Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.76176
D. Agustina, Regina Titi Chrisnawati, Bernadetta Esti Chrismawaty, Sri Budiarti Wongsohardjono, Fimma Naritasari, Andari Sarasati
Patients with diabetes are prone to recurring and even resistant Candidiasis, making treatment challenging. Many hypotheses proposed related to susceptibility of diabetic patients to Candida. The prevalence and species of Candida in a particular diabetic community might be different compared to other diabetic community. This study aimed to determine the prevalence of Candida colony and its species in the oral cavity of diabetic patients included in the CDM (Chronic Disease Management) program in Yogyakarta. One hundred patients with type 2 diabetes (n= 100) were recruited as the subjects of this study. The subjects were classified into controlled and uncontrolled Diabetes mellitus (DM). Samples of oral rinse solution were collected to determine the species of Candida and number of Candida colonies using CHROMagar Candida medium. There were 47 and 53 of subjects with controlled and uncontrolled DM, respectively. The mean number of Candida colony in the subjects with controlled diabetes (1003.13) was higher than that in the subjects with uncontrolled diabetes (478.43). The Candida colony most commonly identified in the subjects with controlled and uncontrolled diabetes were C. albicans and C. glabrata, respectively. Female patients had higher mean number of Candida colony (859.51) compared to male (299.21). The Candida colony most often identified in both genders was C. albicans. In addition, the subjects of this study consisted of 83 geriatric subjects and 17 non geriatric subjects, in which the mean number of Candida colony in the geriatric subjects (761.77) was higher than that in the non-geriatric subjects (545.71). The Candida colony most often identified in the geriatric subjects and non-geriatric subjects was C. albicans and C. glabrata, respectively. The Mann Whitney test demonstrated that there was a significant difference (p = 0.009) of the mean number of Candida colony between male and female. However, there was no significant difference of the mean number of Candida colony between ages (p = 0.060) and diabetic status (p=0.175). It can be concluded that the Candida species most commonly identified in all the subjects was Candida albicans with the mean colony number of 349.96, followed by C. glabrata (225.97), C. krusei (144.91), C. tropicalis (3.67), and other species (2.02).
{"title":"Prevalence and identification of oral candida species in patients with type 2 diabetes in Yogyakarta","authors":"D. Agustina, Regina Titi Chrisnawati, Bernadetta Esti Chrismawaty, Sri Budiarti Wongsohardjono, Fimma Naritasari, Andari Sarasati","doi":"10.22146/majkedgiind.76176","DOIUrl":"https://doi.org/10.22146/majkedgiind.76176","url":null,"abstract":"Patients with diabetes are prone to recurring and even resistant Candidiasis, making treatment challenging. Many hypotheses proposed related to susceptibility of diabetic patients to Candida. The prevalence and species of Candida in a particular diabetic community might be different compared to other diabetic community. This study aimed to determine the prevalence of Candida colony and its species in the oral cavity of diabetic patients included in the CDM (Chronic Disease Management) program in Yogyakarta. One hundred patients with type 2 diabetes (n= 100) were recruited as the subjects of this study. The subjects were classified into controlled and uncontrolled Diabetes mellitus (DM). Samples of oral rinse solution were collected to determine the species of Candida and number of Candida colonies using CHROMagar Candida medium. There were 47 and 53 of subjects with controlled and uncontrolled DM, respectively. The mean number of Candida colony in the subjects with controlled diabetes (1003.13) was higher than that in the subjects with uncontrolled diabetes (478.43). The Candida colony most commonly identified in the subjects with controlled and uncontrolled diabetes were C. albicans and C. glabrata, respectively. Female patients had higher mean number of Candida colony (859.51) compared to male (299.21). The Candida colony most often identified in both genders was C. albicans. In addition, the subjects of this study consisted of 83 geriatric subjects and 17 non geriatric subjects, in which the mean number of Candida colony in the geriatric subjects (761.77) was higher than that in the non-geriatric subjects (545.71). The Candida colony most often identified in the geriatric subjects and non-geriatric subjects was C. albicans and C. glabrata, respectively. The Mann Whitney test demonstrated that there was a significant difference (p = 0.009) of the mean number of Candida colony between male and female. However, there was no significant difference of the mean number of Candida colony between ages (p = 0.060) and diabetic status (p=0.175). It can be concluded that the Candida species most commonly identified in all the subjects was Candida albicans with the mean colony number of 349.96, followed by C. glabrata (225.97), C. krusei (144.91), C. tropicalis (3.67), and other species (2.02).","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72880140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.81482
