巴士拉省牛角膜结膜炎感染相关细菌的遗传检测

Nawres N. Jaber, M. H. Sayhood, N. Hadi, Basil A. Abbas, Noor Amjad Kazem
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摘要

兽医用化学品是用于治疗或预防疾病、伤害和害虫的药物或治疗方法。如果没有兽药,牲畜的生存和生产力将严重降低,特别是像猪和家禽这样集约化饲养的动物。传染性牛角膜结膜炎是牛的一种细菌性眼病,对巴士拉省受细菌病原体污染的动物眼睛的研究了解有限。因此,本研究的目的是分离和分类一些与牛角膜结膜炎感染相关的细菌种类。这项研究包括对来自巴士拉省不同地区的120头奶牛的眼拭子进行检查。目前的研究是在2018年10月至2019年2月期间进行的。在本研究涉及的120例病例中,有30例被确定为角膜结膜炎。研究结果表明,奶牛的眼部感染多为单侧感染(69%),双侧感染较少(30.9%)。经16S rDNA和测序鉴定,分离出的细菌分别为大肠杆菌40%、枯草芽孢杆菌10%、癌症肠杆菌属10%、霍氏肠杆菌10%、阴沟肠杆菌15%、肺炎克雷伯菌15%。
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Genetic Detection of Some Bacterial Species Associated with Bovine Keratoconjunctivitis Infections in Basra Governorate
Veterinary chemicals are pharmaceuticals or treatments that are used in cattle to treat or prevent disease, injury, and pests. Livestock survival and productivity would be severely reduced without veterinary drugs, especially for intensively maintained animals like pigs and poultry. Infectious bovine keratoconjunctivitis is a bacterial eye illness of cattle, and there is limited knowledge on the study of bacterium pathogen-contaminated eyes of animals in Basra province. As a result, the purpose of this research was to isolate and classify some bacterial species associated with infection in cattle with keratoconjunctivitis. This study included examination of 120 eye swabs from cows from different regions in the Basra governorate. The current study was carried out from October 2018 to February 2019. Out of 120 cases involved in this study, 30 cases were identified to have keratoconjunctivitis. The results of the study showed that eye infections in cows were mostly unilateral, in one eye (69%), and less than bilateral infections in both eyes (30.9%). Twenty different bacterial isolates were obtained, and the isolated bacteria were identified genetically by 16S rDNA and sequencing as Escherichia coli 40%, Bacillus subtilis 10%, Enterobacter cancer genus 10%, Enterobacter hormaechei 10%, Enterobacter cloacae 15%, and Klebsiella pneumonia 15%.
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