In vitro Propagation of Anthurium andraeanum Linn. (White) via Indirect Organogenesis through the Use of Leaf Lamina and Petiole Explants

This research discribes an efficient protocol for the micropropagation of Anthurium andraeanum Linn. with the use of plant growth regulators namely; 2,4-D and BAP through indirect organogenesis of callus formation. Callus induction, callus proliferation, shoot initiation, shoot multiplication and root formation from regenerated shoots were investigated during this research. The multiple segments of youngest and newest leaf laminas and petioles from the adult plant were used as explants in current experiment. Both explant segments gave regenerated plantlets when they were exposed to plant hormones previously mentioned in basal MS full strength medium. The highest callus formation was found in MS medium supplemented with 6mg/L 2,4-D among other concentrations of 2,4-D such as 0.4, 0.5, 0.6, 0.7 and 0.8 mg/L. For multiplication purpose, BAP 3mg/L was the best among other treatments (BAP 1, 1.5, 2, 2.5 mg/L) in the same basal MS medium. Leaving well-developed shoots in the same medium without changing into the fresh medium caused rooting from these shoots. After about three months, the regenerated Anthurium plantlets were successfully transferred to clay pots containing the ratio of (3:2:1) coconut husks, broken bricks and charcoals.