蛙底胃和小肠糖结合物组织化学的研究

S. Sancar-Bas, Engin Kaptan, M. Inceli, A. Sezen, Huseyin Us
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引用次数: 5

摘要

采用凝集素作为特异性探针结合末端糖残基和光镜下标准组织化学等技术,对豚鼠胃底部和小肠的粘蛋白进行了研究。在这项研究中,我们关注消化道不同区域的形态功能多样性及其可能的生理和进化意义。我们使用了以下标准组织化学技术:含周期酸-希夫(PAS),阿利新蓝(AB) pH 1.0和2.5,甲苯胺蓝,醛fucsin和溴酚蓝。凝集素组织化学采用DBA、WGA、PNA、ConA和UEA-I五种不同的凝集素。胃底部产生的糖缀合物主要由中性粘蛋白和强硫酸粘蛋白组成,含有α- n -乙酰- d -半乳糖胺和n -乙酰-β- d -氨基葡萄糖胺残基。此外,从小肠分泌的糖缀合物主要由硫酸盐和含有n -乙酰基-β- d -氨基葡萄糖的中性粘蛋白组成。由此可以推断,利迪布达蛙两个消化道区糖缀合物类型和糖残基的差异可能与粘蛋白的特殊功能和流变特性有关。
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Glycoconjugate Histochemistry in the Fundic Stomach and Small Intestine of the Frog (Rana ridibunda)
Mucins in the fundic stomach and small intestine of Rana ridibunda were studied by using the techniques such as lectins as a specific probe binding terminal sugar residues and standard histochemistry in light microscopic level. In this study, we focused on morphofunctional diversities of different regions of the digestive tract and their feasible physiological and evolutionary implications. We used the following standard histochemical techniques: contained periodic acid-Schiff (PAS), Alcian blue (AB) pH 1.0 and 2.5, toluidin blue, aldehyde fucsin and bromfenol blue. For lectin histochemistry, five different lectins were used namely, DBA, WGA, PNA, ConA and UEA-I. The glycoconjugate produced in the fundic part of the stomach is composed of mainly neutral mucins and strongly sulphated acid mucins with α-N-acetyl-D-galactosamine and N-acetyl-β-D-glucosamine residues. Besides, the glycoconjugate secreted from the small intestine consists of mostly sulphated sialo and neutral mucins with N-acetyl-β-D-glucosamine moieties. It can be concluded that the differences in glycoconjugates types and the sugar residues in two digestive tract regions of Rana ridibunda may be related to special functions and rheological characteristics of the mucins.
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