利用病原菌的人工感染背景,培育具有复杂抗病能力的高产冬小麦品种

T. Mukha, L. Murashko, O. Humenyuk, Y. Suddenko, N. Novytska, O. Martynov
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引用次数: 0

摘要

大田作物病害大大降低了所获得农产品的产量和质量。每年因病虫害造成的粮食总产量选择不足可达25-30%。这就需要为冬小麦的选择创造出具有抗性的品种。本研究旨在为冬小麦选育提供一种对主要有害病害具有复合抗性的新型高产选育材料。根据e.e.h eschele的方法,在试管植株出苗期对冬小麦植株进行了褐锈病菌孢子侵染。为了创建一个人造的褐锈病感染背景,利用一个病原体的合成种群(国家农业科学院植物保护研究所)作为感染库——一个易感品种Myronivska 10。为了创建白粉病的人工感染背景,使用了当地人群和美国品种Keprock的感染库。在白粉病发生背景下的F3杂交苗圃中,选择了对病原菌高度抗性(高达5%)的组合,抗性来源为:Zdar、Fakon、Pi170911、Bongo - Svitanok Myronivskyi/Zdar、Kolos Myronivshchyny/Fakon、Berehynia Myronivska/Pi170911、Dostatok/Bongo。两个杂交组合(Gorlytsia Myronivska/Gloria和Remeslivna/Wervok)对病原菌的抗性最高达10.0%。以第4代杂交种Flex、V 1275、Tobarzo、203 ~ 238为抗褐锈病的人工感染背景,选择具有抗性来源的杂交组合。值得注意的是混合动力车:Oberih Myronivskyi/Flex, Smuhlianka/V 1275, monotype /Tobarzo, Kolos Myronivshchyna/203-238。单穗粒数以Svitanok Myronivskyi/Zdar最多(61.5粒),单穗粒重以Horlytsia Myronivska/Gloria和Berehynia Myronivska/Pi170911最多(分别为2.38 g和2.37 g)。Oberih Myronivskyi/Flex组合因其抗褐锈和性能元素而脱颖而出。根据研究结果选择的样品被用作在乌克兰森林草原培育抗病冬小麦新品种的宝贵原料
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CREATION OF HIGHLY PRODUCTIVE FORMS OF WINTER WHEAT WITH COMPLEX RESISTANCE AGAINST DISEASES AND PESTS ON ARTIFICIAL INFECTIOUS BACKGROUNDS OF THEIR CAUSATIVE AGENTS
Diseases of field crops substantially reduce the yield and quality of the obtained agricultural products. Underselection of gross grain harvest due to diseases and pests can reach about 25-30% annually. This necessitates the creation of resistant varieties for winter wheat selection. The purpose of this study was to create a new highly productive selection material with complex resistance against the main most harmful diseases for further use in the selection of winter wheat. In the phase of emergence of plants in the tube, winter wheat plants were infected with spores of the causative agent of brown rust according to E.E. Heschele’s method. To create an artificial infectious background of brown rust, a synthetic population of the pathogen (Institute of Plant Protection of the National Academy of Agrarian Sciences) was used as a reservoir of infection – a susceptible variety of Myronivska 10. To create an artificial infectious background of powdery mildew, a local population and an infectious reservoir of the American variety Keprock were used. In the F3 hybrid nursery against the provoking background of powdery mildew, combinations highly resistant to the pathogen (up to 5%) were selected, created with sources of resistance: Zdar, Fakon, Pi170911, Bongo – Svitanok Myronivskyi/Zdar, Kolos Myronivshchyny/Fakon, Berehynia Myronivska/Pi170911, Dostatok/Bongo. Resistance against the pathogen (up to 10.0%) was observed in two crossing combinations (Gorlytsia Myronivska/Gloria and Remeslivna/Wervok). Crossing combinations created with sources of resistance were selected on the artificial infectious background of hybrids of the fourth generation for resistance to brown rust: Flex, V 1275, Tobarzo, 203-238. It is worth noting the hybrids: Oberih Myronivskyi/Flex, Smuhlianka/V 1275, Monotyp/Tobarzo, Kolos Myronivshchyna/203-238. The largest number of grains in an ear was obtained from the combination Svitanok Myronivskyi/Zdar (61.5 pieces), and the largest weight of grain from an ear was obtained from the combinations Horlytsia Myronivska/Gloria and Berehynia Myronivska/Pi170911 (2.38 g and 2.37 g, respectively). The Oberih Myronivskyi/Flex combination stood out for its resistance to brown rust and performance elements. The samples selected based on the results of the study are used as valuable raw material to create new winter wheat varieties resistant to diseases in the Forest Steppe of Ukraine
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