S. Suryono, Arifia Anindita Danastri
Injectable platelet-rich fibrin (i-PRF) refers to second-generation platelet concentrate. In this study, the results of i-PRF centrifugation were fractionated into three layers: yellow i-PRF, buffy coat, and red i-PRF. Injectable platelet-rich fibrin fractions used in this study were yellow i-PRF, red i-PRF, and a mix of both. This study aimed to examine the level of growth factor release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor (PDGF) in yellow i-PRF, red i-PRF, and a mix of yellow i-PRF and red i-PRF with the ratio of 1:1. A total of 10 ml of peripheral blood from healthy female donors was centrifuged (at 700 rpm in 3 minutes) to obtain i-PRF and fractionated into three layers. The upper yellow layer was taken as yellow i-PRF, while the bottom red layer was taken as red i-PRF and was taken together with the middle layer (buffy coat). The release of TGF-β1 and PDGF in each of i-PRF fractionation method, i.e. yellow i-PRF, red i-PRF, and a mix of yellow i-PRF and red i-PRF with a ratio of 1:1 was measured with ELISA. The measurement was observed for 24 hours, 3 days, 7 days, 10 days, and 14 days. Data analysis used the two-way ANOVA test with a significance level of 0.05 and a post hoc LSD analysis to establish group significance. The group of yellow + red i-PRF significantly released PDGF-AB (p < 0.05). TGF-β1 was the highest of all groups on day 14. All groups showed an increase in growth factor release from time to time. The fractionation method of injectable platelet-rich fibrin affected the release of growth factor of PDGF-AB and TGF-β1. The highest release of PDGF-AB and TGF-β1 was found in the yellow + red i-PRF group with a ratio of 1:1 in the 14-day group, which was significant with the other two groups (p < 0.05).
{"title":"Differences in injectable platelet-rich fibrin fraction of peripheral blood on the release of TGF-β1 and PDGF-AB","authors":"S. Suryono, Arifia Anindita Danastri","doi":"10.22146/majkedgiind.81482","DOIUrl":"https://doi.org/10.22146/majkedgiind.81482","url":null,"abstract":"Injectable platelet-rich fibrin (i-PRF) refers to second-generation platelet concentrate. In this study, the results of i-PRF centrifugation were fractionated into three layers: yellow i-PRF, buffy coat, and red i-PRF. Injectable platelet-rich fibrin fractions used in this study were yellow i-PRF, red i-PRF, and a mix of both. This study aimed to examine the level of growth factor release of transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor (PDGF) in yellow i-PRF, red i-PRF, and a mix of yellow i-PRF and red i-PRF with the ratio of 1:1. A total of 10 ml of peripheral blood from healthy female donors was centrifuged (at 700 rpm in 3 minutes) to obtain i-PRF and fractionated into three layers. The upper yellow layer was taken as yellow i-PRF, while the bottom red layer was taken as red i-PRF and was taken together with the middle layer (buffy coat). The release of TGF-β1 and PDGF in each of i-PRF fractionation method, i.e. yellow i-PRF, red i-PRF, and a mix of yellow i-PRF and red i-PRF with a ratio of 1:1 was measured with ELISA. The measurement was observed for 24 hours, 3 days, 7 days, 10 days, and 14 days. Data analysis used the two-way ANOVA test with a significance level of 0.05 and a post hoc LSD analysis to establish group significance. The group of yellow + red i-PRF significantly released PDGF-AB (p < 0.05). TGF-β1 was the highest of all groups on day 14. All groups showed an increase in growth factor release from time to time. The fractionation method of injectable platelet-rich fibrin affected the release of growth factor of PDGF-AB and TGF-β1. The highest release of PDGF-AB and TGF-β1 was found in the yellow + red i-PRF group with a ratio of 1:1 in the 14-day group, which was significant with the other two groups (p < 0.05).","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83908190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.78246
Diana Soesilo, Fani Pangabdian, Eriza Juniar
Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis.
