,有足够的持续时间,并在最佳温度条件下进行。我们确定了反应的最佳参数,测定了原料的损失和最终产物浓度的增加,并分析了盐酸水解后益生元的总糖含量。体外实验证明了我们的最终产品对碳水化合物降解酶的抵抗力,这是益生元的基本要求,因此当它到达结肠时,它可以作为益生菌的营养物质。
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Production of prebiotics via reactions involving lactose as well as malic acid and citric acid
Abstract Prebiotics are such indigestible food ingredients that enter the colon and serve as nutrient for bifidobacteria and lactobacilli. Since fibres and oligosaccharides are the typical prebiotics, we produced prebiotics in our experiments with the reaction of lactose and malic acid as well as citric acid, where these reactions made use of an appropriate concentration of these substances, had an adequate duration, and were carried out under optimal temperature conditions. We determined the optimal parameters of the reaction, measured the loss of the starting materials as well as the increase in concentration of the end-product, and analysed the total sugar content of the hydrolysed prebiotics after hydrolysis by hydrochloric acid. In vitro experiments were performed to demonstrate our end-product’s resistance to carbohydrate-degrading enzymes, which is a fundamental requirement for a prebiotic so that upon reaching the colon it can serve as nutrient for the probiotic bacteria found there.
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