菲律宾不同竹种竹妖帚病的分布及“候选植物原体”相关菌株16SrVIII的分子鉴定

Q3 Multidisciplinary Philippine Journal of Science Pub Date : 2023-07-03 DOI:10.56899/152.04.13
L. Dolores, Joanne A. Langres, Cris Q. Cortaga, M. CAASI-LIT
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引用次数: 0

摘要

在菲律宾,竹子被用来制作建筑、家具、手工艺品和绳索,也是一种食物来源。该国竹子的新发疾病之一是竹帚病(BWB),自20世纪90年代以来,在菲律宾各省(北伊洛科斯省、拉古纳省、巴丹加斯省、奎松省、南阿古桑省、布基农省和南哥打巴托省)的三个竹属中偶尔发现了这种疾病,包括Dendrocalamus、Gigantochloa和Schizostachyum。然而,该国关于生物多样性的研究和信息仍然缺乏,而且在很大程度上尚未开发。在这项研究中,我们报告了一种类似的疾病,影响了保和岛和达沃的竹和竹,并首次在伊莎贝拉和新比斯开的梅花竹("bayog")和竹("kawayan-tinik")中发生。根据1999年至2019年进行的调查,目前在菲律宾11个省记录的4个属的6个竹种中发现了该病,即Gigantochloa spp. (G. levis和G. ter)、Dendrocalamus spp. (D. aspper和D. merrillianus)、Schizostachyum lumampao和Bambusa spinosa。BWB症状包括叶片聚集形成玫瑰状结构,叶片增生,单个节点形成过多的肢体,节间缩短。采用通用引物P1/P7和R16MF2/R1对来自菲律宾伊莎贝拉的5份症状性BWB样本进行巢式PCR检测,结果显示扩增阳性。对伊莎贝拉代表性BWB菌株进行测序(~ 1.3kbp)和系统发育分析,结果表明该菌株与丝瓜候选菌(Candidatus Phytoplasma luffae)的遗传相似性和聚类性> 98.65%,属16SrVIII类群(Loofah Witches’Broom group)。本文确定了BWB在菲律宾不同竹种中的分布,以及“Ca. Phytoplasma luffae”相关菌株(16SrVIII)与BWB的关联。
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Distribution of Bamboo Witches’ Broom Disease in Various Bamboo Species in the Philippines and Molecular Identification of 'Candidatus Phytoplasma luffae'-related Strain 16SrVIII
Bamboo is used for making structures, furniture, handicrafts, and ropes, as well as a source of food in the Philippines. One of the emerging diseases of bamboo in the country is the bamboo witches’ broom (BWB), which has been occasionally noted in three genera of bamboo – including Dendrocalamus, Gigantochloa, and Schizostachyum from various provinces in the Philippines (Ilocos Norte, Laguna, Batangas, Quezon, Agusan del Sur, Bukidnon, and South Cotabato) since the 1990s. However, studies and information about BWB in the country remain lacking and largely unexplored. In this study, we report a similar disease affecting Dendrocalamus and Gigantochloa bamboo species from Bohol and Davao and – for the first time – in Dendrocalamus merrillianus ("bayog") and Bambusa spinosa ("kawayan-tinik") from Isabela and Nueva Vizcaya. As a result of the surveys conducted from 1999–2019, the disease is now identified in six species across four genera of bamboo – namely, Gigantochloa spp. (G. levis and G. atter), Dendrocalamus spp. (D. asper and D. merrillianus), Schizostachyum lumampao, and Bambusa spinosa recorded in 11 provinces in the Philippines. The BWB symptoms include clustering of leaves forming a rosette-like structure, leaf proliferation, excessive limb formation from a single node, and shortening of internodes. Nested PCR using the universal primers P1/P7 and R16MF2/R1 targeting the phytoplasma 16S ribosomal RNA gene revealed positive amplification in five symptomatic BWB samples from Isabela, Philippines. Subsequent sequencing (~ 1.3kbp) and phylogenetic analysis using the representative BWB isolates from Isabela revealed > 98.65% genetic similarity and clustering to Candidatus Phytoplasma luffae, which belongs to the 16SrVIII group (Loofah Witches’ Broom Group). This paper determined the distribution of BWB in different species of bamboo in the Philippines, as well as the association of 'Ca. Phytoplasma luffae'-related strain (16SrVIII) to BWB.
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来源期刊
Philippine Journal of Science
Philippine Journal of Science Multidisciplinary-Multidisciplinary
CiteScore
1.20
自引率
0.00%
发文量
55
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