{"title":"Antibiofilm activity of Cyanobacteria spirulina as an irrigation solution against Enterococcus faecalis","authors":"Diana Soesilo, Fani Pangabdian, Eriza Juniar","doi":"10.22146/majkedgiind.78246","DOIUrl":"https://doi.org/10.22146/majkedgiind.78246","url":null,"abstract":"Sodium hypochlorite (NaOCl) is currently the golden standard for root canal irrigation. NaOCl at a concentration of 5.25% to 6% can eliminate E. faecalis, but this concentration can increase the risk of toxic effects. Cyanobacteria spirulina is known to produce several secondary metabolites that have antimicrobial activity against gram-positive and gram-negative bacteria. The aim of this study was to determine the antibiofilm power of Cyanobacteria spirulina against the biofilms of Enterococcus faecalis at concentrations of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml. This research was a true experiment with a post-test only group design. The object of the research was divided into 6 groups. Group 1 was a negative control group, group 2 was a positive control group with 5% NaOCl, group 3, 4, 5, 6 were treatment groups given Cyanobacteria spirulina solution at a concentration of 60 mg/ml, 70 mg/ml, 80 mg/ml, and 90 mg/ml, respectively. Optical density (OD) of bacteria was bound by staining and analyzed by ELISA auto reader with a wave length of 595 nm (OD 595 nm). The results of the LSD test showed that the significance between K+ and P1, P2, P3 (p< 0.005), K+ and P4 (p= 0.129), P1 and P2 (p=0.449), P3 and P4 (p=0.178). Significance of p<0.0005 showed a significant difference between the groups. The data were analyzed using the one-way ANOVA test followed by a double comparison test with the Least Significance Different (LSD) Post Hoc test method. Cyanobacteria spirulina solution at a concentration of 90 mg/ml had the greatest inhibitory effect on the biofilm formation of Enterococcus faecalis.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"65 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84271647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.80060
S. Sunarintyas, D. Irnawati, Harsini Harsini, M. Rinastiti, N. Nuryono
Synthetic dental fiber tape for fiber reinforcement (FRC) restoration is relatively costly and its availability is still limited in Indonesia, so natural dental fibers have been used as an alternative material. The aim of this study was to assess the effect of impregnation of various fiber tape toward the flexural strength and hardness of FRC. The materials used were natural Bombyx mori silk fibers (Indonesia), dental polyethylene tape (Construct Kerr, USA), dental Quartz tape (Quartz Splint UD, France), dental E-glass tape (Everstick TM, GC, Japan), silane coupling agent (Ultradent, Jordan), and composite resin (Denfil-Flow, USA). Five groups of samples consisted of FRC with various fiber tapes were prepared: unidirectional-silk, braided-silk, quartz, polyethylene, and E-glass. The five groups of FRC were tested to determine the flexural strength and hardness. The results were analyzed by one-way ANOVA, followed by LSD test. The results showed that the highest flexural strength was in the quartz group (496.84 ± 109.14 MPa), while the lowest was in the braided-silk group (139.39 ± 4.30 MPa). The highest hardness property was in the unidirectional-silk group (141.29 ± 25.17 VHN), while the lowest was in the braided-silk group (139.39 ± 4.30 VHN). The ANOVA showed that various fiber tapes significantly influenced the flexural strength and hardness of FRC (p < 0.05). The LSD showed that the unidirectional-silk, braided-silk, and polyethylene groups demonstrated no significant difference. The LSD for hardness showed that the unidirectional-silk group had a significant difference with the other groups (p < 0.05). It can be concluded that various fiber tapes influenced the flexural strength and hardness of FRC. Natural silk fibers showed comparable flexural strength and hardness with the other fiber tapes.
