极高频电磁辐照处理对小麦种子萌发、生长及淀粉酶活性的影响

G. Poghosyan, M. Mikaelyan, P. Vardevanyan
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摘要

本文研究了超低频(42.2 GHz和50.3 GHz)低强度(非热,通量0.06 mW/cm2)电磁辐射(EMI)播前处理对小麦种子萌发、早期生长和α-淀粉酶活性的影响。在种子萌发过程中测定萌发能和萌发力、萌发指数、茎长和根长以及鲜重。实验室试验采用完全随机设计,设4个处理(对照、5 min - T5、10 min - T10和15 min - T15), 3个重复。结果表明,两种频率的EHF EMI播前处理均有利于小麦种子的萌发和生长,且在水的共振频率50.3 GHz处表现得更为明显。结果表明,50.3 GHz干扰10 min显著提高了种子萌发能(6.5%)和发芽力(7.6%)。苗期茎高(11%)、根长(12%)和鲜重(15%)均较对照显著提高。此外,α-淀粉酶活性的测定表明,emi处理使α-淀粉酶活性提高了2倍以上,从而可能促进了小麦的萌发。
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EFFECT OF EXTREMELY HIGH FREQUENCY ELECTROMAGNETIC IRRADIATION TREATMENT ON GERMINATION, GROWTH AND AMYLASE ACTIVITY OF WHEAT SEEDS
In this work, the effect of low-intensity (non-thermal, flux capacity 0.06 mW/cm2) electromagnetic irradiation (EMI) of extremely high frequency (EHF) (42.2 GHz and 50.3 GHz) as pre-sowing treatment on germination, early growth and α-amylase activity of wheat seeds (Triticum aestivum L.) has been studied. The measurements conducted during seed germination were the germination energy and germination power, germination index, shoot and root length, as well as fresh weight. The labo­ratory experiment followed a completely randomized design, with four treatments (control, 5 min – T5, 10 min – T10, and 15 min – T15) and three replications. It was shown that EHF EMI pre-sowing treatment at both used frequencies favored the germination and growth of wheat seeds, and results were more expressed at a frequency of 50.3 GHz – the resonant frequency of water. We found that EMI with 50.3 GHz for 10 min significantly improved germination energy (by 6.5%) and germination power (by 7.6%). At the same time, shoot high (by 11%), root length (by 12%) and fresh weight (15%) were enhanced significantly at seedling stage compared with the control. Furthermore, the measured activity of α-amylase reviled that EMI-treatment increased the enzyme activity more than 2-fold, thereby probably promoting wheat germination.
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