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Biodegradation of Camellia sinensis (L.) O. Kuntze Wood by Hypoxylon sp. Isolates from Diverse Tea Growing Counties of Kenya 茶树的生物降解研究肯尼亚不同产茶县的低氧梭菌(Hypoxylon sp.)分离株
Pub Date : 2020-09-30 DOI: 10.20425/IJTS1511
J. Langat, R. Ramkat, R. Mose
Many fungi play a vital role in the decomposition of wood in nature and nutrient cycling, yet some afflict serious damage to cultivated tree crops and forestry. Tea (Camellia sinensis (L) O. Kuntze) is affected by Hypoxylon wood rot (HWR) disease caused by the Hypoxylon sp. Pers ex Fr. In the recent years; there have been reports on increase of the disease in Kenya. This study aimed at determining disease incidence and biodegradation by 59 Hypoxylon isolates from diverse tea growing counties of Kenya on wood, from resistant (TRFK 6/25) and susceptible (TRFK 6/129) tea cultivars. The disease incidence varied (p less than or eqaul to 0.05) in different counties (6.7 to 77.5%) and was more prevalent in tea cultivars (77.5%) than seedling type of teas (15.7%). Bio-degradation of wood was performed in Falcon tubes for 40 days. The 59 Hypoxylon isolates significantly (p less than or eqaul to 0.05) bio-degraded tea wood. The weight loss ranged from 4.84 to 16.44% in the susceptible and 4.61 to 12.64% in the resistant cultivars. The results indicate the potential use of biodegradation to evaluate resistance of tea cultivars to damage by Hypoxylon sp. This study concludes that biodegradation is potentially usable technique to screen tea cultivars for resistance to HWR.
许多真菌在自然界木材的分解和养分循环中起着至关重要的作用,但有些真菌对栽培的树木作物和林业造成严重损害。近年来,茶树(Camellia sinensis (L) O. Kuntze)遭受了由矮杉木(Hypoxylon sp. Pers ex Fr.)引起的木腐病(HWR)。有报道称肯尼亚的疫情有所增加。本研究旨在测定来自肯尼亚不同产茶县的59株耐药(TRFK 6/25)和易感(TRFK 6/129)茶叶品种的低氧ylon在木材上的发病率和生物降解情况。各县发病率差异显著(p < 0.05)(6.7 ~ 77.5%),以茶树品种发病率(77.5%)高于茶树苗型发病率(15.7%)。木材的生物降解在Falcon试管中进行了40天。59株Hypoxylon对茶木有显著降解(p≤0.05)。易感品种的失重幅度为4.84 ~ 16.44%,抗性品种的失重幅度为4.61 ~ 12.64%。研究结果表明,生物降解技术可用于茶叶品种对褐枯病菌的抗性评价,是筛选茶叶品种对褐枯病菌抗性的有效技术。
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引用次数: 0
Cloning and Expression Analysis of Chloroplast-targeted Ferredoxin-NADP+ Oxidoreductase Gene of Tea Plant (Camellia sinensis) cv.Huangjinya 茶树叶绿体靶向铁氧化还原蛋白nadp +氧化还原酶基因的克隆与表达分析Huangjinya
Pub Date : 2020-01-01 DOI: 10.5376/jtsr.2020.10.0001
Xiuxiu Zhao, Yangen Fan, Yueyue Tian, Hanyue Wang, Lixia Zhang, Min Li
The chloroplast-targeted ferredoxin-NADP+ oxidoreductase (LFNR) is a hydrophilic flavin protein located at the end of the photosynthesis electron delivery chain in higher plants. It’s a hub for linking photosynthesis electronic transportation with chloroplast redox metabolism. In order to explore the relationship between LFNR gene and yellowing of ‘Huangjinya’controlled by light, we took separately one bud two leaves of ‘Huangjinya’ ‘Shuchazao’(CK) as the material to clone CsLFNR gene and then we obtained the sequence with a similarity of 100%, named CsLFNR1.1 (GenBank accession no. MT311318). Throw bioinformatics analysis, we know its cDNA gene length is 1 095 bp, coding 364 amino acids, The protein molecular weight is 40.623KDa, the theoretical isoelectric point is 8.86.CsLFNR1.1 is a alkaline protein without transmembrane structure and signal peptide, it has a chloroplast transport peptide (cTP), the secondary structure contains 23.35% alpha helix, 5.49% beta-corner, 28.85% extension chain and 42.31% irregular curling, It locates in the chloroplast. Through the Nucleotide BLAST, Protein BLAST and DNAMAN software, we found that CsLFNR1.1 and its translated amino acid sequences had 4 bases and 3 amino acid differences with NCBI ‘Shuchazao’ Gene CsLFNR1 (XM_028233617.1) and Protein (XP_028089418.1), the similarity reached 99.63% and 99.18%. CsLFNR1.1 and CsLFNR1 have small differences in the physical and chemical properties and secondary structure, the tertiary structure prediction template of them is consistent. In addition, they have the same conserved domain and active site. We took different shade ‘Huangjinya’ blades as the material for qRT-PCR, the result showed that the expression of CsLFNR1.1 gene response to the light intensity, its expression increased with the increase of light intensity. This study provides a theoretical basis and scientific basis for further exploring the role of CsLFNR1.1 gene in the light regulation process of the new shoots and leaves of ‘Huangjinya’.
