电化学生物传感器在农药检测中的应用2010-02-01 2010-06-30 2010-07-21

G. Mostafa
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引用次数: 51

摘要

基于酶、抗体、细胞和dna的生物传感器已被开发用于农药检测。酶法测定农药最常用的方法是抑制选定酶的活性,如胆碱酯酶、酸性磷酸酶、抗坏血酸氧化酶、乙酰乳酸合成酶和醛脱氢酶。利用各种电化学信号传感器和不同的电极,开发了酶促生物传感器。还讨论了用于形成生物识别界面的各种固定方案:此外,对免疫传感器的再生、单次扩增和小型化技术进行了评估。批和流动注射分析与酶生物传感器是最密集的发展。它包括,在未来,紧凑,一次性和便携式设备,专门设计用于现场分析具有高灵敏度,选择性;阵列和多传感器的发展将继续生物传感器的另一个深入研究领域。
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Electrochemical Biosensors for the Detection of Pesticides~!2010-02-01~!2010-06-30~!2010-07-21~!
Biosensors have been developed for the detection of pesticides using integrated enzymes, antibodies, cell and DNA-based biosensors. Enzymatic determination of pesticides is most often based on inhibition of the activity of selected enzymes such as cholinesterase, acid phosphatase, ascorbate oxidase, acetolactate synthase and aldehyde dehydrogenase. Enzymatic Biosensors were developed using various electrochemical signal transducers and different electrodes. Various immobilization protocols used for the formation of a biorecognition interface are also discussed: In addition, techniques of regeneration, single amplification, and miniaturization are evaluated for the development of immunosensor. Both batch and flow-injection analyses with enzyme biosensors are most intensively developed. It included that, in the future, com- pact, disposable and portable devices especially designed for in-field analysis with high sensitivity, selectivity; develop- ment of arrays and multiple sensors will continue another area of intensive research for biosensors.
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