{"title":"Impregnation of various fiber tapes toward mechanical properties of dental fiber-reinforced composites","authors":"S. Sunarintyas, D. Irnawati, Harsini Harsini, M. Rinastiti, N. Nuryono","doi":"10.22146/majkedgiind.80060","DOIUrl":"https://doi.org/10.22146/majkedgiind.80060","url":null,"abstract":"Synthetic dental fiber tape for fiber reinforcement (FRC) restoration is relatively costly and its availability is still limited in Indonesia, so natural dental fibers have been used as an alternative material. The aim of this study was to assess the effect of impregnation of various fiber tape toward the flexural strength and hardness of FRC. The materials used were natural Bombyx mori silk fibers (Indonesia), dental polyethylene tape (Construct Kerr, USA), dental Quartz tape (Quartz Splint UD, France), dental E-glass tape (Everstick TM, GC, Japan), silane coupling agent (Ultradent, Jordan), and composite resin (Denfil-Flow, USA). Five groups of samples consisted of FRC with various fiber tapes were prepared: unidirectional-silk, braided-silk, quartz, polyethylene, and E-glass. The five groups of FRC were tested to determine the flexural strength and hardness. The results were analyzed by one-way ANOVA, followed by LSD test. The results showed that the highest flexural strength was in the quartz group (496.84 ± 109.14 MPa), while the lowest was in the braided-silk group (139.39 ± 4.30 MPa). The highest hardness property was in the unidirectional-silk group (141.29 ± 25.17 VHN), while the lowest was in the braided-silk group (139.39 ± 4.30 VHN). The ANOVA showed that various fiber tapes significantly influenced the flexural strength and hardness of FRC (p < 0.05). The LSD showed that the unidirectional-silk, braided-silk, and polyethylene groups demonstrated no significant difference. The LSD for hardness showed that the unidirectional-silk group had a significant difference with the other groups (p < 0.05). It can be concluded that various fiber tapes influenced the flexural strength and hardness of FRC. Natural silk fibers showed comparable flexural strength and hardness with the other fiber tapes.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81979729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.80913
Tunjung Nugraheni, Yulianasari Yulianasari, Lia Desire Octarina
Dental trauma mostly damages the maxillary anterior teeth, affecting the individual’s esthetics, functionality, and psychological well-being. Restorative dentistry treatment is required to obtain a natural appearance based on the smile design approach. The aim of this report is to characterize the procedure of complicated cosmetic restoration of the six maxillary anterior teeth, which results in straighter and more beautiful natural-looking smiles. A 32-year-old male patient presented with multiple crown fractures in the anterior maxillary teeth, resulting in an unaesthetics smile. The patient also had a deep bite. A comprehensive aesthetics dental treatment was carried out. The dental treatment plan had to be carefully executed by considering several factors such as dental convexities and contours, re‑establishment of function, and esthetics. Smile design should always be approached as a multi-factor decision-making, which enables the doctor to treat patients using a personalized and interdisciplinary approach.