叶绿体靶铁氧化还原酶是一种位于高等植物光合作用电子传递链末端的亲水黄素蛋白。它是连接光合作用电子传输和叶绿体氧化还原代谢的枢纽。为了探究LFNR基因与‘黄金牙’‘Shuchazao’(CK)光控黄变的关系,我们分别以‘黄金牙’‘Shuchazao’(CK)的一芽两叶为材料克隆了CsLFNR基因,得到了相似度为100%的序列,命名为CsLFNR1.1 (GenBank登录号:MT311318)。通过生物信息学分析,我们知道其cDNA基因长度为1 095 bp,编码364个氨基酸,蛋白质分子量为40.623KDa,理论等电点为8.86。cslfnr1.1是一种碱性蛋白,无跨膜结构和信号肽,具有一个叶绿体转运肽(cTP),二级结构包含23.35%的α螺旋、5.49%的β角、28.85%的延伸链和42.31%的不规则卷曲,位于叶绿体中。通过Nucleotide BLAST、Protein BLAST和DNAMAN软件,我们发现CsLFNR1.1及其翻译的氨基酸序列与NCBI‘Shuchazao’基因CsLFNR1 (XM_028233617.1)和Protein (XP_028089418.1)有4个碱基和3个氨基酸的差异,相似度分别达到99.63%和99.18%。CsLFNR1.1和CsLFNR1在理化性质和二级结构上差异不大,三级结构预测模板一致。此外,它们具有相同的保守结构域和活性位点。我们以不同遮荫的‘黄金崖’叶片为材料进行qRT-PCR,结果表明CsLFNR1.1基因的表达对光强有响应,其表达量随着光强的增加而增加。本研究为进一步探索CsLFNR1.1基因在‘黄金牙’新芽新叶光调控过程中的作用提供了理论基础和科学依据。
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引用次数: 0
Analysis of DNA Barcoding Suitable for Tea Tree Field Genebank 适于茶树大田基因库的DNA条形码分析
Pub Date : 2020-01-01 DOI: 10.5376/JTSR.2020.10.0002
Yanyan Li, Wei Huang, Jia-Sian Lin, Chuanpeng Nie
With the development of research for DNA barcoding, its application has attracted more and more attention. In this study, 100 tea tree samples were selected as subjects, the partial sequences of chloroplast matK and rbcL genes were used to investigate the molecular barcodes suitable for tea tree The results showed that the rbcL sequences of 100 tea samples were identical, the matK sequences were different, the genetic distance ranged from 0.000 to 0.032, the sequences could be divided into 14 haplotypes, Hd and Pi were 0.604 and 0.23×10-2, at the same time, the construction analysis of the sequence is carried out. The results showed that the matK sequence could be used in the development and utilization of DNA barcoding of tea tree field genebank.
