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Screening of vegetable pea breeding material on rust resistance at Omsk State Agrarian University 鄂木斯克国立农业大学筛选抗锈病的蔬菜豌豆育种材料
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-15-22
S. Kuzmina, N. Kazydub, L. Plotnikova, M. Y. Kukoleva
Relevance. Pea rust regularly damages the vegetable pea crop in the Omsk region. The harmfulness of the disease has begun to intensify in recent years; in years favorable for the development of the disease, the damage to susceptible varieties can be 100%. Therefore, breeders are forced to constantly search for new sources of resistance genes due to the rapidly developing rust pathogens. The goal of our research is to screen samples of a vegetable pea collection under field conditions in the southern forest-steppe of Western Siberia and identify sources of resistance.Material and Methodology. As an object for study, 72 samples of vegetable peas were taken from the Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC) and the “Federal Research Center of the All-Russian Institute of Plant Genetic Resources named after. N.I. Vavilov" (VIR). The research was conducted over four years from 2020 to 2023. in the conditions of the southern forest-steppe of the Omsk region. The records were carried out in accordance with the “Methodological instructions for studying the collection of grain legumes”.Results and Discussion. As a result of the studies, it was established that the resistance of vegetable peas to rust depends on both the genotype and weather conditions. Weather conditions had the greatest influence on the development of the disease (the factor’s share was 51.5%), the contribution of the genotype was also significant and amounted to 44.8%. The resistance of collection samples to rust in the conditions of the Omsk region varied from low (2.8 points) in warm and humid weather to very high (7.8 points) in dry and hot weather. The main influence on the spread of rust is exerted by the hydrothermal conditions of the “flowering – ripening” period (r=-0.98±0.11). Plant resistance to rust varies greatly depending on growing conditions (Cve=20.2-54.6%). The variety Darunok had the least variability of the trait over the years (Cve=20.2%), which will most consistently maintain resistance when the infectious load increases. As a result of screening of collection material of vegetable peas, sources of partial resistance to rust were identified for breeding in Western Siberia: varieties Darunok, Namorodok (Federal Scientific Vegetable Center (FSBSI FSVC)), Orel, Cruiser, Vityaz, Flagman-8, Nemchinovsky 46, B-579, In Memory of Khangildin (VIR collection).
相关性。豌豆锈病经常危害鄂木斯克地区的蔬菜豌豆作物。近年来,豌豆锈病的危害开始加剧;在有利于豌豆锈病发展的年份,易感品种的受害率可达 100%。因此,由于锈病病原体发展迅速,育种人员不得不不断寻找新的抗病基因来源。我们的研究目标是筛选西西伯利亚南部森林草原田间条件下的蔬菜豌豆样本,并确定抗性来源。作为研究对象,我们从联邦国家预算科学机构联邦科学蔬菜中心(FSBSI FSVC)和 "全俄植物遗传资源研究所联邦研究中心 "采集了 72 份蔬菜豌豆样本。N.I. Vavilov 命名的全俄植物遗传资源研究所联邦研究中心"(VIR)。这项研究从 2020 年到 2023 年在鄂木斯克州南部森林草原条件下进行,历时四年。记录是按照 "谷物豆科植物采集研究方法说明 "进行的。研究结果表明,蔬菜豌豆对锈病的抗性取决于基因型和天气条件。天气条件对病害发展的影响最大(占 51.5%),基因型的影响也很大,占 44.8%。在鄂木斯克地区的条件下,采集样本对锈病的抗性从温暖潮湿天气下的低抗性(2.8 分)到干燥炎热天气下的高抗性(7.8 分)不等。开花-成熟 "时期的水热条件对锈病传播的影响最大(r=-0.98±0.11)。植物对锈病的抗性因生长条件不同而有很大差异(Cve=20.2-54.6%)。品种 Darunok 的性状多年来变化最小(Cve=20.2%),当感染负荷增加时,该品种能最稳定地保持抗性。通过对蔬菜豌豆的收集材料进行筛选,确定了西西伯利亚育种用的部分抗锈病来源:品种 Darunok、Namorodok(联邦蔬菜科学中心(FSBSI FSVC))、Orel、Cruiser、Vityaz、Flagman-8、Nemchinovsky 46、B-579、In Memory of Khangildin(VIR 收集)。
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引用次数: 0
Effect of additional inoculation with Rhizobium leguminosarum on vegetable pea plants 额外接种豆荚根瘤菌对蔬菜豌豆植株的影响
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-85-91
O. V. Putina, O. V. Putin, V. A. Zhukov, A. Besedin
