马库尔迪市肉鸡中四环素残留量的测定

J. O. Bosha, C. Akwuobu, P. Onyeyili
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摘要

采用微生物法测定肉鸡组织中四环素的残留量。从马库尔迪大都会的五个不同农场共收集了400个样本,分别代表100个肝脏、肾脏、肺和乳房肌肉样本。分析显示,总残留率为70%,其中肝脏为60%,肾脏为31%,肺部为14%,肌肉为5%。1号养殖场肝脏中浓度最高,为6 μg/kg,发生率为50%,其中肝脏占90%。农场2的发病率为75%,其中肝脏占50%。农场3的发病率为100%,其中肝脏占75%。农场4的发病率为75%,所有病例均累及肝脏。5号农场有50%的发病率,全部来自肝脏和肾脏。尽管发病率高于70%,但均显著(p < 0.01)低于肝脏、肾脏和肌肉的推荐最大残留限量(MRL) 600、300和200 μg/kg。这些数值也符合食品法典推荐的每日可接受摄入量(ADI) 0-30 μg/kg。这可能表明马库尔迪大都会选定的农场在良好的农业管理中合理使用了抗生素。值得注意的是,这表明该方法可以用数学方法量化低收入地区的药物残留。
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Determination of Tetracycline Residues in Broilers Reared in Makurdi Metropolis
Tetracycline residues in broiler tissues were assayed using a microbiological assay method. A total of four hundred samples representing one hundred samples of liver, kidney, lungs and breast muscles each, were collected from five different farms within Makurdi Metropolis. The analysis showed a total of 70 % residue incidence with the liver having 60 %, Kidney 31 %, lungs 14 % and muscles 5 % of the total. The highest concentration, of 6 μg/kg, was seen in the liver from farm 1, which had an incidence of 50 %, with the liver contributing 90 % of the incidence. Farm 2 had an incidence of 75 %, in which the liver contributed with 50 % of the cases. Farm 3 had a 100 % incidence, with the liver contributing with 75 % of the cases. Farm 4 had 75 % incidence, with the liver involved in all cases. Farm 5 had a 50 % incidence, all from the liver and kidney. Despite the higher incidence of 70 %, all the values were significantly (p < 0.01), lower than the recommended Maximum Residue Limits (MRL) or Tolerance of 600, 300 and 200 μg/kg for the liver, kidney and muscle respectively. The values were also in accordance within the Acceptable Daily Intake (ADI) of 0-30 μg/kg, recommended by the Codex Alimentarius. This may be an indication of the rational use of antibiotics in good agricultural management in the selected farms in Makurdi Metropolis. Significantly, it shows that this method can be used mathematically to quantify drug residues in lower income areas.
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