河流州立大学宿舍女学生浴巾细菌的特征与鉴定

C. Ugboma, H. C. Otamiri
{"title":"河流州立大学宿舍女学生浴巾细菌的特征与鉴定","authors":"C. Ugboma, H. C. Otamiri","doi":"10.9734/mrji/2022/v32i101346","DOIUrl":null,"url":null,"abstract":"Bath towels are an important part of our everyday life, but they often amass a large number of micro-organisms which may sometimes be harmful to us. For the study a total of 10 samples plus a control were collected from bath towels of female students in NDDC hostel, post-graduate hostel and control from Mile 3 market respectively between November 2021 to February 2022. Standard methods were employed for the sampling and determination of microbiological characteristics. Identification of bacteria was carried out using colonial, morphological and biochemical characteristics. Statistical analysis were performed using the T-test method and sensitivity was carried out on the isolates to detect pathogenicity. Range of microbial counts of NDDC hostel were: Total heterotrophic bacteria 2.0 x 104 cfu/ml to 3.17 x 104 cfu/ml, Total coliform count 0.48 x 104 cfu/ml to 2.99 x 104 cfu/ml in the first sampling. Post graduate hostel ranged from: Total heterotrophic bacteria 1.48 x 104 cfu/ml to 0.18 x 104 cfu/ml in the first sampling. For the second sampling, NDDC hostel microbial count ranged from 0.73 x 104 cfu/l to 1.15 x 104 cfu/ml in the total heterotrophic bacteria, and 0.31 x 104 cfu/ml to 0.13 x 104 cfu/ml for total coliform count. Post graduate hostel ranged from 0.27 x 104 cfu/ml to 0.93 x 104 cfu/ml for total heterotrophic bacteria and 0.22 x 104 cfu/ml to 0.18 x 104 cfu/ml for total coliform count. The control microbial count for total heterotrophic bacteria were 0.12 x 104 and 0.16 x 104 cfu/ml for the first and second sampling respectively and for the total coliform count, 0.18 x 104 cfu/ml and 0.17 x 104 cfu/ml for the first and second sampling respectively. Statistical analysis using student’s T-test was carried out. The mean test values for total heterotrophic bacteria in the first and second samplings were 2.2750E2 and 57.4000 in NDDC hostel and 1.5060E2 and 66.8000 in post graduate hostel. The mean values for total coliform count for the first and second samplings were 1.6220E2 and 24.5000 in NDDC hostel and 99.8000 and 26.9000 in Post graduate hostel. The test revealed that there was no significant difference in the bacterial load of NDDC hostel and Post graduate hostel from the two samplings. In the antimicrobial susceptibility test carried out, results showed that Ciproflox, Reflacine and Tarrivid were most effective against the isolates, whileth the isolates mostly showed resistance against Amplicox, Amoxil and Azithromycin. The bacteria species isolated were Staphylococcus aureus, Escherichia coli, Staphylococcus spp, Bacillus cereus, Klebsiella spp, Bacillus spp. The study demonstrated that significant numbers of E.coli, and staphylococcus as well as other microbes occur in bath towels.","PeriodicalId":18450,"journal":{"name":"Microbiology Research Journal International","volume":"78 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization and Identification of Bacteria Present in the Bath Towels of Female Students in Rivers State University Hostels\",\"authors\":\"C. Ugboma, H. C. Otamiri\",\"doi\":\"10.9734/mrji/2022/v32i101346\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bath towels are an important part of our everyday life, but they often amass a large number of micro-organisms which may sometimes be harmful to us. For the study a total of 10 samples plus a control were collected from bath towels of female students in NDDC hostel, post-graduate hostel and control from Mile 3 market