Relevance. Grain of vegetable pea varieties is processed at canning factories, frozen, dried, and consumed fresh. Industrial cultivation of peas can be made more environmentally friendly by using biological preparations that contain growth-stimulating bacteria, for example Rhizobium species. With additional inoculation with rhizobacteria, legumes have higher values of traits characterizing vegetative development, biochemical parameters and yield; their immunity and resistance to abiotic stress increases as well.Methodology. The aim of our study was to evaluate the effect of additional inoculation of Rhizobium leguminosarum strain 245a on biometric and physiological parameters and yield of vegetable pea varieties. The experiment was carried out at Krymsk Experiment Breeding Station – a branch of VIR (Krasnodar Region, Krymsk). We studied six commercial cultivar: Prima, Mayak, Alpha 2, Vesta, Parus (leafless morphotype) and Patriot. The seeds were treated the day before sowing with an aqueous solution of the biopreparation Rhizotorfin (produced by ECOS BIOPREPARATIONS, St.Petersburg) and with water in the control variant.Results. The use of the preparation had a growth-stimulating effect on vegetable pea plants. An increase in the length of plants, the height of attachment of the lower pod and the number of completed pods on the plant was found. An increase in the content of dry matter in the total biomass of plants, stems with leaves and pods was noted when using Rhizotorfin. Also, inoculation with rhizobacteria contributed to an increase in yield. The effectiveness of the biopreparation depended on the conditions of the year. The maximum difference between the values of the studied traits for the control and treatment variants was in 2023 - with a uniform distribution of precipitation and optimal temperatures during the growing season. A genotype-dependent reaction of vegetable pea plants to seed treatment with bacteria Rhizobium leguminosarum strain 245a was revealed. The most responsive to additional inoculation is the variety Parus of the leafless morphotype. The values of most biometric and physiological traits and yield in this genotype were significantly higher in the variant with the use of the Rhizotorfin biopreparation. Based on the results obtained, we recommend using the Rhizotorfin biopreparation when growing the variety Parus for commercial production.
相关性。蔬菜豌豆品种的谷物可在罐头厂加工、冷冻、烘干和新鲜食用。通过使用含有生长刺激细菌(如根瘤菌)的生物制剂,可使豌豆的工业化种植更加环保。在额外接种根瘤菌后,豆科植物的无性系发育、生化参数和产量的特征值更高;对非生物胁迫的免疫力和抵抗力也会增强。我们的研究旨在评估额外接种豆科根瘤菌 245a 菌株对蔬菜豌豆品种的生物计量、生理参数和产量的影响。实验在克雷姆斯克试验育种站进行,该站是 VIR 的一个分支机构(克拉斯诺达尔地区,克雷姆斯克)。我们研究了六个商业栽培品种:Prima、Mayak、Alpha 2、Vesta、Parus(无叶形态)和 Patriot。播种前一天用生物制剂 Rhizotorfin(由圣彼得堡 ECOS 生物制剂公司生产)的水溶液处理种子,对照组则用水处理种子。使用该制剂对蔬菜豌豆有促进生长的作用。植株的长度、下部荚果的附着高度和植株上完整荚果的数量都有所增加。使用根瘤菌素后,植株、茎叶和豆荚总生物量中的干物质含量都有所增加。此外,接种根瘤菌也有助于提高产量。生物制剂的效果取决于当年的条件。对照变体和处理变体的研究性状值之间的最大差异出现在 2023 年--生长季节降水分布均匀,温度适宜。研究发现,菜用豌豆植株对豆科根瘤菌 245a 株种子处理的反应取决于基因型。对额外接种反应最强烈的是无叶形态的 Parus 品种。在使用 Rhizotorfin 生物制剂的变种中,该基因型的大多数生物计量和生理特征值以及产量都显著提高。根据所获得的结果,我们建议在商业生产中种植 Parus 品种时使用 Rhizotorfin 生物制剂。
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引用次数: 0
Qualitative composition of phenolic compounds in leaves of Rhodiola rosea L. plants introduced in the conditions of the Moscow region 莫斯科地区引进的红景天植物叶片中酚类化合物的定性组成
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-54-60
T. Krol, V. Ossipov, D. Baleev
Relevance. Rhodiola rosea L. is a perennial herbaceous plant from the Crassulaceae family. The roots and rhizomes of R. rosea contain a range of biologically active compounds. This species is included in the State Pharmacopoeia of the Russian Federation. Medicinal preparations derived from them are widely used as adaptogens, general tonic and anti-stress agents. While the roots have been extensively studied, less attention has been given to the biologically active compounds found in the leaves of R. rosea. An introduced population of R. rosea grows on the territory of the botanical garden of medicinal plants of the All-Russian scientific research Institute of medicinal and aromatic plants (VILAR). The possibility of cultivating this species in the conditions of the