三光子荧光显微镜中的图像形成

C. Sheppard
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引用次数: 16

摘要

对三光子荧光显微成像进行了分析。考虑了点扩散函数、三维光学传递函数和轴向分辨率。结果推广到多光子荧光。三光子荧光特别适用于通过使用瞳孔滤光片来提高分辨率。
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Image formation in three‐photon fluorescence microscopy
Image formation in three-photon fluorescence microscopy is analysed. The point spread function, three-dimensional optical transfer function and axial resolution are considered. The results are generalized to multiphoton fluorescence. Three-photon fluorescence is particularly amenable to resolution improvement by use of pupil filters.
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