Alwi Millang, Widiawati, Umrah, Orryani Lambui
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摘要

丹戎阿皮自然保护区位于苏拉威西省中部东条乌纳乌纳县的托米尼湾。本研究旨在获得作为抗微生物生产者的本地细菌分离株。本研究采用的是琼脂双层法(琼脂双层法)的目的取样和筛选方法。使用三种微生物试验病原菌(金黄色葡萄球菌、大肠杆菌和白色念珠菌)进行孔扩散(孔)法检测抗菌效力。在每个孔中分别给予500 μL的分离菌,在37℃的温度下孵育24小时,持续48小时。观察到的参数是在井周围形成一个清晰的区域。抗菌细菌筛选结果。获得了多达24株的细菌分离株。通过观察所得抑菌带进行抑菌力测试,得到5株菌株S2U1、S3U3、S7U3、S9U3和S10U3在12.25 mm的温和带中抑菌力最强,但仅对金黄色葡萄球菌有效,而S9U3对金黄色葡萄球菌5.50 mm、大肠杆菌9.25 mm、白色念珠菌5.50 mm的抑菌力最强。关键词:细菌探索,抗菌剂,自然保护区
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EKSPLORASI BAKTERI LOKAL PENGHASIL ANTIMIKROBA DI CAGAR ALAM TANJUNG API TELUK TOMINI SULAWESI TENGAH, INDONESIA
ABSTRACT Tanjung Api Nature Reserve is a headland located in Tomini Bay Ampana District Tojo Una-Una Regency, Central Sulawesi Province. This study aims to obtain local bacteria isolates as antimicrobial producers. The method used in this research is purposive sampling and screening method using "Agar Dua Lapis" (Double Layer Method). Testing antimicrobial potency using the method to make Well Diffusion (well) using three pathogens of microbial tests (S. aureus, E. coli, and C. albicans). In each well hole is given 500 μL of bacterial isolates each and incubated at a temperature of 37 oC for 24 hours and continued for 48 hours. The observed parameter is a clear zone formed around the well. Antimicrobial-producing bacteria screening results. obtained as many as 24 isolates of bacteria. Testing of antimicrobial potency by looking at the resulting inhibitory zone is obtained five isolates namely S2U1, S3U3, S7U3, S9U3, and S10U3 are the highest isolates of the bland zone of 12.25 mm but only against S. aureus, while S9U3 is the best isolate because it is able to inhibit the four test microbes namely S. aureus by 5.50 mm, E. coli 9.25 mm, and C. albicans 5.50 mm.   Keywords:  Exploration of bacteria, antimicrobials, and Nature Reserve
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