埃及北京商品鸭养殖场禽白血病病毒J亚群的检测

W. Kilany, M. Soliman, Marwa M Safwat, O. Mehana, M. A. Magid, Mohamed K. Hassan, S. Nasif
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引用次数: 2

摘要

禽白血病病毒J亚群(ALV-J)在肉鸡和蛋鸡中广泛存在,但在鸭中很少观察到。在本研究中,在埃及饲养的两群北京鸭死亡率为25-30%,产蛋率下降20-30%,孵化率下降60-65%。大体图显示肝脏、脾脏严重肿大,心脏白色隆起结节,卵巢萎缩。肝、脾表面及切面均可见弥漫性多灶白色凸起灶。组织病理学检查显示心、肝、肾、卵巢内浸润大量体积较大的髓细胞,外周核大,胞质颗粒呈红色。部分髓细胞有有丝分裂象。结果抗原捕获ELISA法检测ALV抗原p27阳性。PCR结果表明,禽群ALV-J阳性,特异性片段为545 bp, ALV-A、马立克病病毒(MDV)和网状内皮增生病毒(REV)阴性。本研究为ALV-J诱导鸭髓细胞瘤病提供了一些信息。结论是ALV-J病毒正在扩大宿主范围,包括鸭子。此外,骨髓性白血病是家禽业面临的一个长期问题。
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Detection of Avian Leukosis Virus Subgroup J from Commercial Peking Duck Breeder Farm in Egypt
Avian Leukosis Virus subgroup J (ALV-J) is widely described in meat-type chickens and layers type but rarely observed in ducks. In this study, two flocks of Peking duck breeder bred in Egypt showed 25-30% mortality, 20-30% drop in egg production and 60-65% drop in hatchability. Gross picture showed severe enlargement of liver, spleen, white raised nodules in heart and ovarian atrophy in all examined birds. The liver and spleen had diffuse, multifocal white raised foci on the surface as well as on the cut-surface. Histopathological examination revealed numerous myelocytes with bigger volume, large peripheral nucleus and packed reddish cytoplasmic granules infiltrated in heart, liver, kidney and ovary. Some of myelocytic cells had mitotic figures. Results were positive for detection of ALV antigenp27 by antigen capture ELISA in cloacal samples. The PCR results confirmed that the flocks were positive for ALV-J with specific fragment of 545 bp, but negative for ALV-A, Marek’s Disease Virus (MDV) and Reticuloendotheliosis virus (REV). The study provided some information on ALV-J induced myelocytomatosis for ducks. It concluded that ALV-J virus is broadening host range including the ducks. Also, myeloid leukosis is an enduring problem facing the poultry industry.
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