{"title":"Complex aestheticss treatment of six maxillary anterior teeth with smile design and deep bite correction","authors":"Tunjung Nugraheni, Yulianasari Yulianasari, Lia Desire Octarina","doi":"10.22146/majkedgiind.80913","DOIUrl":"https://doi.org/10.22146/majkedgiind.80913","url":null,"abstract":"Dental trauma mostly damages the maxillary anterior teeth, affecting the individual’s esthetics, functionality, and psychological well-being. Restorative dentistry treatment is required to obtain a natural appearance based on the smile design approach. The aim of this report is to characterize the procedure of complicated cosmetic restoration of the six maxillary anterior teeth, which results in straighter and more beautiful natural-looking smiles. A 32-year-old male patient presented with multiple crown fractures in the anterior maxillary teeth, resulting in an unaesthetics smile. The patient also had a deep bite. A comprehensive aesthetics dental treatment was carried out. The dental treatment plan had to be carefully executed by considering several factors such as dental convexities and contours, re‑establishment of function, and esthetics. Smile design should always be approached as a multi-factor decision-making, which enables the doctor to treat patients using a personalized and interdisciplinary approach.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78052224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To evaluate the effect of eucalyptus, sappan, and cinnamon leaf and bark extract on the percentage of S. mutans biofilm formation. The test group was divided into a negative control (1% Dimethyl sulfoxide), a treatment group (eucalyptus, sappan, and cinnamon leaves and bark at concentrations of 50%, 25%, and 12.5%, respectively), and a positive control (0.2% chlorhexidine). Each concentration of eucalyptus, sappan, and cinnamon leaves and barks extract, Brain Heart Infusion Broth (BHI-B), bacteria according to the McFarland 0.5 standard, a positive control, and a negative control were added to a 96-well microplate. They were incubated at 37 °C for twenty-four hours before being rinsed with phosphate buffer saline (PBS) and stained with 0.1% crystal violet. The optical density was then measured using a microplate reader with a 540 nm wavelength. The absorbance value is then factored into the percentage of bacterial adhesion inhibition formula. A statistical test revealed a significant difference (p < 0.05) in the percentage of inhibition between the three extracts of leaves and bark and the negative control group (1% DMSO). There is a significant difference between all leaves and barks concentration and the positive control group except the 50% concentration of sappan leaf group and 50% concentration of cinnamon leaf group. The sappan leaf and cinnamon leaf at 50% concentration exhibit no significant difference (p > 0.05) with the positive control. Eucalyptus, sappan, cinnamon leaves and barks at a concentration of 50% demonstrated effectiveness of the extractant in inhibiting the formation of biofilm masses by S. mutans in comparison with the other group.
{"title":"Comparison Effect of Leaves and Bark Extract of Eucalyptus (Melaleuca leucadendra), Sappan (Caesalpinia sappan), and Cinnamon (Cinnamomum zeylanicum) to Reduce Streptococcus mutans Biofilm Formation","authors":"Trianna Wahyu Utami, Bernadetha Nathania Ekananda, Yasmin Regita Anjani, Dyah Listyarifah, Asikin Nur, A. Syahbudin","doi":"10.22146/majkedgiind.83283","DOIUrl":"https://doi.org/10.22146/majkedgiind.83283","url":null,"abstract":"To evaluate the effect of eucalyptus, sappan, and cinnamon leaf and bark extract on the percentage of S. mutans biofilm formation. The test group was divided into a negative control (1% Dimethyl sulfoxide), a treatment group (eucalyptus, sappan, and cinnamon leaves and bark at concentrations of 50%, 25%, and 12.5%, respectively), and a positive control (0.2% chlorhexidine). Each concentration of eucalyptus, sappan, and cinnamon leaves and barks extract, Brain Heart Infusion Broth (BHI-B), bacteria according to the McFarland 0.5 standard, a positive control, and a negative control were added to a 96-well microplate. They were incubated at 37 °C for twenty-four hours before being rinsed with phosphate buffer saline (PBS) and stained with 0.1% crystal violet. The optical density was then measured using a microplate reader with a 540 nm wavelength. The absorbance value is then factored into the percentage of bacterial adhesion inhibition formula. A statistical test revealed a significant difference (p < 0.05) in the percentage of inhibition between the three extracts of leaves and bark and the negative control group (1% DMSO). There is a significant difference between all leaves and barks concentration and the positive control group except the 50% concentration of sappan leaf group and 50% concentration of cinnamon leaf group. The sappan leaf and cinnamon leaf at 50% concentration exhibit no significant difference (p > 0.05) with the positive control. Eucalyptus, sappan, cinnamon leaves and barks at a concentration of 50% demonstrated effectiveness of the extractant in inhibiting the formation of biofilm masses by S. mutans in comparison with the other group.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89658371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.77132
Dian Praba Ramadhanti, Eka Prasasti Nur Rachmani, A. Kurniawan
Denture stomatitis is an infection of the mucosa caused by bacteria such as Staphylococcus aureus (S. aureus) accumulating on the denture. Garlic (Allium sativum) contains antibacterial compounds that can be used as an alternative denture cleanser. The purpose of this study was to determine the inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum) against Staphylococcus aureus growth on heat cured acrylic dental plate. This research was a laboratory experiment in vitro using 40%, 50%, and 60% effervescent garlic ethanol extract. The samples were 12 pieces (n = 12) of heat cured acrylic plates which were divided into 3 treatment groups then incubated in S. aureus suspension for 24 hours and then immersed in an effervescent garlic ethanol extract for 6 hours. Bacterial colonies were counted using a colony counter and the data were analyzed using the One-way ANOVA and LSD Post hoc tests. The statistical analysis showed that the number of S. aureus colonies decreased along with an increased concentration of garlic ethanol extract. The results of the analysis showed that the 60% effervescent garlic ethanol extract had a significantly lower mean number of colonies compared to the 40% and 50% effervescent garlic ethanol extracts (p ≤ 0.05). This research concluded that effervescent garlic ethanol extract prevented the growth of S. aureus on the heat cured acrylic dental plate.
