DNA标记在鹰嘴豆抗枯萎病及遗传多样性测定中的应用

IF 0.8 4区生物学 Q3 AGRONOMY Legume Research Pub Date : 2018-05-20 DOI:10.18805/LR-407

S. Babayeva, J. Nasibova, Z. Akparov, K. Shikhaliyeva, A. Mammadova, V. Izzatullayeva, M. Abbasov

{"title":"DNA标记在鹰嘴豆抗枯萎病及遗传多样性测定中的应用","authors":"S. Babayeva, J. Nasibova, Z. Akparov, K. Shikhaliyeva, A. Mammadova, V. Izzatullayeva, M. Abbasov","doi":"10.18805/LR-407","DOIUrl":null,"url":null,"abstract":"Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea\",\"authors\":\"S. Babayeva, J. Nasibova, Z. Akparov, K. Shikhaliyeva, A. Mammadova, V. Izzatullayeva, M. Abbasov\",\"doi\":\"10.18805/LR-407\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.\",\"PeriodicalId\":18181,\"journal\":{\"name\":\"Legume Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2018-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Legume Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.18805/LR-407\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.18805/LR-407","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 2

摘要

利用ISSR和RAPD对50个栽培鹰嘴豆基因型的遗传多样性和抗病性进行了研究。两种标记系统的结果一致，但rapd的结果略高。两种标记共获得48个片段，其中多态片段占32.7%。通过ISSR和RAPD数据进行分子特征分析，遗传变异低(GDI=0.39;本地鹰嘴豆和引种鹰嘴豆的PIC=0.21)。遗传距离为0 ~ 0.134，平均值为0.05。阿塞拜疆鹰嘴豆种质资源的遗传多样性较低，表明必须引入新的基因型来增加其多样性。通过对镰刀菌的抗性筛选，大多数基因型的OPJ20均与Foc01抗性基因有600 bp连锁，而对Foc4抗性基因均无连锁。研究结果可为今后鹰嘴豆的育种和保护提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Application of DNA markers in determination of fusarium resistance and genetic diversity in chickpea

Genetic diversity and fusarium resistance of 50 cultivated chickpea genotypes was studied using ISSR and RAPD markers. The results of both marker systems were consistent with each other, but was somewhat higher for RAPDs. A total of 48 fragments were obtained with both markers together, 32.7% of which were polymorphic. Molecular characterisation through ISSR and RAPD data indicated low genetic variation (GDI=0.39; PIC=0.21) among local and introduced chickpea accessions. The genetic distance values ranged from 0 to 0.134, with a mean of 0.05. The low genetic diversity indicated that new genotypes must be introduced to increase the variability of chickpea germplasm in Azerbaijan. As a result of screening for resistance to fusarium most of genotypes were found to have 600 bp fragment of OPJ20 linked with Foc01 resistance gene, while none of them had resistance gene to Foc4. The results could provide information for future chickpea breeding activities and conservation.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.