非产毒曲霉防治甘蔗红腐病的研究。

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Chiang Mai Journal of Science Pub Date : 2023-05-31 DOI:10.12982/cmjs.2023.032
Atcharaporn Srijudanu, O. Piasai, W. Leesutthiphonchai, Mattana Wanitch, C. Chaisiri, N. Khewkhom
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引用次数: 0

摘要

由镰状炭疽菌引起的红腐病是泰国几种甘蔗品种的主要生产威胁。生物防治是利用微生物拮抗作用和相关代谢产物管理疾病的一种生态友好的替代方法。本研究采用遗传方法对4株非产毒曲霉菌株(A37、A374、A400和A561)进行了鉴定,并对其抑制镰状镰刀菌生长的拮抗潜力进行了评价。菌株A561的基因缺失数量最多,丢失了12个产生毒素的基因。此外,采用AFB1酶联免疫吸附试验证实了A561的非毒素特性。与产毒对照相比,A561使AFB1浓度降低了98.57% (1.63 μg/kg)。采用马铃薯葡萄糖琼脂(PDA)竞争平板法测定了A561对镰刀镰刀菌的体外拮抗活性。结果表明,A561对镰刀霉菌丝生长的抑制作用为34.39%。此外,10% (v/v)的A561在PDA上的培养滤液对菌丝的抑制率为53.21%。非产毒的A561具有抑菌潜能,能抑制镰状镰刀菌的生长。
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Biocontrol of Colletotrichum falcatum Causing Red Rot Disease of Sugarcane Using Non-toxigenic Aspergillus sp.
Red rot disease caused by Colletotrichum falcatum is a major production threat to several sugarcane varieties in Thailand. Biological control is an ecologically friendly alternative to manage the disease using microbial antagonism and associated metabolic products. In this study, four Aspergillus non-toxigenic strains (A37, A374, A400, and A561) were characterized using a genetic approach and evaluated for their antagonistic potential to suppress the growth of C. falcatum. The strain A561 had the maximum number of gene deletions, with 12 afl atoxin-producing genes being lost. In addition, the non-toxigenic characteristic of A561 was confi rmed using afl atoxin B1 (AFB1) enzyme-linked immunosorbent assay. A561 reduced the concentration of AFB1 by 98.57% (1.63 μg/kg) relative to the toxigenic control. The antagonistic activity of A561 was evaluated against C. falcatum using the competition plate method on potato dextrose agar (PDA) in vitro. The results revealed that A561 inhibited the mycelial growth of C. falcatum by 34.39%. In addition, 10% (v/v) culture fi ltrate of A561 on PDA produced mycelial inhibition of 53.21%. Non-toxigenic A561 had antifungal potential, suppressing the growth of C. falcatum.
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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