{"title":"An Inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum L.) against Staphylococcus aureus growth on heat cured acrylic","authors":"Dian Praba Ramadhanti, Eka Prasasti Nur Rachmani, A. Kurniawan","doi":"10.22146/majkedgiind.77132","DOIUrl":"https://doi.org/10.22146/majkedgiind.77132","url":null,"abstract":"Denture stomatitis is an infection of the mucosa caused by bacteria such as Staphylococcus aureus (S. aureus) accumulating on the denture. Garlic (Allium sativum) contains antibacterial compounds that can be used as an alternative denture cleanser. The purpose of this study was to determine the inhibition effect of immersion in effervescent garlic ethanol extract (Allium sativum) against Staphylococcus aureus growth on heat cured acrylic dental plate. This research was a laboratory experiment in vitro using 40%, 50%, and 60% effervescent garlic ethanol extract. The samples were 12 pieces (n = 12) of heat cured acrylic plates which were divided into 3 treatment groups then incubated in S. aureus suspension for 24 hours and then immersed in an effervescent garlic ethanol extract for 6 hours. Bacterial colonies were counted using a colony counter and the data were analyzed using the One-way ANOVA and LSD Post hoc tests. The statistical analysis showed that the number of S. aureus colonies decreased along with an increased concentration of garlic ethanol extract. The results of the analysis showed that the 60% effervescent garlic ethanol extract had a significantly lower mean number of colonies compared to the 40% and 50% effervescent garlic ethanol extracts (p ≤ 0.05). This research concluded that effervescent garlic ethanol extract prevented the growth of S. aureus on the heat cured acrylic dental plate.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76087036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.82711
M. Rinastiti, Andina Widyastuti, W. Hadriyanto
Preparation of the root canal system is a crucial step in root canal treatment. Endodontically treated teeth differ structurally from healthy, untreated teeth. This can lead to root cracking by creating pressure on the canal wall, reducing the fracture resistance of the tooth. Different designs, including cross-sectional shape, tip, taper, flute, radial land, helix angle, rake angle, and pitch, can influence the outcome of the root canal preparation and the risk of root fracture. The purpose of this study was to evaluate the fracture resistance of teeth after root canal preparation using three different NiTi rotary files: ProTaper Next (PN), ProTaper Gold (PG), and HyFlex CM (HC). Thirty premolars with a single and straight root canal were decoronated at the cementoenamel junction, leaving 14 mm of the root. The subjects were divided into 3 groups. Group 1 (n = 10) was prepared using PN, group 2 (n = 10) was prepared using PG, and group 3 (n = 10) was prepared using HC. After instrumentation and irrigation, the specimens were subjected to a continuous vertical compressive force (crosshead-speed of 2.28 mm/sec) in order to record the force (in newtons) until root fracture. To describe the surface characteristic of the dentinal root after the preparation, a section fragment from the apical third of the specimens was observed using a scanning electron microscope (SEM) at 2500x magnification. The micrographs were analyzed according to the Hulsman’s method. The results demonstrated that there was a statistically significant difference in root fracture resistance among the three groups. (p = 0.043). The LSD post hoc test indicated that HC showed a higher root fracture resistance (p < 0.05) compared to both PN and PG. In conclusion, the different file systems of PN, PG, and HC instruments generate different root fracture resistance of teeth. In comparison to PN and PG, HC instruments tend to result in increased fracture resistance.
