圆草、羊蹄草、天竺葵、刺竹和巴豆提取物联合对环磷酰胺诱导的大鼠免疫抑制的免疫刺激作用

E. Chandana, Kankanam K. A. Kithmini, T. M. C. P. Gunasekara
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引用次数: 1

摘要

目的:验证香附根茎乙醇提取物的组合作用。(“Kalanduru”)和Alpinia calcarata Rosc。(" Heen Araththa "),以及天葵(Solanum surattense burm .)的根皮。(“Elabatu”),不幸的木结。(“Pinna”)和Croton laccifer Linn。(“Keppetiya”)作为大鼠免疫调节剂。方法:Wistar大鼠每天口服五种植物组合粗制品(200 mg/ml),连续14 d;对照组每天口服蒸馏水1 ml,连续14 d。测定各组大鼠治疗后白细胞(WBC)计数、白细胞粘附、IL-4、IL-10、IL-12水平。随后,每组动物口服浓度为20 mg/ml的环磷酰胺溶液1 ml。结果:血液学分析显示,对照组和提取物组的总白细胞计数和白细胞粘附无显著差异。治疗组与对照组IL-4、IL-10表达差异有统计学意义,IL-12表达差异无统计学意义。环磷酰胺诱导的免疫抑制对照组大鼠出现中度至重度皮肤损害,而提取物组大鼠未出现任何皮肤损害。环磷酰胺治疗组大鼠3个月后全部死亡,而环磷酰胺+植物提取物治疗组有83.33%的大鼠存活,说明植物组合能够缓解环磷酰胺诱导的免疫抑制。结论:上述植物乙醇提取物组合处理可能通过细胞因子的表达发挥免疫调节作用,并可减轻环磷酰胺诱导的免疫抑制。
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Immunostimulative effects of Cyperus rotundus, Alpinia calcarata, Solanum surattense, Clerodendrum infortunatum and Croton laccifer extracts combination on cyclophosphamide-induced immunosupression in rats -
Objective: The present work was undertaken to validate the combinatorial effect of the ethanol extractions of rhizomes of Cyperus rotundus Linn. (“Kalanduru”) and Alpinia calcarata Rosc. (“Heen Araththa”), and root barks of Solanum surattense Burm.f. (“Elabatu”), Clerodendrum infortunatum Linn. (“Pinna”) and Croton laccifer Linn. (“Keppetiya”) as immunomodulators in rats. Methods: Wistar rats were orally fed with combined crude products of the five plant species (200 mg/ml) at each day for a period of 14 days while control group was orally fed with 1 ml of distilled water each day for the same period. Differential white blood cell (WBC) count, leukocyte adhesion, IL-4, IL-10 and IL-12 levels were measured in all rats after the treatments. Subsequently, animals in each group were orally fed with 1 ml of cyclophosphamide solution at a concentration 20 mg/ml. Results: Hematological analyses revealed that total WBC and leukocyte adhesion were not significantly different in control and extract-treated groups. Expression of IL-4 and IL-10 was significantly different in treated and control groups while expression of IL-12 was not significantly different. Cyclophosphamide-induced immunosuppression of the control group caused moderate to severe skin lesions while the rats in the extract-treated group did not sustain any skin lesions. All the rats in the cyclophosphamide-treated control group died after three months while 83.33% of the cyclophosphamide + plant extract received group survived, indicating the ability of the plant combination to alleviate the immunosuppression induced by cyclophosphamide. Conclusions: Treating with ethanolic extract combination of above plant species might exert their immunomodulatory effect via cytokine expression and can attenuate the immunosuppression induced by cyclophosphamide.
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