喀麦隆西北地区非正规种子系统生产的马铃薯种块茎中六种病毒的流行情况

P A. Njukeng, G M. Chewachong, P. Sakwe, G. Chofong, L W Nkeabeng, P. Demo, K. D. Njualem
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引用次数: 6

摘要

马铃薯(Solanum tuberosum L.)是一种重要的经济粮食作物,在喀麦隆五个农业生态区中的三个广泛种植。对从喀麦隆西北地区4个种子库收集的1175个不同直径的马铃薯发芽种块茎进行了PVA、PLRV、PVM、PVS、PVX和PVY的流行病学研究。该研究还在种植前对小、中、大块茎的病毒发病率进行了分类确定。采用DAS-ELISA法检测这些病毒的存在。结果显示,6种病毒的流行率差异显著,其中PVM最流行(92%),PLRV最不流行(35%)。Upper-Farm商店的相对患病率最高(82%),所有块茎中均检测到PVM,而Rock-Farm商店的样品相对发病率最低(55%),未检测到PLRV。病毒在小块茎中最普遍(71%),在大块茎中最少(63%)。我们的研究结果表明,块茎大小可以作为鉴定健康(无病毒)块茎的指南。因此,农民可以使用大个头的马铃薯块茎进行种植。
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Prevalence of six viruses in potato seed tubers produced in informal seed system in the north west region of Cameroon
Potato ( Solanum tuberosum L.) is an important cash-food crop, which is widely grown in three of the five agro-ecological zones of Cameroon. A study was carried out to determine the prevalence of PVA, PLRV, PVM, PVS, PVX and PVY in 1175 sprouted potato seed tubers of different diameters collected from four seed stores in the North West Region of Cameroon. The study also sorts to ascertain variations in virus incidence in small, medium and large size sprouted tubers prior to planting. The DAS-ELISA method was used to test for the presence of these viruses. Results showed significant differences in the prevalence of the six viruses with PVM being the most prevalent (92%), while PLRV was least prevalent (35%) in all the four seed stores sampled.  Upper-Farm store registered the highest relative prevalence (82%), with PVM detected in all the tubers while samples from Rock-Farm store showed the least relative incidence (55%) with no PLRV detected. Viruses were significantly most prevalent in small size tubers (71%) and least in large size potato tubers (63%). Our results suggest that tuber size can serve as a guide to identify healthy (virus-free) tubers. Farmers could therefore use large size potato tubers for planting.
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