某些本土植物提取物对茶叶红蜘蛛螨、咖啡少爪螨、叶螨的生物功效研究

B. Deka, A. Babu, M. Sarmah
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引用次数: 9

摘要

在室内对照条件下,采用叶盘法测定了五种不同当地植物水蓼、牡荆花、野生向日葵、寻常蒿和金盏花叶提取物对茶红蜘蛛螨、咖啡少爪螨和叶螨的杀卵和杀螨活性。将上述植物的叶片遮阳干燥,在电动搅拌器中研磨,用己烷和甲醇两种不同的溶剂提取,用二甲基亚砜(DMSO)稀释,得到1%、0.75%、0.50%和0.25%的不同浓度;一种重要的极性溶剂),并用于不同的生物测定。在喷洒后评估了提取物对成虫和虫卵死亡率的影响,并将结果与未处理的对照进行了比较。与未经处理的对照相比,所有被试植物对红蜘蛛螨的功效都有不同程度的变化。在5种被试植物中,黄荆和黄竹甲醇提取物的杀卵作用最大(分别为66.71%和57.45%),在1%浓度下成虫死亡率超过90%。黄荆和黄荆的己烷和甲醇提取物在24 h后的作用。
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Bio-efficacy of Certain Indigenous Plant Extracts against Red Spider Mite, Oligonychus coffeae , Nietner (Tetranychidae: Acarina) Infesting Tea
Leaf extracts of five different locally available plants such as Polygonum hydropiper  L. , Vitex negundo L , Tithonia diversifolia (Hemsl.) (wild sun flower), Artemisia vulgaris L and Clerodendrum infortunatum L were evaluated for their ovicidal and acaricidal activity against the tea red spider mite, Oligonychus coffeae , Nietner (Tetranychidae: Acarina) in the laboratory using the leaf disc method under controlled conditions. Leaves collected from the above mentioned plants were dried under shade, milled in an electric blender and extracted using two different solvents, hexane and methanol and prepared different concentrations viz. 1%, 0.75%, 0.50% and 0.25% by diluting in DMSO (dimethyl sulfoxide; an important polar solvent) and were used for different bioassays. The effects of the extracts on the mortality of adults as well as in the eggs were assessed after spraying and the results were compared with an untreated control. All tested plants showed varying levels of efficacy on red spider mite compared when with the untreated control. Among five plant species tested, methanol extract of Vitex negundo and Clerodendrum infortunatum showed maximum ovicidal action (66.71% and 57.45% respectively) and exhibited more than 90% adult mortality @ 1% conc. of both the hexane and methanol extract of Vitex negundo and Clerodendrum infortunatum after 24 h.
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