{"title":"高灵敏度n -(9-吖啶基)马来酰亚胺(nam)荧光测定法测定环境和生物样品中低于PPM水平的亚硫酸盐","authors":"H. Meguro, K. Akasaka, H. Ohrui, S. Matsui","doi":"10.1081/TMA-120015608","DOIUrl":null,"url":null,"abstract":"ABSTRACT A highly sensitive fluorometric determination of sulfite in biological and environmental samples is described. Sulfite reacted with N-(9-acridinyl)maleimide (NAM), a Michael addition reaction, to give strongly fluorescent derivatives at temperatures ranging from room temperature to 50°C. There was no interference by the inorganic salts, cations and anions nor by the reducing, oxidizing and organic substances present in wine, beer and blood serum. The sensitivities were in the µM range in the batch methods and in the pmol range when using an ODS column in high performance liquid chromatograpy (HPLC).","PeriodicalId":17525,"journal":{"name":"Journal of Trace and Microprobe Techniques","volume":"27 1","pages":"463 - 472"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"HIGHLY SENSITIVE N-(9-ACRIDINYL)MALEIMIDE (NAM) FLUOROMETRY TO DETERMINE SULFITE BELOW PPM LEVEL IN ENVIRONMENTAL AND BIOLOGICAL SAMPLES\",\"authors\":\"H. Meguro, K. Akasaka, H. Ohrui, S. Matsui\",\"doi\":\"10.1081/TMA-120015608\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT A highly sensitive fluorometric determination of sulfite in biological and environmental samples is described. Sulfite reacted with N-(9-acridinyl)maleimide (NAM), a Michael addition reaction, to give strongly fluorescent derivatives at temperatures ranging from room temperature to 50°C. There was no interference by the inorganic salts, cations and anions nor by the reducing, oxidizing and organic substances present in wine, beer and blood serum. The sensitivities were in the µM range in the batch methods and in the pmol range when using an ODS column in high performance liquid chromatograpy (HPLC).\",\"PeriodicalId\":17525,\"journal\":{\"name\":\"Journal of Trace and Microprobe Techniques\",\"volume\":\"27 1\",\"pages\":\"463 - 472\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Trace and Microprobe Techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1081/TMA-120015608\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Trace and Microprobe Techniques","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1081/TMA-120015608","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
HIGHLY SENSITIVE N-(9-ACRIDINYL)MALEIMIDE (NAM) FLUOROMETRY TO DETERMINE SULFITE BELOW PPM LEVEL IN ENVIRONMENTAL AND BIOLOGICAL SAMPLES
ABSTRACT A highly sensitive fluorometric determination of sulfite in biological and environmental samples is described. Sulfite reacted with N-(9-acridinyl)maleimide (NAM), a Michael addition reaction, to give strongly fluorescent derivatives at temperatures ranging from room temperature to 50°C. There was no interference by the inorganic salts, cations and anions nor by the reducing, oxidizing and organic substances present in wine, beer and blood serum. The sensitivities were in the µM range in the batch methods and in the pmol range when using an ODS column in high performance liquid chromatograpy (HPLC).
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