respectively between November 2021 to February 2022. Standard methods were employed for the sampling and determination of microbiological characteristics. Identification of bacteria was carried out using colonial, morphological and biochemical characteristics. Statistical analysis were performed using the T-test method and sensitivity was carried out on the isolates to detect pathogenicity. Range of microbial counts of NDDC hostel were: Total heterotrophic bacteria 2.0 x 104 cfu/ml to 3.17 x 104 cfu/ml, Total coliform count 0.48 x 104 cfu/ml to 2.99 x 104 cfu/ml in the first sampling. Post graduate hostel ranged from: Total heterotrophic bacteria 1.48 x 104 cfu/ml to 0.18 x 104 cfu/ml in the first sampling. For the second sampling, NDDC hostel microbial count ranged from 0.73 x 104 cfu/l to 1.15 x 104 cfu/ml in the total heterotrophic bacteria, and 0.31 x 104 cfu/ml to 0.13 x 104 cfu/ml for total coliform count. Post graduate hostel ranged from 0.27 x 104 cfu/ml to 0.93 x 104 cfu/ml for total heterotrophic bacteria and 0.22 x 104 cfu/ml to 0.18 x 104 cfu/ml for total coliform count. The control microbial count for total heterotrophic bacteria were 0.12 x 104 and 0.16 x 104 cfu/ml for the first and second sampling respectively and for the total coliform count, 0.18 x 104 cfu/ml and 0.17 x 104 cfu/ml for the first and second sampling respectively. Statistical analysis using student’s T-test was carried out. The mean test values for total heterotrophic bacteria in the first and second samplings were 2.2750E2 and 57.4000 in NDDC hostel and 1.5060E2 and 66.8000 in post graduate hostel. The mean values for total coliform count for the first and second samplings were 1.6220E2 and 24.5000 in NDDC hostel and 99.8000 and 26.9000 in Post graduate hostel. The test revealed that there was no significant difference in the bacterial load of NDDC hostel and Post graduate hostel from the two samplings. In the antimicrobial susceptibility test carried out, results showed that Ciproflox, Reflacine and Tarrivid were most effective against the isolates, whileth the isolates mostly showed resistance against Amplicox, Amoxil and Azithromycin. The bacteria species isolated were Staphylococcus aureus, Escherichia coli, Staphylococcus spp, Bacillus cereus, Klebsiella spp, Bacillus spp. The study demonstrated that significant numbers of E.coli, and staphylococcus as well as other microbes occur in bath towels.\",\"PeriodicalId\":18450,\"journal\":{\"name\":\"Microbiology Research Journal International\",\"volume\":\"78 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology Research Journal International\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/mrji/2022/v32i101346\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Research Journal International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/mrji/2022/v32i101346","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

浴巾是我们日常生活的重要组成部分,但它们往往聚集了大量的微生物,有时可能对我们有害。本研究于2021年11月至2022年2月期间分别从ndc宿舍、研究生宿舍和Mile 3市场的女学生浴巾中收集了10个样本和一个对照。采用标准方法取样和测定微生物特性。利用菌落、形态和生化特征对细菌进行鉴定。采用t检验方法进行统计分析,并对分离株进行敏感性检测致病性。NDDC旅舍微生物计数范围为:异养菌总数2.0 × 104 cfu/ml ~ 3.17 × 104 cfu/ml,大肠菌群总数0.48 × 104 cfu/ml ~ 2.99 × 104 cfu/ml。研究生宿舍的异养细菌总数从1.48 × 104 cfu/ml到0.18 × 104 cfu/ml不等。在第二次采样中,NDDC宿舍微生物总数在总异养细菌中为0.73 × 104 cfu/l至1.15 × 104 cfu/ml,总大肠菌群计数为0.31 × 104 cfu/ml至0.13 × 104 cfu/ml。研究生宿舍的总异养菌数为0.27 × 104 cfu/ml至0.93 × 104 cfu/ml,大肠菌群总数为0.22 × 104 cfu/ml至0.18 × 104 cfu/ml。第一次和第二次取样的对照微生物总数分别为0.12 × 104和0.16 × 104 cfu/ml,第一次和第二次取样的总大肠菌群计数分别为0.18 × 104 cfu/ml和0.17 × 104 cfu/ml。采用学生t检验进行统计分析。第一次和第二次样本总异养细菌的平均检测值分别为:NDDC宿舍为2.2750E2和57.4000,研究生宿舍为1.5060E2和66.8000。第一次和第二次抽样的大肠菌群总数平均值分别为:NDDC宿舍的1.6220E2和24.5000,研究生宿舍的99.8000和26.9000。结果表明,两个样品的NDDC宿舍和研究生宿舍的细菌负荷无显著差异。药敏试验结果显示,环丙洛芬、雷伐平和他利伟对分离株的抗菌效果最好,而分离株对Amplicox、阿莫西和阿奇霉素的耐药程度最高。分离到的细菌种类有金黄色葡萄球菌、大肠杆菌、葡萄球菌、蜡样芽孢杆菌、克雷伯氏杆菌、芽孢杆菌等。研究表明,浴巾中存在大量的大肠杆菌和葡萄球菌等微生物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Characterization and Identification of Bacteria Present in the Bath Towels of Female Students in Rivers State University Hostels
