不同剂量HCG对花椰菜人工繁殖的影响

S. Lai, T. Le
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摘要

不同剂量HCG对侏儒gourus人工繁殖的试验目的是鉴定地鼠(Trichogaster lalius)的成熟能力,比较不同剂量HCG对侏儒gourus繁殖效率的影响。试验分为4个处理(NT): 3000UI(NT1)、4000UI(NT2)、5000UI(NT3)、6000UI/kg(NT4)雌性。每次治疗重复3次。结果表明,在调养过程中,温度、pH等环境因子均在适宜的范围内。成熟率最高的是雌虫40%,雄虫45.83%。调节过程的成活率很低,雄性为88.33%,雌性为66.67%,但不影响实验结果。不同剂量HCG刺激生殖,受精率在33.33% ~ 66.67%之间波动,受精率为268.56 ~ 389.72个卵/ g,受精率为61.50%,孵化率为98.71%。花椰菜最适宜的HCG用量为4000UI, 4个处理的最高育性为389.72个卵/g雌。
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EXPERIMENT ON ARTIFICIAL REPRODUCTION OF BROCCOLI (Trichogaster Lalius) WITH HCG AT DIFFERENT DOSAGES
The trail on artificial reproduction of dwarf gourami(Trichogaster lalius) with HCG at different dosages is to identify the maturation ability of Gophers (Trichogaster lalius) and compare the influence of HCG at different doses on the reproductive efficiency of dwarf gourami.The experiment consisted of 4  treatments (NT): 3000UI(NT1), 4000UI(NT2), 5000UI(NT3), 6000UI/kg(NT4) females. Each treatment was repeated 3 times. The results showed that during the  conditioning process, environmental factors such as temperature and pH were in the appropriate range for the maturation process of broodstock. The highest maturation rate is 40% in females and 45.83% in males. The survival rate in the conditioning process was quite low at 88.33% in males and 66.67% in females,  which however did not affect the results of the experiment. For stimulation to reproduction by HCG at different doses, the fertility rate fluctuates from 33.33% - 66.67% fertility ability ranges from 268.56 to 389.72 eggs / g female, fertilization rate 61.50%, and hatching rate 98.71%. The most appropriate dose of HCG for  broccoli is 4000UI and the highest fertility of the four treatments is 389.72 eggs/g females.
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