Association Of Il18 Gene Polymorphism with Susceptibility to Periodontitis - A Case Control Study

Background: Cytokine IL18, also known as IFN-inducing factor, is an important regulator of innate and acquired immune responses and plays a number of roles in chronic inflammation and dysfunction autoimmune disorder. Several polymorphisms in the IL18 promoter region have been identified. As there is little evidence for IL18 polymorphisms and periodontitis, this study aimed to evaluate the genetic association of polymorphisms of the IL18 gene (rs1946518). Materials and Methods: A total of 100 subjects were recruited for this study, including 50 patients with periodontitis (stage II, III and grade B) and 50 patients with healthy gingiva clinically intact periodontium. Genomic DNA was extracted from the blood obtained from the subjects. DNA was amplified using primers specific to the MseI region of the IL18 receptor gene. Amplicon was further genotyped using the restriction fragment length using the BtgI enzyme. Genotyping obtained on the basis of the RFLP model was recorded and used for statistical analysis. The distributions of genotypes and allele frequencies in the periodontal disease group and the control group were compared using the Chi Square test. Risks associated with individual alleles or genotypes were calculated using odds ratios (ORs) with 95% confidence intervals. Statistical significance in all trials was determined at p <0.05. Result: The frequencies of genotypes and the distribution of IL18 receptor MseI polymorphisms were not significantly different at ꭕ2df (P = .818). The results of our study showed that there was no significant difference between homozygous and heterozygous genotypes (GG vs TG TT) between periodontitis patients and control group with a P value of 0.8316. The detection frequencies of the TG (20% vs 22%) and TT (14% vs 10%) genotypes showed a significant difference (P value = 0.8316) between the control and experimental groups. experience. There were no significant differences for the G allele (76% vs 79%) and the T allele (24% vs 21%) between the experimental and control groups. Conclusions: The present study indicated that the polymorphism of the IL18 gene was not associated with CP in the analyzed study group.