Bath towels are an important part of our everyday life, but they often amass a large number of micro-organisms which may sometimes be harmful to us. For the study a total of 10 samples plus a control were collected from bath towels of female students in NDDC hostel, post-graduate hostel and control from Mile 3 market respectively between November 2021 to February 2022. Standard methods were employed for the sampling and determination of microbiological characteristics. Identification of bacteria was carried out using colonial, morphological and biochemical characteristics. Statistical analysis were performed using the T-test method and sensitivity was carried out on the isolates to detect pathogenicity. Range of microbial counts of NDDC hostel were: Total heterotrophic bacteria 2.0 x 104 cfu/ml to 3.17 x 104 cfu/ml, Total coliform count 0.48 x 104 cfu/ml to 2.99 x 104 cfu/ml in the first sampling. Post graduate hostel ranged from: Total heterotrophic bacteria 1.48 x 104 cfu/ml to 0.18 x 104 cfu/ml in the first sampling. For the second sampling, NDDC hostel microbial count ranged from 0.73 x 104 cfu/l to 1.15 x 104 cfu/ml in the total heterotrophic bacteria, and 0.31 x 104 cfu/ml to 0.13 x 104 cfu/ml for total coliform count. Post graduate hostel ranged from 0.27 x 104 cfu/ml to 0.93 x 104 cfu/ml for total heterotrophic bacteria and 0.22 x 104 cfu/ml to 0.18 x 104 cfu/ml for total coliform count. The control microbial count for total heterotrophic bacteria were 0.12 x 104 and 0.16 x 104 cfu/ml for the first and second sampling respectively and for the total coliform count, 0.18 x 104 cfu/ml and 0.17 x 104 cfu/ml for the first and second sampling respectively. Statistical analysis using student’s T-test was carried out. The mean test values for total heterotrophic bacteria in the first and second samplings were 2.2750E2 and 57.4000 in NDDC hostel and 1.5060E2 and 66.8000 in post graduate hostel. The mean values for total coliform count for the first and second samplings were 1.6220E2 and 24.5000 in NDDC hostel and 99.8000 and 26.9000 in Post graduate hostel. The test revealed that there was no significant difference in the bacterial load of NDDC hostel and Post graduate hostel from the two samplings. In the antimicrobial susceptibility test carried out, results showed that Ciproflox, Reflacine and Tarrivid were most effective against the isolates, whileth the isolates mostly showed resistance against Amplicox, Amoxil and Azithromycin. The bacteria species isolated were Staphylococcus aureus, Escherichia coli, Staphylococcus spp, Bacillus cereus, Klebsiella spp, Bacillus spp. The study demonstrated that significant numbers of E.coli, and staphylococcus as well as other microbes occur in bath towels.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Assessing the Microbial Burden on Hostel Bed Linens: A Threat to Student Health Detection of Carbapenemases in Pseudomonas aeruginosa Isolates: An Emerging Challenge Isolation and Characterization of Staphylococcus saprophyticus from Textile Industry Soil for Decolorization of Crystal Violet Dye Morphological Exploration of Lignicolous Macrofungi: Xylaria Gut Microbiota and Estrogen Levels in Women Breast Cancer in Côte d'Ivoire
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1