Moscow region is being studied.Material and Methodology. The objective of this study was to investigate the composition of phenolic compounds in R. rosea leaves from VILAR biocollection using ultra-efficient liquid chromatography combined with mass spectrometry. Extraction was carried out with 80% acetone. An ultra-high performance liquid chromatographic system with a photodiode array detector and mass spectrometer was used for the analysis of phenolic compounds. Identification was carried out using UV- and mass spectrometry data.Results. As a result of UPLC-DD-MS analysis, peaks of 20 phenolic compounds were detected in R. rosea leaves, of which 19 compounds were tentatively identified. The leaf extract contained eight phenolic compounds from the group of galloyl-glucoses (three isomers of monogalloyl-glucose, trigalloyl-glucose, two isomers of tetragalloyl-glucose, two isomers of pentagalloyl-glucose), two gallotannins (isomers of hexagalloyl-glucose), five flavonoids (four herbacetin derivatives and one gossypetin), phenolic acids (caffeoyl-malic and ferulic acids), arbutin and esculetin. It has been shown that the biologically active substances of R. rosea provide prospects for the use of leaves as raw materials for the development of herbal preparations with anti-inflammatory, antiviral and antitumor effects.
相关性。红景天(Rhodiola rosea L.)是一种十字花科多年生草本植物。红景天的根和根茎含有一系列生物活性化合物。该物种已被列入《俄罗斯联邦国家药典》。从它们中提取的药剂被广泛用作适应剂、一般滋补剂和抗应激剂。虽然对蔷薇根进行了广泛研究,但对蔷薇叶中的生物活性化合物却关注较少。在全俄药用植物和芳香植物科学研究所(VILAR)的药用植物园内,生长着一个引进的玫瑰花种群。目前正在研究在莫斯科地区的条件下栽培该物种的可能性。本研究的目的是使用超高效液相色谱法结合质谱法研究 VILAR 生物采集的玫瑰叶中酚类化合物的组成。采用 80% 丙酮进行萃取。使用配有光电二极管阵列检测器和质谱仪的超高效液相色谱系统分析酚类化合物。利用紫外和质谱数据进行鉴定。通过 UPLC-DD-MS 分析,在玫瑰叶中检测到 20 种酚类化合物的峰值,其中 19 种化合物被初步鉴定。叶提取物中含有八种五倍子酰葡萄糖类酚类化合物(三种单甘氨酰葡萄糖异构体、三种三甘氨酰葡萄糖异构体、两种四甘氨酰葡萄糖异构体、两种五甘氨酰葡萄糖异构体)、两种没食子单宁(六没食子酰-葡萄糖的异构体)、五种黄酮类化合物(四种香草素衍生物和一种棉子素)、酚酸(咖啡酰苹果酸和阿魏酸)、熊果苷和鱼藤素。研究表明,玫瑰叶中的生物活性物质为利用玫瑰叶作为原料开发具有消炎、抗病毒和抗肿瘤作用的草药制剂提供了前景。
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引用次数: 0
Selection for immunity in FSBSI FSVC – history and modernity 在 FSBSI FSVC 中选择免疫--历史与现代
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-5-14
I. Engalycheva, Е. Kozar, A. Ushakov
Relevance. Maintaining the phytosanitary condition of vegetable and melon crops is an important component in the technologies of cultivation of new and promising varieties and hybrids, since losses from diseases of various etiologies reach 30% or more. This determines the need for immunological studies of the resistance of vegetable and melon crops to phytopathogens of various etiologies, where the basis of the strategy for creating new varieties and hybrids is the joint work of a breeder and a phytopathologist. To do this, it is important to conduct an annual study of the species composition in order to identify new harmful objects that have a negative impact on the profitability of production, as well as to develop measures to protect vegetable crops from the most harmful and economically significant pests and diseases.Methodology and Results. The extensive experimental network of the Federal Scientific Vegetable Center (FSBSI FSVC) in various ecological and geographical zones allows testing varieties and hybrids of vegetable and melon crops in various soil and climatic conditions, including evaluating them for the most harmful phytopathogens in these regions. This review article briefly highlights the main stages of the history of development and current trends in phytopathological research in Russia and our center. The most significant achievements of scientists of the FSBSI FSVC are presented, aimed at a comprehensive study of the current state of populations of pathogens on vegetable and melon crops, resistance factors controlling the interaction of organisms in pathosystems, including genetic ones, optimization and development of new methodological approaches in breeding for immunity and scientific justification of recommended modern protection systems.
