正畸患者刷牙托槽时气溶胶吸入距离对链球菌和葡萄球菌存在的影响

Andi Triawan, Belladina Yusi Lasara
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摘要

牙医是感染传播风险最大的职业之一,因为牙科护理可能会产生许多细菌传播的感染性气溶胶。在实践中,使用符合牙医标准的个人防护装备和气溶胶吸盘对于防止患者交叉感染至关重要。本研究旨在确定正畸患者托槽刷牙过程中气溶胶吸入距离对总板数以及牙医面罩上链球菌和葡萄球菌存在情况的影响。使用的方法是牙医在刷牙正畸患者托槽后使用的面罩拭子法,气溶胶吸入到口腔的距离分别为10 cm、15 cm和20 cm。孵育2 × 24小时后,计算总平板计数,通过分析生长菌落特征,进行过氧化氢酶检测,鉴定链球菌和葡萄球菌。结果表明,当气溶胶吸入距离口腔10 cm时,面罩上的总板数最低,为1.23±0.01 CFU/cm2;单因素方差分析得到p < 0.05的显著性值,说明气溶胶吸入距离的变化影响总屏蔽板数。面罩上的细菌链球菌呈阴性,葡萄球菌呈阳性。
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The effect of aerosol suction distance during brushing brackets of orthodontic patients on the presence of Streptococcus and Staphylococcus
Dentists are one of the professions most at risk of infection transmission because dental care potentially produces many infectious aerosols for bacterial transmission. Using personal protective equipment and aerosol suction that meets the standards for dentists is vital to prevent cross-infection in patients in practice. This study aims to determine the effect of aerosol suction distance on the orthodontic patient bracket brushing process on total plate number and the presence of Streptococcus and Staphylococcus on the dentist’s face shield. The method used is the swab method on the face shield used by dentists after brushing orthodontic patient brackets with variations in the distance of aerosol suction to the oral cavity of 10 cm, 15 cm, and 20 cm. After 2 x 24 hours of incubation, the total plate count was calculated, and Streptococcus and Staphylococcus were identified by analyzing the characteristics of the growing colonies and executing the catalase test. The results showed that the lowest total plate number found on a face shield with aerosol suction distance of 10 cm from the oral cavity, 1.23 ± 0.01 CFU/cm2. The one-way ANOVA test obtained a significance value of p < 0.05, concluding that the variation of the aerosol suction distance affects the total face shield plate number. The presence of bacteria on the face shield was negative for Streptococcus and positive for Staphylococcus.
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