随着DNA条形码研究的深入,其应用越来越受到人们的重视。本研究以100棵茶树样品为研究对象,利用叶绿体matK和rbcL基因的部分序列对适合茶树的分子条形码进行了研究。结果表明,100棵茶树样品的rbcL序列相同,而matK序列不同,遗传距离在0.000 ~ 0.032之间,序列可划分为14个单倍型,Hd和Pi分别为0.604和0.23×10-2。对序列进行了结构分析。结果表明,该matK序列可用于茶树大田基因库DNA条形码的开发和利用。
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引用次数: 0
Genetic Diversity Analysis of Youxi Bitter Tea Resources Based on ISSR Molecular Markers 基于ISSR分子标记的幽溪苦茶资源遗传多样性分析
Pub Date : 2020-01-01 DOI: 10.5376/jtsr.2020.10.0003
Shasha Wei, J. Peng, Zhidan Chen, Weijiang Sun, Lin Lin, Renquan Wu
In this study, we analyzed the genetic diversity by using ISSR markers in four populations with 37 DNA samples of Youxi Kucha. In total, 66 alleles were amplified using 8 ISSR primers. A total of 66 bands were detected, of which 63 bands were polymorphic with a polymorphic proportion of 95.24 %. At the population level, the average PPL of the four populations of Youxi Kucha is 76.19 %, and the average values of Nei's gene diversity index (H) and Shannon diversity index (I) are 0.204 5 and 0.367 7 respectively. At the species levelm the H and I are 0.305 1 and 0.465 0 respectively respectively. Youxi Kucha maintained a relatively high genetic variability at the population level and species level bothly. The genetic differentiation within the population was significantly higher than that between populations,but the genetic differentiation between the populations has also reached higher level (Φst = 0.17> 0.15), the differentiation is extremely significant (P <0.01). A mantel test indicated there was no significant relationship between genetic distance and geographic distance among the populations studied. Correlation analysis shows that the diversity index has little relationship with altitude. This study carried out the molecular identification of the germplasm resources of Youxi bitter tea accurately, which can provide a theoretical basis for the protection of Youxi bitter tea and the breeding of improved tea varieties.
本研究利用ISSR标记分析了油溪苦茶4个居群的遗传多样性。共扩增到66个等位基因,共使用8条ISSR引物。共检测到66条条带,其中多态性条带63条,多态性比例为95.24%。在种群水平上,油溪库茶4个种群的平均PPL为76.19%,Nei’s基因多样性指数(H)和Shannon多样性指数(I)的平均值分别为0.204 5和0.367 7。在种水平上,H和I分别为0.305 1和0.465 0。油溪库茶在种群水平和种水平上均保持较高的遗传变异性。居群内遗传分化显著高于居群间,居群间遗传分化也达到了较高水平(Φst = 0.17> 0.15),分化极显著(P <0.01)。种群间遗传距离与地理距离的关系不显著。相关分析表明,多样性指数与海拔高度关系不大。本研究对幽溪苦茶种质资源进行了准确的分子鉴定,可为幽溪苦茶的保护和茶叶良种的选育提供理论依据。
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引用次数: 0
Cartosat-1 Image Segmentation Technique for Shade Tree Crown Density in Tea Gardens of East India in Relation to Terrain Geometry 基于地形几何关系的东印度茶园遮荫树冠密度制图技术
Pub Date : 2018-06-14 DOI: 10.5376/jtsr.2018.08.0001
D. Dutta, Libeesh Lukose, A. Bajpai, Uttam Kumar Bhunia, R. Singh, Sourav Samanta
One of the factors determining tea quality is shadow casting by the shade trees. Besides regulating incoming solar radiation shade trees also helps maintaining the moisture in soil and nutrient recycling. However the optimum shade density depends upon the elevation, slope and aspect. In the present study image segmentation technique was employed on Cartosat-1 data to capture the vertical crown density of the shade trees. Significant positive correlations (r 2 =0.91) were found between observed and measured vertical crown density. Based upon the crown density the tea gardens were classified. Further the relation between crown density and terrain parameters has been analysed. Significant negative correlation was observed with elevation (-0.590) and slope (-0.627) which indicates that to increase in elevation and/or percent slope the shade density decreases.