相关性。保持蔬菜和瓜类作物的植物检疫条件是有前途的新品种和杂交种培育技术的重要组成部分,因为各种病原体造成的损失高达 30% 或更多。这就决定了有必要对蔬菜和瓜类作物对各种病原植物病原体的抗性进行免疫学研究,新品种和杂交种培育战略的基础是育种家和植物病理学家的共同工作。为此,必须每年对物种构成进行研究,以确定对生产利润产生负面影响的新的有害对象,并制定措施保护蔬菜作物免受危害最大、经济效益最高的病虫害的侵害。联邦蔬菜科学中心(FSBSI FSVC)在不同生态和地理区域拥有广泛的实验网络,可以在各种土壤和气候条件下对蔬菜和瓜类作物的品种和杂交种进行试验,包括对这些地区最有害的植物病原体进行评估。这篇综述文章简要介绍了俄罗斯和本中心植物病理学研究的主要发展历史阶段和当前趋势。文章介绍了俄罗斯科学院植物病理学研究中心(FSBSI FSVC)科学家取得的最重要成就,这些成就旨在全面研究蔬菜和瓜类作物上病原体种群的现状、控制病理系统中生物相互作用的抗性因素(包括遗传因素)、优化和开发免疫育种的新方法以及对推荐的现代保护系统进行科学论证。
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引用次数: 0
Methods of increasing herbage yield and seed productivity of Echinacea purpurea under conditions of Krasnodar krai 在克拉斯诺达尔边疆区条件下提高紫锥菊牧草产量和种子生产力的方法
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-105-110
R. R. Thaganov, N. Sidelnikov, O. L. Saibel
Relevance. For the production of pharmaceutical products from raw materials of Echinacea purpurea L., it is necessary to have a sufficient amount of high-quality seeds when planting production areas with high yields of aboveground mass.Methods. On Echinacea purpurea of the second year of vegetation in order to increase herbage yield and seed productivity, tests of organomineral fertilizer EcoFus (1.5 l/ha) with microfertilizers (Siliplant (0.5 l/ha) or Cytovit (1.0 l/ha). As a standard, we used double foliar feeding with Siliplant (0.5 l/ha). The above-ground mass was mowed in the third ten days of August. On seed plantations we tested: in the early growth phase EcoFus (1.5 l/ha), in the stemming phase – growth regulators Epin-extra (0.06 l/ha), Agat-25K (0.04 l/ha) and their tank mixtures. Seed collection was carried out in mid-September.Results. Double spraying with complex – EcoFus with microfertilizers, promoted activation of growth processes, and it resulted in 22-26% increase of above-ground mass yield, 5-7% increase of phenylpropanoids in it, their collection per hectare increased within 28-35%. A more effective complex was revealed: EcoFus (1.5 l/ha) with Siliplant (0.5 l/ha), which allowed to increase yield and collection of active substances by 26 and 35% in comparison with the control, and in comparison with the reference (Siliplant 0.5 l/ha) – by 11 and 15%, respectively. In order to increase seed productivity of Echinacea purpurea, spraying with EcoFus at the beginning of vegetation of plants and EcoFus complex with bioregulators Agat and Epin-extra in the phase of stemming was applied. Foliar fertilization with these tank mixtures resulted in earlier flowering of plants (by 2-4 days), increased the number of inflorescences by 9-10%, inflorescences with seeds by 14-15% and seed weight of one inflorescence by 12- 14%. Activation of generative processes of Echinacea purpurea had a positive effect on seed yield and their quality: yield increased by 21-25%, weight of 1000 pieces by 7-8% and their germination by 2-3%.
