DNA附着在光学捕获的微结构珠上,由珠位移监测

J Dapprich, N Nicklaus
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引用次数: 16

摘要

可逆结合硅胶盒已开发形成反应容器,其中“单分子化学”可以执行。我们使用光学陷阱驱动1μm链亲和素包被的珠子进入含有生物素化DNA的区域,直到DNA链结合发生。在反射模式下使用象限检测器来跟踪被困珠的横向位置。微球和溶液之间的相对运动产生粘性阻力,当单链DNA附着在微球上时,这种阻力会增加;DNA头的附着可以在几分钟内完成,只需要不到一飞摩尔的DNA。该方法允许对单分子消化进行研究。
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DNA attachment to optically trapped beads in microstructures monitored by bead displacement

Reversibly binding silicone cartridges have been developed to form reaction containers in which ‘single molecule chemistry’ can be performed. We use an optical trap to drive 1μm streptavidin-coated beads into a region containing biotinylated DNA until binding of a strand of DNA occurs. A quadrant detector is used in reflective mode to track the lateral position of trapped beads. Relative motion between the bead and the solution causes a viscous drag force which is increased when a single strand of DNA is attached to the bead; DNA-bead attachment is done in minutes with less than a femtomole of DNA. The method allows the study of single molecule digestion.

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