Mst Jasmin Akter, Sekendar Ali, Iffat Hasnin, N. A. Khatune, A. Rahman
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引用次数: 2
摘要
本研究旨在通过盐水对虾致死生物测定和孔交叉、空地和升高+迷宫(EPM)实验对瑞士白化病小鼠的镇静作用进行评价。在盐水对虾实验中,CME、pet-醚(PETF)、氯仿(CHF)和水溶液(AQF)的LC50分别为110、24.55、85和14.45 μg/ml,与标准硫酸长春新碱(7.5 μg/ml)相比,PETF和AQF具有显著的细胞毒性。在野外试验中,对照、标准安定、CME、CHF和AQF的运动次数分别为40.90次、12.43次、5.45次、3.66次和29.74次/ min (120 min后),表明氯仿可溶性部位的活性较其他样品强。在孔交叉试验中也观察到类似的数据,120 min后标准、CME、CHF和AQF的每分钟运动次数分别为3.60次、5.56次、4.77次和7.99次。在EPM测试中,CME显示小鼠进入EPM张开臂的百分比以及在张开臂中花费的时间百分比显着降低。结果表明,CHF对运动活性的抑制作用明显优于甲醇和水溶液组分。这些发现将有助于生物测定指导分离负责细胞毒性和镇静活性的活性成分。孟加拉药学杂志24(2):105-110,2021
Cytotoxicity and Sedative Activity of Steam Bark of Dillenia indica L.
The study aims to assess the cytotoxic activity of the methanolic extract (CME) and various fractions from the bark of Dillenia indica L (Family: Dilleniaceae) using brine shrimp lethality bioassay and sedative activities using hole cross, open field, and elevated-plus maze (EPM) test in Swiss albino mice. In brine shrimp assay, the LC50 for CME, pet-ether (PETF), chloroform (CHF) and aqueous (AQF) fraction were found to be 110, 24.55, 85 and 14.45 μg/ml, respectively, indicating significant cytotoxicity of PETF and AQF when compared to the standard vincristine sulfate (7.5 μg/ml). In open field test, the number of movements per min (after 120 min) was 40.90, 12.43, 5.45, 3.66, and 29.74 for control, standard diazepam, CME, CHF and AQF, respectively, indicating strong activity of the chloroform soluble fraction compared to the other test samples of D. indica. Similar data was observed in hole cross test where the number of movements per minute after 120 min were 3.60, 5.56, 4.77, and 7.99 for the standard, CME, CHF and AQF, respectively. In EPM test, the CME showed a significant decreased percentage of entries of mice into the open arms of EPM as well as the percentage of time spent in the open arms. The result indicates that the CHF significantly suppressed the locomotor activity than that of methanol and aqueous fractions. These findings will be helpful for bioassay-guided isolation of active principles responsible for cytotoxicity and sedative activities.
Bangladesh Pharmaceutical Journal 24(2): 105-110, 2021