相关性。为了利用紫锥菊原料生产药品,在种植地上部分产量高的生产区时,必须有足够数量的优质种子。在紫锥菊第二年的植被上,为了提高草料产量和种子生产率,进行了有机矿物肥 EcoFus(1.5 升/公顷)与微肥(Siliplant(0.5 升/公顷)或 Cytovit(1.0 升/公顷))的试验。作为标准,我们使用 Siliplant(0.5 升/公顷)进行双重叶面喷施。在八月的第三个十天对地上部分进行除草。我们对种子种植园进行了测试:在生长初期使用 EcoFus(1.5 升/公顷),在茎秆生长期使用生长调节剂 Epin-extra(0.06 升/公顷)、Agat-25K(0.04 升/公顷)及其混合剂。种子采集于 9 月中旬进行。EcoFus 与微肥的双重喷洒促进了生长过程的激活,使地上部分的产量增加了 22-26%,其中的苯丙酮类化合物增加了 5-7%,每公顷的种子采集量增加了 28-35%。发现了一种更有效的复合物:与对照组相比,EcoFus(1.5 升/公顷)和 Siliplant(0.5 升/公顷)的产量和活性物质的采集量分别增加了 26% 和 35%;与参照组(Siliplant 0.5 升/公顷)相比,产量和活性物质的采集量分别增加了 11% 和 15%。为了提高紫锥菊的种子产量,在植株开始生长时喷洒 EcoFus,在茎叶生长阶段喷洒 EcoFus 复配生物调节剂 Agat 和 Epin-extra。使用这些混合物进行叶面施肥可使植物提前开花(2-4 天),花序数量增加 9-10%,带有种子的花序数量增加 14-15%,单个花序的种子重量增加 12-14%。激活紫锥菊的生成过程对种子产量和质量有积极影响:产量增加 21-25%,1000 粒种子重量增加 7-8%,发芽率增加 2-3%。
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引用次数: 0
Effect of inoculation with microbiological preparations on morphological characteristics and yield of vegetable soybean 接种微生物制剂对菜豆形态特征和产量的影响
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-99-104
F. E. Mullo Panoluisa, E. V. Romanova, C. A. Salazar Flores
Relevance. Vegetable soybean is a special variety with high added value, consumed fresh and harvested at the stage technical ripeness (R6). Accession-A is one of the promising vegetable soybean materials, studied and identified as a result of work that was carried out jointly with the Federal Scientific Vegetable Center in the Moscow Region (FSVC) and the Agrobiotechnology Department of the Agricultural Technological Institute (ATI) of the RUDN University named after Patrice Lumumba. The use of microorganisms to promote atmospheric nitrogen fixation is a common practice in soybean production.Methodology. The purpose of this study was to evaluate the effect of two microbiological preparations based on Sinorhizobium fredii and Bradyrhizobium jaроniсum in order to identify which microbiological preparation gives the best results in terms of morphological indicators and vegetable soybean yield under the conditions region of the Non-Chernozem zone of the Russian Federation. The object of the study was accession-A of vegetable soybean. Two types of treatment were carried out: B1 – treatment with a preparation with Bradyrhizobium jaronicum (1 l/t of seeds), B2 – treatment with a preparation with Sinorhizobium fredii (1 l/t of seeds). Control without treatment (K).Results. According to statistical analysis of biometric indicators, it was found that treatment with S. fredii (B2) had the highest average values for indicators of stem diameter (11.50 mm), lower pod attachment height (11.19 cm), number of branches (3.40 pcs.) and the number of productive nodes (27.48 pcs.). Preparation B2 improved the structure of the crop, influencing the obtainment of the highest indicators of plant weight (30.47 g), seed weight per plant (15.33 g), weight of 1000 seeds (171.25 g), number of seeds per plant (98.50 pcs.), number of seeds in a bean (2.89 pcs.) and seed yield (3.11 t/ha). The use of microbiological preparations based on B. japonicum and S. fredii gave better results compared to the control treatment.
