Characterization and evaluation of putative mutant populations of polyembryonic mango genotype Nekkare for dwarfing rootstock traits

Availability of dwarfing rootstocks is an important pre-requisite for improving productivity of mango orchards in India as it facilitates high density planting as well as impart uniformity within an orchard. An attempt was made to induce variability in polyembryonic mango genotype Nekkare for dwarfness by treating kernels with different doses of gamma radiation ranging from 15 to 35 Gy. Irradiation created significant variation in plant height, stem girth, number of nodes, inter-nodal length, number of leaves, leaf blade length and leaf blade width. The highest reduction in seedling height along with highest variation was observed at 35 Gy where the seedling height ranged from 11.50 to 33 cm with a mean of 23.12 cm as compared to mean plant height of 44.55 cm in control ranging from 33.50 to 56 cm. Further, the effect of irradiation on stomatal parameters was also investigated and the highest stomatal length and width was recorded at 15 Gy (63.39 μm) and 20 Gy (63.12 μm) respectively while 30 Gy treatment produced maximum stomatal density (13.85 per μm2). Furthermore, theconcentration of ABA was found to be highest (429.1 ng/gm) in morphologically dwarf (putative mutant) progenies of Nekkare. The results suggest effectiveness of induced mutation for developing dwarfing rootstocks in mango to be used in high density planting.