利用F295遗传标记评价黄瓜对绿色斑驳花叶病毒抗性的有效性

D. D. Teplyakova
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摘要

的相关性。黄瓜绿色斑驳花叶病毒(CGMMV)是一种高度特化的病毒(托巴莫病毒群)。这是对葫芦的重大威胁。在高感染背景下,露天和保护地的黄瓜产量损失可接近100%。病毒的传播有多种方式。最危险的是被感染的种子材料。预防CGMMV的有效方法之一是创造对病毒疾病具有遗传抗性的杂交品种。为确定F295标记在抗性样品筛选中的有效性,进行了试验。将同一样品人工背景下的抗性测定结果与PCR分析结果进行比较,计算出遗传标记F295的效率为65%。由于有效性水平为平均水平,为了进行更客观的评估,有必要创建另一个-主要标记物,这将确定对CGMMV的直接抗性。F295标记的使用与其他抑制[1]采矿抗性的方法相结合是相关的,这将加快和提高育种过程的效率。
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The effectiveness of the use of the genetic marker F295 to assess the resistance of cucumber samples to the green mottled mosaic virus (CGMMV)
Relevance. Cucumber green mottled mosaic virus (CGMMV) is a highly specialized virus (group Tobamovirus). It is a significant threat to cucurbits. On a high infectious background, cucumber yield losses, both in open and protected ground, can be close to 100%. The spread of the virus occurs in many ways. The most dangerous is the infected seed material. One of the effective methods of preventing CGMMV is the creation of genetically resistant hybrids to viral diseases. An experiment was conducted to determine the effectiveness of the use of the marker F295 for the selection of resistant samples.Results. When comparing the results of determining resistance against an artificial background and the results of PCR analysis for the same samples, we calculated the efficiency of the genetic marker F295, which was 65%. Since the level of effectiveness is average, for a more objective assessment, it is necessary to create another – the main marker, which will determine the direct presence of resistance to CGMMV. The use of the F295 marker is relevant in combination with other methods for deter[1]mining resistance, which will speed up and increase the efficiency of the breeding process.
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