GL2和TT12启动子驱动AtYUC8异位表达影响拟南芥营养生长

Chao Tan, Jia Song, Tan Zhang, Mengxiao Liang, Suxin Li, Huabin Liu, Shuzhen Men
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引用次数: 0

摘要

生长素在调节拟南芥生长发育中起着至关重要的作用。YUCCA (YUC)家族基因编码黄素单加氧酶,是生长素生物合成途径中的限速酶。先前的研究表明,YUC8过表达(YUC8 OE),以及由GL2 (GLABRA 2)和TT12 (TRANSPARENT TESTA 12)启动子驱动的YUC8异位表达(分别特异表达于表皮和种子内被)产生的种子比col大,但这些转基因品系对拟南芥营养生长的影响尚不清楚。在这里,我们发现与YUC8 OE相比,GL2pro:YUC8- gfp和TT12pro:YUC8- gfp转基因植株产生了适度的过量生长素积累表型。这两个转基因品系产生较小的莲座和叶片,较高的株高,较少的分枝和较长的叶柄。这些数据将为研究生长素合成基因异位表达与作物产量的关系提供依据。
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Ectopic Expression of AtYUC8 Driven by GL2 and TT12 Promoters Affects the Vegetative Growth of Arabidopsis
Auxin plays an essential role in regulating Arabidopsis growth and development. YUCCA (YUC) family genes encode flavin monooxygenases, which are rate-limiting enzymes in the auxin biosynthetic pathway. Previous studies showed that YUC8 overexpression (YUC8 OE), as well as ectopic expression of YUC8 driven by GL2 (GLABRA 2) and TT12 (TRANSPARENT TESTA 12) promoters, which are specifically expressed in the epidermis and inner seed integument, respectively, produced larger seeds compared to the Col. However, the impact of these transgenic lines on the vegetative growth of Arabidopsis remains unclear. Here, we show that the GL2pro:YUC8-GFP and TT12pro:YUC8-GFP transgenic plants produce a moderate excessive auxin accumulation phenotype compared to the YUC8 OE. These two transgenic lines produced smaller rosette and leaf, higher plant height, fewer branches, and longer siliques. These data will provide a basis for the study of the relationship between the ectopic expression of auxin synthesis genes and crop yield.
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