{"title":"tubingaspergillus tubingensis NIICC-08155本地分离株中性蛋白酶的生产及部分特性研究","authors":"V. Morya, D. Yadav","doi":"10.5580/1b13","DOIUrl":null,"url":null,"abstract":"An indigenously isolated strain of Aspergillus tubingensis NIICC-08155 from soil of teak dominated vegetation of kusmi forest at Gorakhpur was subjected to neutral protease production and its partial characterization. The maximum production of neutral protease i.e. 68.50 U/ml was attained after 96h of incubation. The crude preparation of protease showed reasonable activity at temperature range of 40 to 60 °C with maximum activity at 40 oC and had a maximum enzyme activity at pH 6.4. The Km value of the enzyme was found to be 45.0 mg/ml and the molecular weight as determined by SDS-PAGE was approximately 45 kDa. The enzyme was completely inhibited by DTT and EDTA.","PeriodicalId":22514,"journal":{"name":"The Internet journal of microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Production and partial characterization of neutral protease by an indigenously isolated strain of Aspergillus tubingensis NIICC-08155\",\"authors\":\"V. Morya, D. Yadav\",\"doi\":\"10.5580/1b13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An indigenously isolated strain of Aspergillus tubingensis NIICC-08155 from soil of teak dominated vegetation of kusmi forest at Gorakhpur was subjected to neutral protease production and its partial characterization. The maximum production of neutral protease i.e. 68.50 U/ml was attained after 96h of incubation. The crude preparation of protease showed reasonable activity at temperature range of 40 to 60 °C with maximum activity at 40 oC and had a maximum enzyme activity at pH 6.4. The Km value of the enzyme was found to be 45.0 mg/ml and the molecular weight as determined by SDS-PAGE was approximately 45 kDa. The enzyme was completely inhibited by DTT and EDTA.\",\"PeriodicalId\":22514,\"journal\":{\"name\":\"The Internet journal of microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Internet journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5580/1b13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Internet journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5580/1b13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Production and partial characterization of neutral protease by an indigenously isolated strain of Aspergillus tubingensis NIICC-08155
An indigenously isolated strain of Aspergillus tubingensis NIICC-08155 from soil of teak dominated vegetation of kusmi forest at Gorakhpur was subjected to neutral protease production and its partial characterization. The maximum production of neutral protease i.e. 68.50 U/ml was attained after 96h of incubation. The crude preparation of protease showed reasonable activity at temperature range of 40 to 60 °C with maximum activity at 40 oC and had a maximum enzyme activity at pH 6.4. The Km value of the enzyme was found to be 45.0 mg/ml and the molecular weight as determined by SDS-PAGE was approximately 45 kDa. The enzyme was completely inhibited by DTT and EDTA.
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