评价藏红花素对双酚a诱导的大鼠记忆损伤的作用:海马ERK、CaMKII和CREB蛋白的作用

Zeinab Bedrood, Elaheh Masjedi, F. Hassani, M. Rahbardar, Hossein Hosseinzadeh, Khalil Abnous, S. Mehri, دوردب بنیز, یدجسم ههلا
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摘要

简介:双酚A (BPA)是一种有毒的化学物质,在塑料产品的生产中被用作单体。BPA具有干扰内分泌的作用,并对神经系统造成损害。藏红花素是藏红花中的一种有效成分,具有神经保护作用。因此,本研究旨在评估藏红花素对大鼠bpa诱导的记忆障碍的影响,以及海马细胞外信号调节激酶(ERK)、Ca2+/钙调素依赖性蛋白激酶ii (CaMKII)和环amp反应元件结合蛋白(CREB)蛋白的水平。方法:双酚A (100 mg/kg,灌胃)和藏红花素(10、20、40 mg/kg,灌胃)灌胃28 d。Morris水迷宫法评价大鼠的记忆和学习功能,Western blot法测定蛋白质水平。结果:与对照组相比,BPA组(100 mg/kg)显著诱导记忆损伤,并降低了ERK、p.ERK、CaMKII和p.CREB蛋白的水平。另一方面,与BPA (100 mg/kg)组相比,藏红花素(20 mg/kg)显著改善了学习和记忆障碍,并增加了海马中ERK、p.r ERK和CaMKII蛋白的水平。结论:藏红花素(20 mg/kg)可改善BPA (100 mg/kg)所致的记忆和学习缺陷。增强的_ p。海马中ERK、ERK和p. CaMKII蛋白水平在藏红花素保护作用中起重要作用
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Evaluation the Effect of Crocin on Bisphenol A-Induced Memory Impairment in Rats: Role of ERK, CaMKII, and CREB Proteins in Hippocampus
Introduction: Bisphenol A (BPA) is a toxic chemical that is used as a monomer in the production of plastic products. The BPA has an endocrine-disrupting effect and ‎induces damage to the nervous system. Crocin, an active ingredient of Crocus sativus L. has ‎neuroprotective effects. Therefore, this study aimed to evaluate crocin’s effects on BPA-induced ‎memory impairment in rats and the levels of Extracellular Signal-Regulated Kinases (ERK), Ca2+/Calmodulin-Dependent Protein Kinase-II (CaMKII), and Cyclic AMP-Response Element-Binding Protein (CREB) proteins in the hippocampus. Method: Bisphenol A (100 mg/kg, gavage) and crocin (10, 20, and 40 mg/kg, IP) were administered to adult male rats for 28 days. Memory and learning functions were evaluated by the Morris water maze, and the level of proteins was determined using the Western blot method. Results: Administration of the BPA (100 mg/kg) significantly induced memory impairment and reduced the levels of ERK, p.ERK, CaMKII, and p.CREB proteins in the BPA group, compared to the control group. On the other hand, crocin (20 mg/kg) markedly improved learning and memory impairment and increased the levels of ERK, p.ERK, and CaMKII proteins in the hippocampus, compared to the BPA (100 mg/kg) group. Conclusion: Administration of crocin (20 mg/kg) improves memory and learning defects induced by BPA (100 mg/kg). The enhancement of the ‎p.ERK, ERK, and p. CaMKII protein levels in the hippocampus has an important role in crocin protective effects
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