根管系统准备是根管治疗的关键步骤。牙髓治疗后的牙齿在结构上与未治疗的健康牙齿不同。这会对根管壁造成压力，从而导致牙根开裂，降低牙齿的抗折能力。不同的设计，包括横截面形状、尖端、锥度、凹槽、径向地、螺旋角、前角和节距，会影响根管准备的结果和根管断裂的风险。本研究的目的是评估使用三种不同的NiTi旋转锉:ProTaper Next (PN)、ProTaper Gold (PG)和HyFlex CM (HC)进行根管预备后牙齿的抗折断性。在牙髓-牙釉质交界处装饰30颗具有单一直根管的前磨牙，留下14mm的根管。受试者分为3组。第1组(n = 10)采用PN制备，第2组(n = 10)采用PG制备，第3组(n = 10)采用HC制备。在仪器和灌溉后，试件连续承受垂直压缩力(十字头速度为2.28 mm/sec)，以记录力(单位为牛顿)直至根部断裂。为了描述制备后牙本质根的表面特征，使用2500倍放大的扫描电子显微镜(SEM)观察了标本根尖三分之一的切片碎片。显微照片按赫尔斯曼法进行分析。结果显示，三组患者牙根抗折性差异有统计学意义。(p = 0.043)。LSD事后检验显示，HC比PN和PG具有更高的牙根抗折能力(p < 0.05)。由此可见，不同文件系统的PN、PG和HC器械对牙齿牙根抗折能力的影响是不同的。与PN和PG相比，HC器械倾向于增加抗骨折能力。
{"title":"Comparison of fracture resistance of teeth with prepared protaper next, protaper gold, and hyflex CM rotary files","authors":"M. Rinastiti, Andina Widyastuti, W. Hadriyanto","doi":"10.22146/majkedgiind.82711","DOIUrl":"https://doi.org/10.22146/majkedgiind.82711","url":null,"abstract":"Preparation of the root canal system is a crucial step in root canal treatment. Endodontically treated teeth differ structurally from healthy, untreated teeth. This can lead to root cracking by creating pressure on the canal wall, reducing the fracture resistance of the tooth. Different designs, including cross-sectional shape, tip, taper, flute, radial land, helix angle, rake angle, and pitch, can influence the outcome of the root canal preparation and the risk of root fracture. The purpose of this study was to evaluate the fracture resistance of teeth after root canal preparation using three different NiTi rotary files: ProTaper Next (PN), ProTaper Gold (PG), and HyFlex CM (HC). Thirty premolars with a single and straight root canal were decoronated at the cementoenamel junction, leaving 14 mm of the root. The subjects were divided into 3 groups. Group 1 (n = 10) was prepared using PN, group 2 (n = 10) was prepared using PG, and group 3 (n = 10) was prepared using HC. After instrumentation and irrigation, the specimens were subjected to a continuous vertical compressive force (crosshead-speed of 2.28 mm/sec) in order to record the force (in newtons) until root fracture. To describe the surface characteristic of the dentinal root after the preparation, a section fragment from the apical third of the specimens was observed using a scanning electron microscope (SEM) at 2500x magnification. The micrographs were analyzed according to the Hulsman’s method. The results demonstrated that there was a statistically significant difference in root fracture resistance among the three groups. (p = 0.043). The LSD post hoc test indicated that HC showed a higher root fracture resistance (p < 0.05) compared to both PN and PG. In conclusion, the different file systems of PN, PG, and HC instruments generate different root fracture resistance of teeth. In comparison to PN and PG, HC instruments tend to result in increased fracture resistance.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84730923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.77682
A. Primasari, Yendriwati Yendriwati, Minasari Minasari, Erlin Kristiani Buaya, Sri Ayu Winda Madani, Kartika Nabila Sembiring, Rizka Hidayati
Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.
