不同禽源肠炎沙门氏菌系统发育树及基因序列比较

H. Labib
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摘要

沙门氏菌是一种在禽类中引起高发病率和死亡率的细菌。本研究对来自各省(吉萨、开罗、达米埃塔和卡夫勒谢克)的患病和显然健康的鹌鹑的总共100个样本进行了研究。收集的样本包括不同的器官(肝、心、肺和骨髓)。并比较了从鸡鸭和兔身上分离的沙门氏菌的基因树和基因序列。细菌学检查结果显示,在采集的100份样品中,沙门氏菌分离阳性22份(22%)。沙门氏菌的血清分型主要有肠炎沙门氏菌(27.3%);Senftenberg鼠伤寒沙门氏菌(各22.8%);Agona沙门氏菌(18.1%)和Magherafelt沙门氏菌(9%)。鹌鹑沙门氏菌的药敏试验结果。结果显示,对萘啶酸和链霉素耐药的菌株(72.8%,68.2%),对庆大霉素、甲氧苄啶-磺胺甲恶唑敏感的菌株(68.2%)。此外,所有沙门氏菌分离株均表现出多药耐药。我们选择了3株菌株进行测序,测序号为MT267777 ~ MT267779。来自中国、韩国和英国的肠炎血清型菌株,鉴定率为100%。
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COMPARISON OF PHYLOGENIC TREE AND GENE SEQUENCE OF SALMONELLA ENTERITIDIS ISOLATED FROM DIFFERENT BIRDS
: Salmonella is bacterium causing high morbidity and mortality rates in the birds’. The present study was performed on a total of 100 samples from diseased and apparently healthy quail came from governorates (Giza, Cairo, Damietta and kafrelsheik). Collected samples included different organs (liver, heart, lung, and bone marrow). And a comparison between the genetic tree and gene sequences of Salmonella isolated from {chicken-ducks and rabbit}. The bacteriological examination revealed that out of 100 collected samples, 22 samples (22 %) were positive for Salmonella isolation. the serotyping of Salmonella species isolates showed a major variety of serotypes which included Salmonella Enteritidis (27.3%); Salmonella Typhimurium Senftenberg (22.8% for- each ); Salmonella Agona, (18.1%) and Salmonella Magherafelt (9%) . Results of the sensitivity testing of salmonella isolated from quail. Showed that (72.8%, 68.2%) of strains were resistant to Nalidixic acid and Streptomycin, but sensitive to Gentamicin, Trimethoprim-sulfamethoxazole, (68.2%) of strains. Also, all Salmonella isolates showed multidrug resistance. We selected three strains to be sequenced with accession number MT267777 to MT267779 and it resembled Salmonella enterica subsp. enterica serovar Enteritidis strain from China, Korea, and UK with 100% identitiy percent.
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