Rong Chengbo, S. Shuang, Li Yang, Pang Xuejiao, Yu Liu, Shouxian Wang
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引用次数: 0
摘要
脂藻是一种具有优良营养和药用价值的蘑菇。然而，子实体产量低，这种真菌的商业种植潜力有限。本文将脂肪假单核菌株HS5和HS4进行单核杂交，获得279个杂交品系。以漆酶和菌丝生长速率为标记筛选杂交菌株，筛选出18株进行进一步分析。杂种菌株A10B4的产量最高，为165.91±12.56 g /袋，比菌株HS5和HS4分别高出31.34 g和74.48 g。A10B4的菌丝定殖时间为25.18±1.33 d，比HS5短5.64 d。通过简单序列重复分子标记对A10B4进行了鉴定。亲本和杂交菌株的PCR产物存在差异。因此，新开发的高产杂交种A10B4，命名为P. adiposa HS54，可能适合商业化栽培。
Breeding of a high-yield strain for commercial cultivation by crossing Pholiota adiposa
ABSTRACT Pholiota adiposa is a mushroom with excellent nutritional and medicinal properties. However, fruiting body yields are low, and the commercial cultivation potential of this fungus is limited. Here, 279 crossbred strains were obtained by the mono-mono crossing of monokaryotic strains derived from P. adiposa HS5 and HS4. Laccase enzymes and mycelial growth rates were used as markers to screen the crossbred strains, and 18 strains were selected for further analysis. Crossbred strain A10B4 displayed the highest yield (i.e. 165.91 ± 12.56 g per bag), which was 31.34 g and 74.48 g more than that of strains HS5 and HS4, respectively. The mycelial colonization time of A10B4 was 25.18 ± 1.33 d, which was 5.64 d shorter than that of HS5. A10B4 was characterized by inter-simple sequence repeat molecular markers. Differences in PCR products from parental and crossbred strains were observed. Therefore, the newly developed hybrid strain A10B4, named P. adiposa HS54 exhibiting high yield, might be suitable for commercial cultivation.
期刊介绍：
A Ciência e Agrotecnologia, editada a cada 2 meses pela Editora da Universidade Federal de Lavras (UFLA), publica artigos científicos de interesse agropecuário elaborados por membros da comunidade científica nacional e internacional.
A revista é distribuída em âmbito nacional e internacional para bibliotecas de Faculdades, Universidades e Instituições de Pesquisa.
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