玉米麦秆配方和咖啡豆废弃物中白牡蛎菌丝的生长

Umrah Umrah, Eny Yuniati, Amiruddin Kasim, Kirana Kirana
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摘要

玉米秸秆和咖啡豆废料作为白平菇菌丝体生长所需的培养基和营养来源。这项研究是在Tadulako大学数学与自然科学学院生物系微生物实验室进行的。本研究的目的是测定白平菇(P. ostreatus)在玉米秸秆和咖啡豆废培养基中的菌丝生长情况及其特性。这项研究的目的是在一个完全随机设计(CRD)组成的七个治疗,三个复制,即P1(100%玉米秸秆粉),P2(95%玉米秸秆粉+ 5%咖啡豆浪费),P3(90%玉米秸秆粉+ 10%咖啡豆浪费),P4(85%玉米秸秆粉+ 15%咖啡豆浪费),P5(80%玉米秸秆粉+ 20%咖啡豆浪费),P6(75%玉米秸秆粉+ 25%咖啡豆浪费),P7(70%锯末+ 20%米糠+ 10%玉米面粉是一个积极的控制)。结果表明,以玉米秸秆和咖啡渣作为白平菇菌丝体生长培养基,菌丝体在各处理下均能良好生长,可作为基础培养基。P3处理的白平菇菌丝体生长速度很快,P6处理的菌丝体生长速度很慢。P3处理菌落数最多,为6.40 × 1011cfu /g, P6处理菌落数最少,为2.66 × 1011cfu /g。P3处理充瓶时间较短,为30天,而P6处理充瓶时间较长,为48天。
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PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI
Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.
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