决定茶叶品质的因素之一是遮荫树的阴影。除了调节太阳辐射外,遮荫树还有助于保持土壤水分和养分循环。然而,最佳遮荫密度取决于海拔、坡度和坡向。本研究采用图像分割技术,对Cartosat-1数据进行垂直树冠密度的提取。垂直树冠密度与观测值呈显著正相关(r 2 =0.91)。根据树冠密度对茶园进行分类。进一步分析了树冠密度与地形参数的关系。与海拔高度(-0.590)和坡度(-0.627)呈显著负相关,表明随着海拔高度和坡度百分比的增加,遮荫密度降低。
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引用次数: 3
Heterosis for Catechins and Caffeine in Kenyan Tea (Camellia sinensis) (L.) O. Kuntze). 肯尼亚茶(Camellia sinensis)儿茶素和咖啡因的杂种优势o . Kuntze)。
Pub Date : 2018-03-01 DOI: 10.20425/IJTS.V13I01-02.11397
Nelson M. Lubang’a, S. Kamunya, O. Kiplagat, J. Wanyoko, R. Chalo
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引用次数: 0
Organic Tea: Global Market and Forecast Sales 有机茶:全球市场和预测销售
Pub Date : 2017-12-29 DOI: 10.5376/jtsr.2017.07.0011
N. Hajra
The increasing demand from the consumers of Germany, United Kingdom, Japan and USA for organic teas, few conventional tea gardens of India, Sri Lanka and China have converted to organic processes during 1980s and subsequently it has been spread to different tea producing countries. Still the organic tea sector is a very small part of the tea industry, accounting of less than 1.3% by volume but the number of organic tea producers and the volume of organic tea traded in the world market have recorded high growth over the last couple of years. The annual growth rate has since cooled to 6%. It has a niche market where the produce sells at a premium price. The global organic tea market can be segmented into North America, Europe, Asia Pacific and rest of the world. Asia-Pacific region has the major market share followed by Europe. The domestic market for organic tea does not yet exist in India and Sri Lanka. But there is strong domestic market for organic green tea in China. China is exporting organic tea to Japan, the Europe and the United States. India leads the world in organic black tea output. Based on the higher demand for healthy beverages, the import and export of organic tea in the developed countries is found to rise at a steady growth rate. Around 75-80% of organic teas were exported to the United States and Germany followed by France, Denmark, the United Kingdom, Japan and Holland. In the US, organic tea is the fastest growing section of the tea industry and North America was at the peak of a growth burst in 2015, reinforcing its importance as the largest global market in terms of region, with Western Europe and Asia Pacific lagged slightly behind. Different global market research firm have predicted that the organic tea will continue to dominate and expected to increase by a CAGR of 5-13% next five years in U.S. In the present paper, the development of organic tea, global market and trade are discussed.
随着德国、英国、日本和美国消费者对有机茶的需求不断增加,印度、斯里兰卡和中国的一些传统茶园在20世纪80年代改用有机工艺,随后推广到不同的茶叶生产国。有机茶行业仍然是茶产业的一小部分,占不到1.3%的产量,但有机茶生产商的数量和世界市场上有机茶的交易量在过去几年中实现了高速增长。此后,年增长率降至6%。它有一个利基市场,产品以高价出售。全球有机茶市场可以分为北美、欧洲、亚太和世界其他地区。亚太地区占有主要市场份额,其次是欧洲。印度和斯里兰卡还没有有机茶的国内市场。但中国有机绿茶的国内市场非常强劲。中国正在向日本、欧洲和美国出口有机茶。印度有机红茶产量居世界首位。基于对健康饮料的更高需求,发现发达国家有机茶的进出口以稳定的速度增长。大约75-80%的有机茶出口到美国和德国,其次是法国、丹麦、英国、日本和荷兰。在美国,有机茶是茶叶产业中增长最快的部分,北美在2015年达到了增长的顶峰,巩固了其作为全球最大地区市场的重要性,西欧和亚太地区略落后。不同的全球市场研究公司预测,有机茶将继续占据主导地位,预计未来五年有机茶将以5-13%的复合年增长率增长。本文讨论了有机茶的发展,全球市场和贸易。
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引用次数: 5
Types of Indian Tea, Production and Marketing of Traditional and Handmade Teas of Assam, India 印度茶的种类,阿萨姆邦传统和手工茶的生产和销售
Pub Date : 2017-12-22 DOI: 10.5376/JTSR.2017.07.0010
P. Baruah
Teas are classified in various ways depending manufacturing process, convention, technique and market available form. An exhaustive classification based on market available forms classifies Indian teas into eleven broad categories. Assam is a place of origin of tea, wild tea plants were discovered in Assam in 1823 and tea plantations started since 1837. Some of the indigenous tribal people of Assam had the habit of drinking tea prepared in their traditional form and the Singphos call such tea as phalap. Entrepreneurs of small tea growers who are a major force to reckon with in Assam tea at present are producing organic (called natural) handmade and traditional forms of teas which are gaining popularity world over. Organic certification, technical guidance in production and marketing are some of the problems faced by the producers of the handmade and traditional teas who are almost entirely small tea growers. Such teas are of special character with high quality. Demand for these teas is growing both internally and in global market and has huge market potential. Entrepreneurial handmade and traditional tea producers are putting their efforts to popularize and market such forms of tea in India and globally overcoming the challenges.
