向日葵枯萎病生物接种综合治理研究

Lakshman Ss, Rao Ch, Er, Godke Mk
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引用次数: 1

摘要

向日葵是一种一年四季都能生长的新型油料作物。在西孟加拉邦,这种作物主要生长在rabi季节,该季节遭受许多生物胁迫,其中土壤传播的疾病具有重要的经济意义。AICRP对Nimpith中心的向日葵进行了实地研究,以确定克服向日葵枯萎造成的产量损失的最佳管理方法。结果表明,大多数真菌和细菌拮抗剂在体内条件下对土壤传播疾病有效。真菌拮抗剂木霉(Trichoderma viride, Tv)和哈茨木霉(Trichoderma harzianum, Th)分离物和细菌拮抗剂假单胞菌(Pseudomonas fluorescens, Pf)分离物作为生物制剂使用时,对该种子传播疾病有很好的防治效果。在体内条件下,采用种子、土壤和种子+土壤处理,观察上述拮抗剂(生物制剂)组合对向日葵种子萌发、出苗、幼苗早期生长和抗枯萎病的效果。DRSH-1。后期研究进一步表明,与仅施用种子或土壤的拮抗剂相比,施用种子+幼苗+土壤的拮抗剂可以降低农民田间的枯萎率,从而提高种子产量。在向日葵幼苗期和成熟期,两种生物接种剂组合施用时,向日葵植株的死亡率最低。荧光剂(5.0g/点燃5.0g +5.0g/ kg种子)和组合作为种子接种剂,并在第一次和第二次灌溉前在冠区喷洒。示范田研究的历年数据表明,采用改良向日葵种植方法并施用生物接种剂的农民在效益:成本(B:C)比方面的经济优势更高,其效益:成本(B:C)比为1.41(2014-15)和1.45(2015-16),远高于常规种植系统/农民实践,1.06(2014-15)-1.18(2015-16)。本研究的结论是,翠绿T.、荧光P.和哈兹T.及其组合对西孟加拉邦向日葵枯萎病的有效治理具有重要作用。
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An Approach to Integrated Management of Sunflower Wilt through Bio-Inoculants
Sunflower is a new emerging oilseed crop which can grown through-out the year. In West Bengal this crop mainly grow in rabi season which suffers many biotic stresses, out of which soil borne diseases are of great economic importance. A field study was conducted by AICRP on Sunflower, Nimpith Centre to identify the best management approaches to overcome the yield loss due to wilt of sunflower. It was observed that most of the fungal and bacterial antagonists tested were found effective against soil borne diseases in-vivo conditions. The isolates of fungal antagonists Trichoderma viride (Tv), and Trichoderma harzianum (Th) and one isolate of bacterial antagonist Pseudomonas fluorescens (Pf) were found very effective to control this seed borne disease when used as bio-agents. The above antagonists (bio-agents) with their combinations found highly effective against all the soil borne pathogens causing the sunflower wilt under in vivo conditions were used for seed, soil and seed+soil treatments to observe their effectiveness on seed germination, seedling emergence, early seedling growth and resistance of wilt incidence in sunflower cv. DRSH-1. Post studies further revealed that seed+seedling+soil application of the antagonists resulted in lesser wilt incidence compared to only seed or soil application of these antagonists resulting higher seed yield in farmer’s field. The lowest mortality of sunflower plants either in seedling stage or in mature stage also observed when the bio-inoculants were used in combination (T. harzianum+P. fluorescens (5.0g/lit 5.0g +5.0g/ kg of seed) and when the combination was used as seed inoculants and also used for spraying at the crown region before first and second irrigation. The data across of the years of study on demonstration fields, indicated that economic advantage in terms of the Benefit: Cost (B:C) ratio of the farmers under improved method of sunflower cultivation with seed treatments and application of bioinoculants results higher Benefit: Cost (B:C) ratio higher was recorded 1.41 (2014-15) and 1.45 (2015-16) which were much higher compared to conventional cultivation systems/ Farmer’s Practice, 1.06 (2014-15)-1.18 (2015-16). The conclusion of the present study is that T. viride, P. fluorescens and T. harzianum and their combinations have significant role on to effective management of sunflower wilt in West Bengal.
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