HPTLC AND GC-MS Analysis of Methanolic Extracts of Samanea Saman (Albizia Saman) (Jacquin) F. Mueller

Samanea saman (Albizia saman) is a wide-canopied tree with a large symmetrical crown. It usually reaches a height of 25 m (82 ft) and a diameter of 40 m. The leaves fold in rainy weather and in the evening, hence the name "rain tree" and "five o'clock tree" (Pukul Lima) in Malay. In the present investigation, the bioactive methanolic fraction was subjected to HPTLC and GC-MS analysis. GCMS analysis revealed the presence of six compounds from the methanolic fraction of the Samanea saman (1) 9,Octadecenoic acid (Z) methyl ester, (2) Dodecanoic acid, 10-methyl, methyl ester, (3) 13Hexyloxyacylotridec-10-en-2-one, (4) 15Octadecenoic acid, methyl ester (5) Diethyl -1(8 –amino -1naphthyl) -1,2,3 triazole -4,5 –dicarboxylate were identified from the methanolic extract from the leaves of Samanea saman. The presence of these compounds was confirmed by NIST library values. HPTLC analysis concludes the presence of four major phytoconstituents which was eluted at Rf 0.67; 0.79; 0.85 & 0.90 respectively.