重要药用植物毛竹在体内和离体繁殖的比较生化评价

IF 0.8 4区 生物学 Q3 AGRONOMY Legume Research Pub Date : 2018-05-20 DOI:10.18805/LR-4016
A. Malik, K. Sehrawat, Anil.k. Ahlawat, A. R. Sehrawat
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引用次数: 2

摘要

药用植物在生物技术领域引起了极大的兴趣,因为大多数制药工业在一定程度上依赖于植物来生产药用化合物。通过调节培养条件,可以提高植物中各成分的生物活性。本研究采用MS培养基,加入BAP、Kinetin、NAA等佐剂,培养毛毛Alhagi的叶片和节段。单独添加BAP(1.0和2.0 mg/l)和NAA (0.1 mg/l)的MS培养基有利于愈伤组织的发生和芽的形成。BAP(2.0和5.0mg/l) + NAA(0.1mg/l) +动蛋白(0.25mg/l) +抗坏血酸(50mg/l)和硫酸腺嘌呤(25mg/l)、柠檬酸(25mg/l)对芽增殖和芽增殖效果最好。在添加IAA和IBA (0.5mg/ leach)的MS培养基上生根效果最好。对不同生长阶段的培养材料进行了初级代谢物的定量生化评价。田间栽培植株的叶片和再生植株的芽中碳水化合物、总蛋白质和氨基酸含量最高。进一步得出毛藻是一种丰富的初级代谢物来源,可作为工业原料。
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A comparative biochemical evaluation of in vivo and in vitro propagated Alhagi maurorum: an important medicinal plant
Medicinal plants are of great interest in the field of biotechnology as most of the drug industries depend in part on plants for production of pharmaceutical compounds. Biological activities of the phyoconstituents in plants could be enhanced by manipulation of the culture conditions. In the present investigation leaves and nodal segments of Alhagi maurorum were cultured on MS medium with BAP, Kinetin, NAA and other adjuvants. MS medium with BAP (1.0 and 2.0 mg/l) alone and in combination with NAA (0.1 mg/l was good for initiation of calli and bud formation. BAP (2.0 and 5.0mg/l) with NAA(0.1mg/l) plus kinetin 0.25mg/l with ascorbic acid 50mg/l and adenine sulphate, citric acid and arginine 25mg/l each was found to be the best for bud proliferation and shoot multiplication. Best rooting was found on MS medium supplemented with IAA and IBA (0.5mg/ leach). Cultured materials at different growth phase were evaluated for their biochemical estimation of primary metabolites quantitatively. Maximum content of carbohydrates, total proteins and amino acids were noticed in leaves of field grown plants and shoots of regenerated plantlets. It is further concluded that Alhagi maurorum serve as a rich source of primary metabolites which can be used as raw materials in industry.
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来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
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