相关性。蔬菜大豆是一种具有高附加值的特殊品种,新鲜食用,在技术成熟期(R6)收获。通过与莫斯科地区联邦蔬菜科学中心(FSVC)和以 Patrice Lumumba 命名的 RUDN 大学农业技术研究所(ATI)农业生物技术部联合开展工作,研究并确定了 Accession-A 是有前途的蔬菜大豆材料之一。使用微生物促进大气固氮是大豆生产中的常见做法。本研究的目的是评估基于 Sinorhizobium fredii 和 Bradyrhizobium jaроniсum 的两种微生物制剂的效果,以确定哪种微生物制剂在俄罗斯联邦非切尔诺泽姆地区条件下的形态指标和蔬菜大豆产量方面效果最佳。研究对象是蔬菜大豆的加入物-A。进行了两种处理B1 - 使用 Bradyrhizobium jaronicum 制剂(1 升/吨种子)处理,B2 - 使用 Sinorhizobium fredii 制剂(1 升/吨种子)处理。结果。根据生物计量指标的统计分析发现,使用 S. fredii(B2)处理的茎干直径(11.50 毫米)、下部豆荚附着高度(11.19 厘米)、分枝数(3.40 个)和生产节数(27.48 个)的平均值最高。制剂 B2 改善了作物的结构,影响了植株重量(30.47 克)、每株种子重量(15.33 克)、1000 粒种子重量(171.25 克)、每株种子数量(98.50 粒)、每粒种子数量(2.89 粒)和种子产量(3.11 吨/公顷)等最高指标的获得。与对照处理相比,使用基于日本酵母菌(B. japonicum)和酵母菌(S. fredii)的微生物制剂取得了更好的效果。
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引用次数: 0
The Role of Agroforestry in Ecosystem Services and Mitigation of Climate Change 农林业在生态系统服务和减缓气候变化方面的作用
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-111-119
A. Worku
Relevance. Agroforestry systems are believed to provide a multitude of ecological services. It is thought that agroforestry enhances resilience to the impacts of climate change and aids in adaptation by supporting diverse land use practices, sustainable lifestyles, and income streams, as well as increasing productivity in both forests and agriculture, and reducing weather-related losses in production.Results and Discussion. The aim of this review was to present genuine evidence on the role of agroforestry in ecosystem conservation and mitigation of climate change impacts. Compared to monocropping and open cereal-based agriculture, agroforestry has made a more significant contribution to ecosystem conservation and in reducing carbon dioxide emissions. However, it has been found that agroforestry contributes less to carbon sequestration than natural forests. Carbon sequestration through above-ground and underground biomass, carbon emission reduction from deforestation, and microclimate adjustment are key measures for mitigating climate change. Agroforestry systems provide essential ecosystem services, such as food, fuel wood, fodder, income, and improved soil production, which enable communities to cope better with the impacts of climate change. Therefore, agroforestry must be given significant attention if it is to play a crucial role in ecosystem management.
相关性。农林系统被认为可提供多种生态服务。人们认为,农林业通过支持多样化的土地利用方式、可持续的生活方式和收入来源,以及提高森林和农业的生产力和减少与天气有关的生产损失,可增强对气候变化影响的抵御能力并帮助适应气候变化。本综述旨在提供有关农林业在生态系统保护和减轻气候变化影响方面作用的真实证据。与单一作物种植和开放式谷物农业相比,农林业对生态系统保护和减少二氧化碳排放的贡献更为显著。然而,研究发现,农林业的固碳作用低于天然林。通过地上和地下生物量固碳、减少毁林造成的碳排放以及调整小气候是减缓气候变化的关键措施。农林业系统提供了重要的生态系统服务,如食物、薪材、饲料、收入和改良土壤,使社区能够更好地应对气候变化的影响。因此,农林业要想在生态系统管理中发挥关键作用,就必须得到高度重视。
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引用次数: 0
Diagnostics of mineral nutrition of carrot and red beet at organic and mineral fertilization systems 有机肥和矿物质肥系统中胡萝卜和红甜菜矿物质营养诊断
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-69-79
S. V. Belova
Relevance. In the transition to highly productive and environmentally friendly, in particular, organic agriculture and production of safe and high-quality products, which is a priority direction of strategies of scientific and technological development of the Russian Federation, one of the necessary links is the creation and control of optimal conditions of plant nutrition, i.e. provision of plants with nutrients in the right combination throughout the vegetation. Achievement of such conditions is possible through soil and plant nutrition diagnostics, which in turn allows to establish the ability of crops to assimilate nutrients from the soil and adjust the doses of mineral fertilisers as top dressings.Material and Methods. On alluvial meadow soil in the conditions of the non-Black Earth region of Russia, the effectiveness of root feeding with macronutrients (NPK) was studied according to the results of plant and soil diagnostics of table root crops nutrition when grown