黑曲霉深层发酵产柠檬酸的研究

Meva Gustina E. Sidauruk, Surya Ningsih Hutauruk, M. M. Martgrita, A. Manurung
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摘要

多巴香蕉皮废弃物来源于多巴香蕉果实(Musa acuminata Colla)加工。当地人通常把香蕉皮废料用作牲畜饲料。如果处理不当,垃圾也会造成环境问题。香蕉皮废料的碳水化合物含量很高,可以发酵产生更有价值的产品,其中一种是柠檬酸。柠檬酸是一种有机酸,在全球范围内被大量消费和生产。在食品和饮料工业中,柠檬酸因其溶解度高、无毒、口感好等特点,被广泛用于各种用途。本研究的目的是确定利用黑曲霉对香蕉皮进行深层发酵生产柠檬酸的最佳条件。不同浓度的香蕉皮(5%、10%和15% w/v)加5%蔗糖或5%葡萄糖(w/v)处理。在发酵过程中,测量pH值,以确定pH值的变化表明柠檬酸的生产。结果表明,香蕉皮底物浓度和糖种类的变化对柠檬酸产量有影响。黑曲霉深层发酵的最佳条件为:底物浓度为15%,添加5%蔗糖,柠檬酸产量为0.651% (w/v)。
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Citric Acid Production from Toba Banana Peel (Musa acuminata Colla) through Submerged Fermentation using Aspergillus niger
Toba banana peel waste is derived from Toba banana fruit (Musa acuminata Colla) processing. Local people utilized banana peel waste usually as livestock feed. The waste also can make an environmental problem if it is not handling well. Banana peel waste has a high content of carbohydrate that can be fermented to produce a more valuable product, one of which is citric acid. Citric acid is an organic acid that is consumed globally and produced in large quantities. In food and beverages industries, citric acid is used for various purposes due to its high solubility, non-toxic and good taste characteristics. The objective of this research is to determine the optimum conditions of submerged fermentation of banana peel to produce citric acid using Aspergillus niger. The treatments were various banana peel concentrations (5%, 10% and 15% w/v) added with 5% sucrose or 5% glucose (w/v). During the fermentation, pH was measured to determine pH changes indicated the production of citric acid. The results showed that the variation concentration of banana peel substrate and type of sugars affect citric acid production. The optimum condition of submerged fermentation by Aspergillus niger was obtained at 15% substrate concentration by adding 5% sucrose to produce 0.651% (w/v) of citric acid.
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