ECIS to assess human skin cell photo-oxidative damage

Various bioactive molecules help to cure skin diseases from solar radiation. Exploring the cellular behavior and real-time estimation of photo-oxidative stress in skin cells require advanced sensitive methods. In our present research describes an analytical tool, which unprecedented, simple and real-time in vitro light setup was established using a bioimpedance system to measure the skin cell photo-oxidative damage and protective effect of drugs. In order to overcome the stability of drugs, biodegradable permeation enhanced poly(D,L-lactide-coglycolide) (PLGA) nanoparticles (NPs) had been used to improve the therapeutic efficacy. Quercetin-loaded PLGA NPs were synthesized. Structural characterization confirmed a spherical mono-dispersed particle size distribution of 96 nm. In the present study, investigated photo-oxidative stress damage and addressed the HDFn cell behavior using continuous measurement of electrical cell-substrate impedance sensing technique, and compared with standard viability assay. Also photo-protective effects of prepared quercetin-loaded NPs and raw drugs were examined by real-time manner. The produced NPs shows high encapsulation efficiency, less toxicity, enhanced cellular uptake. Also the NPs shows high photo-protective effect on HDFn cells through scavenging of free radicals, and it can shows their better action to target sites. The proposed system identifies the phototoxic effects in skin cells and provides high throughput drug screening during early stages of photo-oxidative stress, and would be of broad interest in the field of therapeutics.