on organic and mineral fertiliser systems. Plant and soil diagnostics of mineral nutrition was carried out according to the methods of Zerling V.V. (1990) and Magnitsky K.P. (1972). Two crops were tested during the experiment: red beet variety Mulatka and garden carrot variety Losinoostrovskaya 13.Results and Discussion. Root fertilizers on the basis of nutrition diagnostics on the basis of soil and leaf petiole analysis made significant changes in the dynamics of basic nutrients in the soil, there was an increase in nitrate nitrogen and mobile potassium in comparison with the control variant, with natural soil supply of mobile phosphorus (more than 250 mg/kg), which had a positive effect on the root nutrition of plants. At the main application of mineral and organic (biocompost BIUD) fertilizers, root fertilizers according to nutrition diagnostics the carrot yield 51,2-63,9 t/ha with standard 87,4- 94,6%, red beet 41,7-54,2 t/ha with standard 80,7-92,3% were obtained. Root feeding of carrots by soil diagnostics N52K14 on the background of biocompost gave 24.9% increase to the control, by plant diagnostics N17 gave 16.6%; red beet by soil diagnostics N65K45 – 30% increase, by plant diagnostics N54K30 – 23.7%
相关性。俄罗斯联邦科技发展战略的优先方向是向高产、环保、特别是有机农业和安全优质产品的生产过渡,其中一个必要的环节是创造和控制植物营养的最佳条件,即在整个植被过程中为植物提供适当组合的养分。通过土壤和植物营养诊断,可以确定作物从土壤中吸收养分的能力,并调整矿物肥料的剂量。在俄罗斯非黑土区的冲积草甸土壤上,根据对有机肥和矿质肥料体系下种植的块根作物营养的植物和土壤诊断结果,研究了用大量元素(氮磷钾)喂养块根的有效性。植物和土壤矿质营养诊断是根据 Zerling V.V. (1990) 和 Magnitsky K.P. (1972) 的方法进行的。试验期间测试了两种作物:红甜菜品种 Mulatka 和园艺胡萝卜品种 Losinoostrovskaya 13。根据土壤和叶柄分析进行营养诊断的根肥使土壤中基本养分的动态发生了显著变化,与对照变体相比,硝态氮和移动钾增加了,土壤中移动磷的自然供应量(超过 250 毫克/千克)增加了,这对植物根部营养产生了积极影响。在主要施用矿物肥料和有机肥料(生物堆肥 BIUD)的情况下,根据营养诊断施用根肥,胡萝卜产量为 51.2-63.9 吨/公顷,标准为 87.4-94.6% ;红甜菜产量为 41.7-54.2 吨/公顷,标准为 80.7-92.3% 。在施用生物堆肥的背景下,用土壤诊断剂 N52K14 给胡萝卜根部施肥比对照组增产 24.9%,用植物诊断剂 N17 给胡萝卜根部施肥增产 16.6%;用土壤诊断剂 N65K45 给红甜菜施肥增产 30%,用植物诊断剂 N54K30 给红甜菜施肥增产 23.7
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引用次数: 0
Comparative evaluation of Raphanus sativus var. lobo defense efficiency against Contarinia nasturtii using foliar application of sodium selenate, ionic silicon form and garlic extract 使用硒酸钠、离子硅形式和大蒜提取物叶面喷施,比较评估 Raphanus sativus var.
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-23-27
N. А. Golubkina, V. Zayachkovsky
Relevance. Contarinia nasturtii Keiffer is one of the most dangerous insect pests of Brassicaceae plants dramatically affecting plant yield and seed productivity.Material and Methods. The effect of single foliar application of sodium selenate, ionic form of silicon (Siliplant fertilizer) and garlic extract on the efficiency of Raphanus sativus lobo defense against Contarinia nasturtii infestation was evaluated in condition of greenhouse. Concentrations of the applied reagents were: sodium selenate 26.4 mM; Siliplant – 1 ml/l; garlic extract – 8 g/l. Seed productivity and C. nasturtii infestation levels under Se, Si and garlic extract administration under infestation were determined along with the analysis of plant antioxidant status and sugar content.Results. Foliar application of Siliplant singular or in combination with Se provided the highest seed productivity and mass of 1000 seeds. Plants treated with Si, garlic extract and Si+Se demonstrated lack of C. nasturtii infestation only a week after the preparation supply. Contrary, selenate provided less pronounced defense. Among biochemical parameters tested the values of the total dissolved solids were the highest in plants treated with Se and Se+Si. Changes in the intensity of plant defense due to garlic, Se and Si supply were reflected in the increase of the total phenolic content compared to the values typical for control plants and in a significant accumulation increase of disaccharides, participating in tissue recovery after herbivory attack. Silicon application also provided an increase of selenium accumulation by lobo leaves.