茶叶根据制造工艺、惯例、技术和市场可获得的形式有不同的分类。根据市场上可获得的形式,将印度茶分为11大类。阿萨姆邦是茶叶的原产地,1823年在阿萨姆邦发现了野生茶树,1837年开始种植茶园。阿萨姆邦的一些土著部落居民有喝传统形式的茶的习惯,Singphos称这种茶为phalap。目前,小茶农的企业家是阿萨姆邦茶叶的主要力量,他们生产有机(称为天然)手工和传统形式的茶,这些茶在世界各地越来越受欢迎。有机认证,生产和销售的技术指导是手工茶和传统茶的生产者面临的一些问题,他们几乎都是小茶农。这种茶具有独特的品质和高品质。国内和全球市场对这些茶的需求都在增长,市场潜力巨大。具有创业精神的手工茶和传统茶生产商正在努力在印度和全球推广和销售这种形式的茶,以克服挑战。
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引用次数: 3
Management of Human Resources during Pre and Post-Globalized Regime: An Empirical Study in Tea Industry of North Bengal 全球化前与全球化后的人力资源管理:北孟加拉茶产业的实证研究
Pub Date : 2017-11-14 DOI: 10.5376/JTSR.2017.07.0009
D. Biswas, N. C. Roy
Presently, Indian tea plantation industry has been confronted stiff competition due to reduction of tariff barrier and relaxation of the quantity restriction on import because of LPG (Liberalization, Privatization and Globalization) policy promulgated in 1991. Tea estates of the North Bengal have been forced on reducing labour cost to be in stable position, not only that, due to the low profitability, most of the estates are not in a position to mitigate their responsibilities towards the workers as prescribed by the labour legislations. As a result of that the workers, who are mainly dependent on the tea plantation to survive, had to live hand to mouth. To have a two square meal in a day, they have resorted to sell their child also in different tea estates. Death of tea workers due to malnutrition in this region have become common phenomenon. All these have led to the workers to become more violent, resorts to strike to pressurize on the management, etc. have increased significantly. The present study highlights the pattern of human resource management during the pre and post globalization era in tea plantation industry in North Bengal region. Primary data has been used mainly in this study and collection of which has done with the help of stratified random sampling technique from the selected tea estates. Thereafter analysis and conclusions have made accordingly.
目前,由于1991年颁布的液化石油气(自由化、私有化和全球化)政策降低了关税壁垒,放宽了进口数量限制,印度茶业面临着激烈的竞争。北孟加拉的茶园被迫降低劳动力成本以保持稳定的地位,不仅如此,由于盈利能力低,大多数茶园无法按照劳动法的规定减轻对工人的责任。因此,主要依靠茶园生存的工人们不得不勉强糊口。为了一天吃两顿饱饭,他们不得不把孩子卖到不同的茶园。茶工因营养不良死亡在该地区已成为普遍现象。所有这些都导致工人变得更加暴力,采取罢工来向管理部门施压等方式明显增加。本研究重点研究了全球化前后孟加拉地区茶业人力资源管理模式。本研究主要使用原始资料,资料的收集采用分层随机抽样技术。随后进行了相应的分析和结论。
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引用次数: 1
Effects of Nitrogen Fertilizer Rates and Seasons on Polyphenols and Catechins of Non-aerated Green Tea Processed from Seedling Tea (Camellia sinensis) 施氮量和季节对未曝气绿茶苗茶多酚和儿茶素的影响
Pub Date : 2017-09-19 DOI: 10.20425/ijts.v13i01-02.9984
S. O. Ochanda, J. Wanyoko, D. Kamau, A. Faraj, C. Onyango
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引用次数: 1
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