一氧化氮合酶和环氧合酶在对乙酰氨基酚肝毒性发展中的相互作用

M. Kolgazi, Ünal Uslu, M. Yüksel, Ayliz Velioğlu Öğünç, F. Ercan, I. Alican
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引用次数: 1

摘要

目的:大剂量对乙酰氨基酚(扑热息痛、n -乙酰基-对氨基酚)的误服;已知APAP可引起中毒性肝炎。本研究旨在探讨一氧化氮合酶(NOS)和环氧合酶(COX)系统在apap诱导大鼠肝毒性过程中的相互关系。材料与方法:Sprague-Dawley大鼠腹腔注射APAP (500 mg/kg)或与非选择性NOS抑制剂N ω -硝基- l -精氨酸甲酯盐酸(L-NAME)联合注射;20 mg/kg), iNOS抑制剂氨基胍(AG;8 mg/kg),非选择性COX抑制剂吲哚美辛(indomethacin;5 mg/kg),选择性COX-2抑制剂尼美舒利(NIM;10 mg/kg)和选择性COX-1抑制剂酮咯酸酯(KET;5毫克/公斤)。APAP给药24 h后,取肝脏和血液标本进行生化和形态学评价。结果:AG和KET抑制了APAP毒性引起的肝脏丙二醛水平升高。除NIM外,APAP组肝脏谷胱甘肽水平均未降低。除INDO和NIM外,APAP组受刺激的肝髓过氧化物酶活性均减弱。APAP过量引起的肝脏化学发光、核因子- κ B表达和血清丙氨酸转移酶水平升高均受到抑制。结论:NOS和COX通路在APAP过量肝毒性发生过程中相互作用。
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Interaction between nitric oxide synthase and cyclooxygenase in the development of acetaminophen-induced hepatotoxicity -
Objective: Ingestion of high doses of acetaminophen (paracetamol, N-acetyl- p -aminophenol; APAP) is known to cause toxic hepatitis. This study aimed to examine the interrelationship between nitric oxide synthase (NOS) and cyclooxygenase (COX) systems in the course of APAP-induced hepatotoxicity in the rat. Materials and Methods: Sprague-Dawley rats were injected intra-peritoneally with APAP (500 mg/kg) alone or along with the non-selective NOS inhibitor N ω -Nitro-L-arginine methyl ester hydrochloride (L-NAME; 20 mg/kg), iNOS inhibitor aminoguanidine (AG; 8 mg/kg), non-selective COX inhibitor indomethacin (INDO; 5 mg/kg), selective COX-2 inhibitor nimesulide (NIM; 10 mg/kg) and selective COX-1 inhibitor ketorolac (KET; 5 mg/kg). 24 h after APAP administration, the liver and blood samples were collected for biochemical and morphological evaluations. Results: AG and KET prevented the increase in liver malondialdehyde levels due to APAP toxicity. Decreased liver glutathione in APAP group was prevented by all treatments except NIM. Stimulated liver myeloperoxidase activity in APAP group was attenuated by all treatments except INDO and NIM. Elevation of liver chemiluminescence, nuclear factor- κ B expression and serum alanine transferase level due to APAP overdose were also suppressed by all treatments. Conclusions: NOS and COX pathways interact in the development of hepatotoxicity due to APAP overdose.
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