{"title":"Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats","authors":"A. Primasari, Yendriwati Yendriwati, Minasari Minasari, Erlin Kristiani Buaya, Sri Ayu Winda Madani, Kartika Nabila Sembiring, Rizka Hidayati","doi":"10.22146/majkedgiind.77682","DOIUrl":"https://doi.org/10.22146/majkedgiind.77682","url":null,"abstract":"Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82229399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-01DOI: 10.22146/majkedgiind.69468
A. Nuryanti, P. Larasati, Alda Risma Almeyda, Monica Agnesia Marandof, Catherine Marcia Irawan
Gingivitis is the second most common disease that occurs in the oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such as allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, and tannins compounds reported to have an influence on wound healing process. The aim of this research was to determine the effect of observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract on the number of neutrophils, macrophages, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rats. Sixty male Sprague Dawley rats aged 2.5-3 months with body weight of 200-250 g were anesthetized with ketamine HCl (10 mg/kg BW) and xylazine (2 mg/kg BW) then induced using silk ligature 3.0 on the interdental of the mandibular incisor. The rats were divided into five groups and got daily spray using distilled water (negative control), nano-extract at a concentration of 40%, 45%, 50%, and 0.12% chlorhexidine (positive control). The rats were euthanized on the 1st, 3rd, 5th, and 7th days post-treatment. The tissues were processed histologically with HE staining. The number of neutrophils, macrophages, and angiogenesis were counted using a microscope (400x) and OptiLab Viewer® (13x) at five fields of view. The result of the Two Way ANOVA test showed that there were significant effect of concentrations and observation time, and interaction of both observation time and concentrations on the number of neutrophils, macrophages, and angiogenesis (p < 0.05). It can be concluded that observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract affected the number of neutrophils, macrophage, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rat (p < 0.05).
{"title":"A potential of Jasminum sambac (L.) Aiton leaf nano-extract as spray treatment of gingivitis-induced Sprague Dawley rats","authors":"A. Nuryanti, P. Larasati, Alda Risma Almeyda, Monica Agnesia Marandof, Catherine Marcia Irawan","doi":"10.22146/majkedgiind.69468","DOIUrl":"https://doi.org/10.22146/majkedgiind.69468","url":null,"abstract":"Gingivitis is the second most common disease that occurs in the oral cavity. The use of chlorhexidine as a gingivitis therapy agent has some side effects such as allergic reactions and bacterial resistance. Jasminum Sambac (L.) Aiton leaf nano-extract contains flavonoids, saponins, and tannins compounds reported to have an influence on wound healing process. The aim of this research was to determine the effect of observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract on the number of neutrophils, macrophages, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rats. Sixty male Sprague Dawley rats aged 2.5-3 months with body weight of 200-250 g were anesthetized with ketamine HCl (10 mg/kg BW) and xylazine (2 mg/kg BW) then induced using silk ligature 3.0 on the interdental of the mandibular incisor. The rats were divided into five groups and got daily spray using distilled water (negative control), nano-extract at a concentration of 40%, 45%, 50%, and 0.12% chlorhexidine (positive control). The rats were euthanized on the 1st, 3rd, 5th, and 7th days post-treatment. The tissues were processed histologically with HE staining. The number of neutrophils, macrophages, and angiogenesis were counted using a microscope (400x) and OptiLab Viewer® (13x) at five fields of view. The result of the Two Way ANOVA test showed that there were significant effect of concentrations and observation time, and interaction of both observation time and concentrations on the number of neutrophils, macrophages, and angiogenesis (p < 0.05). It can be concluded that observation time and concentrations of Jasminum sambac (L.) Aiton leaf nano-extract affected the number of neutrophils, macrophage, and angiogenesis in the treatment of gingivitis-induced Sprague Dawley rat (p < 0.05).","PeriodicalId":31262,"journal":{"name":"Majalah Kedokteran Gigi Indonesia","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79562470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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