凤凰素-20对斑马鱼胰岛素样生长因子系统mrna的肝脏和肌肉特异性影响

IF 1.6 4区 医学 Q4 CELL BIOLOGY Growth Hormone & Igf Research Pub Date : 2022-04-01 DOI:10.1016/j.ghir.2022.101456
Jithine Jayakumar Rajeswari , Emilio J. Vélez , Suraj Unniappan
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引用次数: 1

摘要

目的研究凤凰素-20 (phoenixin -20, Pnx-20)是一种具有内分泌样作用的生物活性肽。最近的研究表明,Pnx-20促进生长激素/胰岛素样生长因子(Gh/Igf)轴,这是哺乳动物和鱼类生长的重要内分泌调节因子。在本研究中,我们确定了Pnx-20是否影响斑马鱼肝脏和肌肉中Igf家族的不同成员、其结合蛋白和受体(Igf系统)。结果Pnx-20在腹腔注射后1和6 h下调雄性和雌性斑马鱼肝脏中igfs、igf受体(igfrs)和igf结合蛋白(igfbp) 5mrna的表达。有趣的是,这种效应发生在雌性斑马鱼相对较早的时间点,这表明Pnx-20的作用存在性别特异性差异。此外,Pnx-20体外培养6或24小时后,斑马鱼肝细胞(ZFL)中除igf2a外,所有igfs、igfrs和igfbp5 mrna的表达均下调。此外,sirna介导的Pnx-20的下调上调了ZFL细胞中分析的所有igf系统mrna。总之,这些结果(体内和体外)揭示了内源性和外源性Pnx-20对斑马鱼肝脏igf系统的普遍抑制作用。相比之下,在注射Pnx-20的鱼的肌肉中发现了igf系统mrna的性别特异性上调。未来的研究应该探索在本研究中观察到的性别和时间差异。总之,本研究表明Pnx-20在雄性和雌性斑马鱼中都是肝脏(抑制因子)和肌肉(刺激因子)Igf信号的组织特异性调节因子。
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Liver and muscle-specific effects of phoenixin-20 on the insulin-like growth factor system mRNAs in zebrafish

Objective

Phoenixin-20 (Pnx-20) is a bioactive peptide with endocrine-like actions in vertebrates. Recent studies suggest Pnx-20 promotes growth hormone/insulin-like growth factors (Gh/Igf) axis, an important endocrine regulator of growth in mammals and fish.

Design

In this research, we determined whether Pnx-20 affects the different members of the Igf family, its binding proteins and receptors (Igf-system) in zebrafish liver and muscle.

Results

In vivo administration of Pnx-20 downregulated igfs, igf receptors (igfrs) and igf binding protein (igfbp) 5 mRNA expression in the liver of male and female zebrafish at both 1 and 6 h post-intraperitoneal (IP) injection. Interestingly, this effect occurred at a relatively earlier timepoint in female zebrafish suggesting sex-specific differences in Pnx-20 action. Besides, either 6 or 24 h in vitro incubations with Pnx-20 downregulated the expression of all igfs, igfrs and igfbp5 mRNAs (except igf2a) analyzed in a zebrafish liver cell (ZFL) line. Moreover, siRNA-mediated knockdown of Pnx-20 upregulated all Igf-system mRNAs analyzed in ZFL cells. Together, these results (both in vivo and in vitro) revealed a general suppressive action for both endogenous and exogenous Pnx-20 on the hepatic Igf-system of zebrafish. In contrast, a general sex-specific upregulation of the Igf-system mRNAs analyzed was found in the muscle of Pnx-20 injected fish. Future research should explore the sex- and time-differences observed in the present study.

Conclusions

Collectively, this research shows that Pnx-20 is a tissue-specific regulator of the liver (suppressor) and muscle (stimulant) Igf signaling in both male and female zebrafish.

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来源期刊
Growth Hormone & Igf Research
Growth Hormone & Igf Research 医学-内分泌学与代谢
CiteScore
3.30
自引率
0.00%
发文量
38
审稿时长
57 days
期刊介绍: Growth Hormone & IGF Research is a forum for research on the regulation of growth and metabolism in humans, animals, tissues and cells. It publishes articles on all aspects of growth-promoting and growth-inhibiting hormones and factors, with particular emphasis on insulin-like growth factors (IGFs) and growth hormone. This reflects the increasing importance of growth hormone and IGFs in clinical medicine and in the treatment of diseases.
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