Difference in F2 Isoprotane Levels in Male Model Mice (Mus musculus L.) in Maximum Physical Exercise Without Supplementation

Vigorous physical exercise that exceeds the limit of fatigue can increase the formation of free radicals that lead to oxidative stress. One of the biomarkers used to determine the presence of oxidative stress is the level of F2isoprostane. This study aims to determine the differences in levels of F2-isoprostan in male model mice (Mus musculus L.) in maximum physical exercise without supplementation. This research is an experiment. The population in this study were 30-35gram male mice aged 8-10 weeks. Based on the provisions in the study, the sample was 30 male mice that met the criteria. Examination of samples using ELISA reader and data processed in tables and graphs. The results of statistical tests obtained p value <0.05 (p <0.01), it can be concluded that there is a difference in F2-isoprostane levels between the groups that were not given weight training and those who were given maximum weight training. Where the levels of F2-isoprostane given maximum weight, training were 2 times greater than those not given weight training.