相关性。菜青虫(Contarinia nasturtii Keiffer)是十字花科植物最危险的害虫之一,严重影响植物产量和种子生产力。在温室条件下,评估了硒酸钠、离子型硅(Siliplant 肥料)和大蒜提取物的单次叶面喷施对Raphanus sativus lobo 抵御Contarinia nasturtii 侵染效率的影响。施用试剂的浓度为:硒酸钠 26.4 mM;硅肥 - 1 ml/l;大蒜提取物 - 8 g/l。在施用 Se、Si 和大蒜提取物的情况下,测定了种子产量和 C. nasturtii 的虫害水平,并分析了植物的抗氧化状态和含糖量。单一叶面喷施或结合喷施 Siliplant 与 Se 可提供最高的种子产量和 1000 粒种子的质量。使用 Si、大蒜提取物和 Si+Se 处理过的植物在施药一周后才显示出没有棉铃虫侵扰。相反,硒酸盐的防御效果不明显。在测试的生化参数中,用 Se 和 Se+Si 处理的植物的总溶解固体值最高。与对照植物的典型值相比,大蒜、硒和硅对植物防御强度的影响体现在总酚含量的增加和二糖积累的显著增加上,二糖参与了草食动物攻击后的组织恢复。施硅还能增加龙须菜叶片的硒积累。
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引用次数: 0
Assessment of the resistance of inbred table beet lines to cercosporosis (Cercospora beticola Sacc.) in field and laboratory conditions 在田间和实验室条件下评估近交甜菜品系对盘孢(Cercospora beticola Sacc.)的抗性
Pub Date : 2024-07-09 DOI: 10.18619/2072-9146-2024-4-28-34
L. N. Timakova, K. L. Alekseeva, L. M. Sokolova
Relevance. Cercospora leaf spot (Сercospora beticola) is a widespread disease of table beet that reduces the yield of root crops in many beet-growing regions. Annual losses from cercosporosis amount to 30-40%, and in the years of epiphytotic diseases – 50% or more. The fungus has a complex intraspecific structure, includes many pathotypes and races, which should be taken into account when breeding for resistance. The main method of creating resistant forms of table beet to C. beticola is the selection of cercospore-resistant lines in the natural manifestation of the disease and on artificial backgrounds. In open ground conditions, it is not always possible to assess the resistance of table beet to cercosporosis, since cercosporosis is weak in some hot and dry years. Therefore, laboratory assessment methods are important to accelerate the breeding process. The purpose of the research is to carry out an immunological analysis of beet lines in the field and laboratory conditions for the selection of sustainable material.Materials and methods. The assessment of beet variety samples for resistance to cercospora blight was carried out in open ground conditions on the basis of the All-Russian Scientific Research Institute of Vegetable Growing – branch of the FSBSI Federal Scientific Vegetable Center (Moscow region). In laboratory conditions, artificial infection of separated red beet leaves was carried out. The objects of the study were inbred lines of red beets of the 1st-6th generation.Results and discussion. As a result of evaluation and selection, red beet lines No. 713 and 790 with increased resistance to cercospora blight were identified. It has been established that for practical breeding it is necessary to constantly and strictly select for resistance to cercospora blight. In laboratory conditions, a method of artificial infection of separated leaves has been developed, which allows for a short period of time to carry out a preliminary assessment of a large number of beetroot samples for resistance to cercospora blight.
相关性。甜菜叶斑病(Сercospora beticola)是甜菜的一种普遍病害,在许多甜菜种植区会降低根茎作物的产量。每年,甜菜叶斑病造成的损失达 30-40%,在发生附生病害的年份,损失达 50%,甚至更多。真菌具有复杂的种内结构,包括许多病型和种族,在培育抗性时应考虑到这一点。培育甜菜抗甜菜夜蛾的主要方法是在自然条件下和人工背景下选育抗球孢子的品系。在露地条件下,并不总能评估甜菜对盘孢菌病的抗性,因为盘孢菌病在一些炎热干燥的年份很弱。因此,实验室评估方法对加快育种进程非常重要。这项研究的目的是在田间和实验室条件下对甜菜品系进行免疫学分析,以选择可持续材料。在全俄蔬菜种植科学研究院(FSBSI 联邦蔬菜科学中心分部)(莫斯科地区)的基础上,在露地条件下对甜菜品种样本进行了抗盘孢菌枯萎病能力评估。在实验室条件下,对分离的红甜菜叶片进行了人工感染。研究对象为第 1-6 代红甜菜近交系。经过评估和选育,确定了 713 号和 790 号红甜菜品系,这两个品系具有更强的抗盘孢菌枯萎病能力。实践证明,在实际育种过程中,有必要不断严格筛选出具有抗盘孢菌枯萎病能力的品系。在实验室条件下,已开发出一种对分离叶片进行人工感染的方法,可在短时间内对大量甜菜样品进行抗盘孢菌枯萎病的初步评估。
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引用次数: 0
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Vegetable crops of Russia
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