Benalu Tea提取物对Hella细胞的细胞毒性作用

Kusumo Hariyadi, F. Fatmawati, Subandrate Subandrate, Fahira Aninditia, Dzakiyah Dzakiyah
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引用次数: 0

摘要

研究了南苏门答腊岛拉哈县登波帕加尔阿拉姆茶园茶园废弃物中寄生茶角蛾(Tea Squrula oortina)的形式。茎和叶样品然后干燥和粉末,然后用70%的水乙醇分离。然后将提取液在70℃下真空蒸馏干燥,直到得到不含乙醇的浓缩提取液。研究结果为茶寄生提取物,并对其进行了植物化学成分(单不饱和脂肪酸和双、可可碱衍生物、槲皮素衍生物和表茶金衍生物)的检测。接下来,它将被带到Gajah Mada大学的生物科学实验室,在对照阳性阿霉素的情况下,测试其对海拉细胞的细胞毒性作用。研究结果为茶叶提取物IC50 = 2538 ug/mL,阿霉素IC50 = 0.88 ug/mL,由此得出茶寄生菌具有细胞毒性和增殖作用。
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Cytotoxicity Effects of Benalu Tea Extract (Scurrula oortina) on Hella cells
Tea garden waste has been investigated in the form of parasite tea Squrula oortina Species Family: Loranthaceae from the Dempo Pagar Alam tea plantation, Lahat Regency, South Sumatra. Stem and leaf samples are then dried and powdered, then isolated with 70% water-ethanol subfraction. The extract was then dried by vacuum distillation at 70oC until a concentrated extract without ethanol was obtained. The results of the study were tea parasite extract, and the extract was tested for phytochemicals (monounsaturated fatty acids and double, theobromine derivates, quercetin derivates and Epicatekin derivates). Next, it would be taken to the Laboratory of Biological Sciences at Gajah Mada University to be tested for cytotoxic effects on Hella cells, with control positive doxorubicin. The results of the research were tea leaves extract IC50 = 2538 ug/mL and the standard of doxorubicin IC50 = 0.88 ug/mL so that it could be concluded that the parasite of the tea parasite had cytotoxic and proliferative effects.
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